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Effects of acute experimental Trypanosoma brucei brucei infections on serum super oxide dismutase activities in relations to development of parasitaemia, anaemia and leucopenia in Wistar rats

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Abstract

The alterations in serum super oxide dismutase (SOD) enzymes activities in relation to sequential haematological changes were determined to assess the impacts of oxidative stress in Wistar rats experimentally infected with Trypanosoma brucei brucei. Forty adult male rats allocated into infected and control groups, of 20 rats each based on their mean body weight were used. Rats in infected group were administered 1 ml of reconstituted infected blood containing 1 × 106 trypanosomes/ml obtained from T. b. brucei–infected donor rat. Blood sample in EDTA sample tubes was collected for haematological parameters determinations, and sera were also harvested for determination of SOD activities on day 0 post-infection (pi) (day of infection) and days 3, 5 and 7 pi. Results indicated pre-patent period of 3.75 ± 0.11 days and increase in levels of parasitaemia throughout the study. Packed cell volume (PCV) and total white blood cell count (TWBC) were significantly (p < 0.05) reduced with values of 33.00 ± 2.89% and 6.93 ± 0.48 × 109/L, respectively on day 7 pi compared with control and pre-infection values. Serum SOD activity had a significant (p < 0.05) negative correlation (г = − 0.9) with the levels of parasitaemia in the T. b. brucei–infected rats. In conclusion, infection of rats with T. b. brucei caused a decrease in the serum SOD activities proportional to the level of parasitaemia and duration of the infection, and this is suggested to be responsible for sequential development of anaemia and leucopenia.

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Acknowledgement

Department of Veterinary Pathology, Ahmadu Bello University, Zaria Nigeria

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Authors and Affiliations

  1. Department of Veterinary Pathology, Ahmadu Bello University, Zaria, Nigeria

    Pius J. Erin, Ochuko Orakpoghenor, Joshua T. Adeke, Sani Adamu & King A. N. Esievo

  2. Department of Veterinary Pathology, University of Abuja, Abuja, Nigeria

    Nuhu A. Sani

  3. Department of Veterinary Pathology, University of Ilorin, Ilorin, Nigeria

    Jamila A. Atata

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  1. Pius J. Erin
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  2. Ochuko Orakpoghenor
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  3. Nuhu A. Sani
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Correspondence to Ochuko Orakpoghenor.

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This article contains studies with animal subjects performed by the author and ethical approval for this study was provided by the Ahmadu Bello University Committee on Animal Use and Care (ABUCAUC).

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Erin, P.J., Orakpoghenor, O., Sani, N.A. et al. Effects of acute experimental Trypanosoma brucei brucei infections on serum super oxide dismutase activities in relations to development of parasitaemia, anaemia and leucopenia in Wistar rats. Comp Clin Pathol 29, 311–316 (2020). https://doi.org/10.1007/s00580-019-03066-y

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