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The impact of aerobic exercise training and crocin consumption on MuRf-1 level in skeletal muscle in high-fat fed ovariectomized rat models

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Abstract

Estrogen deficiency intensifies sarcopenia by activation of inflammatory and oxidative pathways. Aerobic exercise training and antioxidant/inflammatory supplements might reverse such outcomes or decline the degenerative pathway. Thus, we investigated the impact of aerobic training and crocin (CRO) consumption on skeletal muscle MuRf-1 and plasma antioxidant and inflammatory statues in ovariectomized (OVX) rat models fed on a high-fat diet (HFD). 72 female Wistar rats (200 ± 20 gr) were randomly divided into 9 groups: (1) non-OVX + standard chow (nOVX + SC), (2) nOVX + HFD, (3) high-intensity continuous training (HICT) + OVX + HFD + CRO, (4) HICT + OVX + HFD, (5) moderate-intensity continuous training (MICT) + OVX + HFD + CRO, (6) MICT + OVX + HFD, (7) OVX + HFD + CRO, (8) OVX + SC, and (9) OVX + HFD. The OVX rats underwent surgery for menopause model induction. Immediately after the ovariectomy, rats were fed either by HFD or SC. Seven weeks later, exercise training (HICT/MICT; 8-week, 5-session, 12–30 m/min or 12–21 m/min) and CRO consumption (60 mg/kg) intervention per sessions were performed. The plasma levels of SOD, GSH, and TNF-α and muscle levels of MuRf-1 were assessed using the ELISA method. A two-tailed t-test and one-way ANOVA were applied for data analysis. There was not a significant difference in the protein levels of MuRF1 as the CRO caused an increase in the TNF-α levels as compared to the control. HICT significantly decreased SOD levels as compared to the control, CRO, and MICT. There was no significant difference in GSH levels between intervention arms. Exercise training and CRO supplementations did not make any significant changes in muscle mass regulation factors in the OVX rat model.

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Data availability

The data that support the findings of this study are available from the corresponding author upon reasonable request.

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Authors and Affiliations

  1. Department of Exercise Physiology, Faculty of Sports Sciences, University of Mazandaran, Babolsar, Iran

    Shokoufeh Kheradmand & Elahe Talebi-Garakani

  2. Department of Exercise Physiology, Faculty of Physical Education and Sport Sciences, Kharazmi University, Tehran, Iran

    Hamid Rajabi

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  1. Shokoufeh Kheradmand
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  2. Hamid Rajabi
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  3. Elahe Talebi-Garakani
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Contributions

All authors contributed to the study conception and design, material preparation, and data analysis. Sh Kh wrote the first draft of the manuscript, and H R and E T reviewed the manuscript. All authors approved the final version.

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Correspondence to Shokoufeh Kheradmand.

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The article does not contain any studies involving human participants performed by any of the authors. All applicable institutional guidelines for the care and use of animals were approved by the ethical committee of the Science and Research Branch, Mazandaran University (IR.UMZ.REC.1400.026). All experimental procedures were performed on the animals according to the guidelines of the Care and Use of Laboratory Animals by the National Institutes of Health (USA).

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Kheradmand, S., Rajabi, H. & Talebi-Garakani, E. The impact of aerobic exercise training and crocin consumption on MuRf-1 level in skeletal muscle in high-fat fed ovariectomized rat models. Comp Clin Pathol 32, 941–948 (2023). https://doi.org/10.1007/s00580-023-03505-x

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