Skip to main content

Advertisement

SpringerLink
Log in

Silk fibroin/collagen and silk fibroin/chitosan blended three-dimensional scaffolds for tissue engineering

  • Original Article
  • Published:
European Journal of Orthopaedic Surgery & Traumatology Aims and scope Submit manuscript

Abstract

Introduction

In this study, the silk fibroin blended constructs were produced, scaffold performances of different kinds of scaffold were analyzed, and the better type for tissue engineering was optimized.

Methods

The silk fibroin/collagen (SF/C) and silk fibroin/chitosan (SF/CS) were made using a freeze-drying technique, porosity, water absorption expansion rate, mechanical properties and pore size of different scaffold was detected. Bone marrow mesenchymal stem cells (BMSCs) of 4-week-old male Wistar rats were separated by density gradient centrifugation, third generation BMSCs were seeded onto scaffolds, cultured 14 days, proliferation and metabolize of cells were detected in different time using the thiazolyl blue tetrazolium bromide (MTT) assay method, and cell morphology and distribution were observed by histological analysis and scanning electron microscopy (SEM).

Results

Porosity, water absorption expansion rate and Young’s modulus of SF/C were significantly higher than SF/CS (p < 0.05); pore size of SF/C and SF/CS was 103 ± 12 and 76 ± 11 μm and had no significant differences between two types (p > 0.05); MTT results showed that the metabolism of cells in the SF/C was better than SF/CS; after cultivation for 14 days, in the inner zone of scaffolds, cells staining were little or absent from SF/CS, lots of cells staining were existing in SF/C; pore size was consistent, holes communicated with each other better, stem cells grew well inside the scaffolds, extended fully and secreted much extracellular matrix under SEM in SF/C scaffold; internal structure of SF/CS was disorder, holes size were not consistent, and did not communicated with each other and cells were partly dead.

Conclusion

Compared with SF/CS, SF/C scaffold showed better porosity, water absorption expansion rate, elasticity modulus and pore size, cells grow well inside the scaffolds, and was more suitable for tissue engineering.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

Similar content being viewed by others

References

  1. Zhang P, Wang W (2013) Preparation of silk fibroin-chitosan scaffolds and their properties. Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi 27(12):1517–1522

    CAS  PubMed  Google Scholar 

  2. Tiyaboonchai W, Chomchalao P, Pongcharoen S, Sutheerawattananonda M, Sobhon P (2011) Preparation and characterization of blended Bombyx mori silk fibroin scaffolds. Fibers Polym 12:324–333

    Article  CAS  Google Scholar 

  3. Gong X, Liu H, Ding X, Liu M, Li X, Zheng L, Jia X, Zhou G, Zou Y, Li J, Huang X, Fan Y (2014) Physiological pulsatile flow culture conditions to generate functional endothelium on a sulfated silk fibroin nanofibrous scaffold. Biomaterials 35(17):4782–4791

  4. Xu YY, Wu JM, Guan J, Zhang XZ, Li ZH, Wang PF, Li RX, Guo Y, Ning B, Huang SJ (2009) Physio chemical and biological properties of modified collagen sponge from porcine skin. J Wu Han Univ Technol Mater Sci Ed 24(4):619–626

    Article  Google Scholar 

  5. Schuh E, Hofmann S, Stok K, Notbohm H, Müller R, Rotter N (2012) Chondrocyte redifferentiation in 3D. The effect of adhesion site density and substrate elasticity. J Biomed Mater Res A 100A:38–47

    Article  CAS  Google Scholar 

  6. Matmati M, Ng T, Rosenzweig D, Quinn T (2013) Protection of Bovine Chondrocyte Phenotype by Heat Inactivation of Allogeneic Serum in Monolayer Expansion Cultures. Ann Biomed Eng 11(4):1–10

  7. Pan H, Zhang Y, Hang Y, Shao H, Hu X, Xu Y, Feng C (2012) Significantly reinforced composite fibers electrospun from silk fibroin/carbon nanotube aqueous solutions. Biomacromolecules 13(9):2859–2867

    Article  CAS  PubMed  Google Scholar 

  8. Cheon YW, Lee WJ, Baek HS, Lee YD, Park JC, Park YH, Ki CS, Chung KH, Rah DK (2010) Enhanced chondrogenic responses of human articular chondrocytes onto silk fibroin/wool keratose scaffolds treated with microwave-induced argon plasma. Artif Organs 34(5):384–392

    Article  CAS  PubMed  Google Scholar 

  9. Bray Laura J (2012) A dual-layer silk fibroin scaffold for reconstructing the human corneal limbus. Biomaterials 33(13):3529–3538

    Article  CAS  PubMed  Google Scholar 

  10. Cho SY, Heo S, Jin HJ (2012) Controlling microstructure of three-dimensional scaffolds from regenerated silk fibroin by adjusting pH. J Nanosci Nanotechnol 12(1):806–810

    Article  CAS  PubMed  Google Scholar 

  11. Zhang X, Cao C, Ma X, Li Y (2012) Optimization of macroporous 3-D silk fibroin scaffolds by salt-leaching procedure in organic solvent-free conditions. J Mater Sci Mater Med 23(2):315–324

    Article  CAS  PubMed  Google Scholar 

  12. Golinska MD, Wlodarczyk-Biegun MK, Werten MW, Stuart MA, de Wolf FA, de Vries R (2014) Dilute self-healing hydrogels of silk-collagen-like block copolypeptides at neutral pH. Biomacromolecules 15(3):699–706

    Article  CAS  PubMed  Google Scholar 

  13. Shen Y, Redmond SL, Papadimitriou J, Teh BM, Yan S, Wang Y, Atlas MD, Marano RJ, Zheng M, Dilley RJ (2014) The biocompatibility of silk fibroin and acellular collagen scaffolds for tissue engineering in the ear. Biomed Mater 9(1):015015

    Article  PubMed  Google Scholar 

  14. Zeng C, Yang Q, Zhu M, Du L, Zhang J, Ma X, Xu B, Wang L (2014) Silk fibroin porous scaffolds for nucleus pulposus tissue engineering. Mater Sci Eng C Mater Biol Appl 37:232–240

    Article  CAS  PubMed  Google Scholar 

  15. Jin Y, Zhang W, Liu Y, Zhang M, Xu L, Wu Q, Zhang X, Zhu Z, Qingfeng H, Jiang X (2014) rhPDGF-BB via ERK pathway osteogenesis and adipogenesis balancing in ADSCs for critical-size calvarial defect repair. Tissue Eng Part A

  16. Kim UJ, Park J, Joo Kim H, Wada M, Kaplan DL (2005) Three-dimensional aqueous-derived biomaterial scaffolds from silk fibroin. Biomaterials 26:2775–2785

    Article  CAS  PubMed  Google Scholar 

  17. Li X, He J, Bian W, Li Z, Li D, Snedeker JG (2014) A novel silk-TCP-PEEK construct for anterior cruciate ligament reconstruction: an off-the shelf alternative to a bone-tendon-bone autograft. Biofabrication 6(1):015010

    Article  PubMed  Google Scholar 

  18. Ziv K, Nuhn H, Ben-Haim Y, Sasportas LS, Kempen PJ, Niedringhaus TP, Hrynyk M, Sinclair R, Barron AE, Gambhir SS (2014) A tunable silk-alginate hydrogel scaffold for stem cell culture and transplantation. Biomaterials 35(12):3736–3743

    Article  CAS  PubMed  Google Scholar 

  19. Zhao H, Heusler E, Jones G, Li L, Werner V, Germershaus O, Ritzer J, Luehmann T, Meinel L (2014) Decoration of silk fibroin by click chemistry for biomedical application. J Struct Biol 186(3):420–430

  20. Fan Z, Zhang F, Liu T, Zuo BQ (2014) Effect of hyaluronan molecular weight on structure and biocompatibility of silk fibroin/hyaluronan scaffolds. Int J Biol Macromol 65:516–523

    Article  CAS  PubMed  Google Scholar 

  21. Teuschl AH, Neutsch L, Monforte X, Runzler D, van Griensven M, Gabor F, Redl H (2014) Enhanced cell adhesion on silk fibroin via lectin surface modification. Acta Biomater 10(6):2506–2517

  22. Zhu M, Wang K, Mei J, Li C, Zhang J, Zheng W, An D, Xiao N, Zhao Q, Kong D, Wang L (2014) Fabrication of highly interconnected porous silk fibroin scaffolds for potential use as vascular grafts. Acta Biomater 10(5):2014–2023

  23. Zermatten E, Vetsch JR, Ruffoni D, Hofmann S, Muller R, Steinfeld A (2014) Micro-computed tomography based computational fluid dynamics for the determination of shear stresses in scaffolds within a perfusion bioreactor. Ann Biomed Eng 42(5):1085–1094

  24. Yang YJ, Kwon Y, Choi BH, Jung D, Seo JH, Lee KH, Cha HJ (2014) Multifunctional adhesive silk fibroin with blending of RGD-bioconjugated mussel adhesive protein. Biomacromolecules 15(4):1390–1398

  25. Yang C, Lee JS, Jung UW, Seo YK, Park JK, Choi SH (2013) Periodontal regeneration with nano-hydroxyapatite-coated silk scaffolds in dogs. J Periodontal Implant Sci 43(6):315–322

    Article  CAS  PubMed Central  PubMed  Google Scholar 

Download references

Acknowledgments

This research was financially supported by the National Natural Sciences Foundation of China, Nos. 11072266 and 31370942.

Conflict of interest

None.

Author information

Authors and Affiliations

  1. Tianjin Medical University, Qi Xiangtai Road No. 22, Heping District, Tianjin, 300070, China

    Kai Sun, Hui Li, Zhenghao Nian & Dong Li

  2. Institute of Medical Equipment, Academy of Military and Medical Sciences, Wan Dong Road No. 106, Hedong District, Tianjin, 300161, China

    Kai Sun, Ruixin Li, Zhenghao Nian, Dong Li & Cheng Xu

  3. Tianjin Medical University General Hospital, Anshan Road No. 154, Heping District, Tianjin, 300052, China

    Kai Sun, Hui Li, Zhenghao Nian & Dong Li

Authors
  1. Kai Sun
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hui Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ruixin Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Zhenghao Nian
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Dong Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Cheng Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Hui Li.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Sun, K., Li, H., Li, R. et al. Silk fibroin/collagen and silk fibroin/chitosan blended three-dimensional scaffolds for tissue engineering. Eur J Orthop Surg Traumatol 25, 243–249 (2015). https://doi.org/10.1007/s00590-014-1515-z

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00590-014-1515-z

Keywords

Search

Navigation