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Antioxidant and anticholinesterase activity of rutin in aluminum chloride-exposed Drosophila melanogaster

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Abstract

Aluminum (Al) toxicity has been associated with multiple neurodegenerative disorders, including Alzheimer’s disease (AD). Since current medications for AD and Al-related toxicity are reported to have significant side effects, the search for therapeutic agents possessing antioxidant and anticholinesterase potential is ongoing. One such agent is rutin, a flavonoid, reported for its potent antioxidant and anti-inflammatory effects. This study investigated the activity of rutin in mitigating Al chloride (AlCl3) toxicity in Drosophila melanogaster. Flies were divided into six groups containing fifty (50) flies each. Group A served as the control; Group B received 40 mM AlCl3; Groups C and D were co-treated with 40 mM AlCl3 + 0.5 mg/kg rutin and 40 mM AlCl3 + 1 mg/kg, respectively; Groups E and F were treated with rutin alone in doses of 0.5 mg/kg and 1 mg/kg, respectively, all through their diet. Negative geotaxis, superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA), and acetylcholinesterase (AChE) activities were evaluated at the end of the study. A marked decrease (P < 0.05) was noticed in survival rate, negative geotaxis, and SOD as well as a significant increase in MDA and AChE in the AlCl3-exposed flies when compared to the control. Conversely, in the groups co-treated with rutin, there was a significant attenuation of the negative effects of AlCl3. Taken together, these findings suggest that rutin protected against AlCl3, thus indicating the possible therapeutic effects of rutin against aluminum toxicity and its related disorders.

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  1. Department of Anatomy, School of Basic Medical Sciences, University of Benin, Benin City, Edo State, Nigeria

    Mary Ekene Iyiegbu & Adaze Bijou Enogieru

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  1. Mary Ekene Iyiegbu
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Correspondence to Adaze Bijou Enogieru.

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The study was approved by the Research Ethics Committee, College of Medical Sciences, University of Benin, with approval no. CMS/REC/2023/356.

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Iyiegbu, M.E., Enogieru, A.B. Antioxidant and anticholinesterase activity of rutin in aluminum chloride-exposed Drosophila melanogaster. Comp Clin Pathol (2024). https://doi.org/10.1007/s00580-024-03564-8

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