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Electrochemical clenbuterol immunosensor based on a gold electrode modified with zinc sulfide quantum dots and polyaniline

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Microchimica Acta Aims and scope

Abstract

A nanocomposite consisting of zinc sulfide quantum dots and polyaniline (ZnSQD@PANI) was placed on a gold electrode along with antibody against clenbuterol to give an amperometric immunosensor for clenbuterol. Compared to the use of pristine PANI, the electrode modified with the ZnSQD@PANI nanocomposite adsorbs clenbuterol antibody much better and therefore exhibits higher sensitivity to clenbuterol. The biosensor, when operated at a working potential of 0.21 V (vs. Ag/AgCl), displays a detection limit as low as 5.5 pg⋅mL−1 and works over the 0.01 to 10 ng⋅mL−1 concentration range. Related species such as salbutamol and ractopamine, urine components such as urea and uric acid, and the ions Ca(II), Na(I), and K(I) do not interfere.

A nanocomposite of zinc sulfide quantum dots and polyaniline was synthesized and utilized as the electrode material for detecting clenbuterol hydrochloride (CH). Sensing includes immunoglobulin G anchoring, adsorption of clenbuterol antibody, and detection of clenbuterol.

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Acknowledgments

This work was supported by Program for the National Natural Science Foundation of China (NSFC: Account No. 51173172) and Innovative Technology Team of Henan Province (2014).

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Authors and Affiliations

  1. State Laboratory of Surface and Interface Science of Henan Province, Zhengzhou University of Light Industry, No. 166, Science Avenue, Zhengzhou, 450001, People’s Republic of China

    Zhihong Zhang, Fenghe Duan, Linghao He, Donglai Peng, Fufeng Yan & Chunxiao Jia

  2. Henan Collaborative Innovation Center of Environmental Pollution Control and Ecological Restoration, Zhengzhou University of Light Industry, No. 166, Science Avenue, Zhengzhou, 450001, People’s Republic of China

    Zhihong Zhang & Minghua Wang

  3. Henan Collaborative Innovation Center of Food Production and Safety, Zhengzhou University of Light Industry, No. 166, Science Avenue, Zhengzhou, 450001, People’s Republic of China

    Wei Zong

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  1. Zhihong Zhang
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  2. Fenghe Duan
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  3. Linghao He
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  4. Donglai Peng
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Correspondence to Zhihong Zhang.

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Zhang, Z., Duan, F., He, L. et al. Electrochemical clenbuterol immunosensor based on a gold electrode modified with zinc sulfide quantum dots and polyaniline. Microchim Acta 183, 1089–1097 (2016). https://doi.org/10.1007/s00604-015-1730-2

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  • DOI: https://doi.org/10.1007/s00604-015-1730-2

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