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Soil spore bank communities of ectomycorrhizal fungi in endangered Chinese Douglas-fir forests

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Abstract

Chinese Douglas-fir (Pseudotsuga sinensis) is an endangered Pinaceae species found in several isolated regions of China. Although soil spore banks of ectomycorrhizal (ECM) fungi can play an important role in seedling establishment after disturbance, such as in the well-known North American relative (Pseudotsuga menziesii), we have no information about soil spore bank communities in relict forests of Chinese Douglas-fir. We conducted bioassays of 73 soil samples collected from three Chinese Douglas-fir forests, using North American Douglas-fir as bait seedlings, and identified 19 species of ECM fungi. The observed spore bank communities were significantly different from those found in ECM fungi on the roots of resident trees at the same sites (p = 0.02). The levels of potassium (K), nitrogen (N), organic matter, and the pH of soil were the dominant factors shaping spore bank community structure. A new Rhizopogon species was the most dominant species in the spore banks. Specifically, at a site on Sanqing Mountain, 22 of the 57 surviving bioassay seedlings (representing 21 of the 23 soil samples) were colonized by this species. ECM fungal richness significantly affected the growth of bioassay seedlings (R 2 = 0.20, p = 0.007). Growth was significantly improved in seedlings colonized by Rhizopogon or Meliniomyces species compared with uncolonized seedlings. Considering its specificity to Chinese Douglas-fir, predominance in the soil spore banks, and positive effect on host growth, this new Rhizopogon species could play critical roles in seedling establishment and forest regeneration of endangered Chinese Douglas-fir.

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Acknowledgments

This study was supported in part by JSPS KAKENHI Grants (21658054.25660115.15H02449.16H02759) to KN, by the National Natural Science Foundation of China (41571307), the National Key Research and Development Plan (2016YFD0800700), the Science Foundation of Jiangsu Province, China (BE2016812) to YC, by Jiangsu Agriculture Science and Technology Innovation Fund (cx(15)1005) to LH, and by the Science Foundation of Jiangsu Province, China (BK20151301) to JX and (BK20170469) to ZW.

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Authors and Affiliations

  1. Jiangsu Coastal Area Institute of Agricultural Sciences, Yancheng, Jiangsu, 224002, China

    Zhugui Wen, Lizhou Hong & Jincheng Xing

  2. College of Life Sciences, Nanjing Agricultural University, Nanjing, Jiangsu, 210095, China

    Zhugui Wen, Liang Shi, Yangze Tang, Jiawang Xue & Yahua Chen

  3. Graduate School of Frontier Sciences, The University of Tokyo, Kashiwanoha, Kashiwa, Chiba, 277-8563, Japan

    Zhugui Wen & Kazuhide Nara

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  1. Zhugui Wen
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  2. Liang Shi
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  3. Yangze Tang
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  4. Lizhou Hong
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  5. Jiawang Xue
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  6. Jincheng Xing
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  7. Yahua Chen
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Correspondence to Yahua Chen or Kazuhide Nara.

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Wen, Z., Shi, L., Tang, Y. et al. Soil spore bank communities of ectomycorrhizal fungi in endangered Chinese Douglas-fir forests. Mycorrhiza 28, 49–58 (2018). https://doi.org/10.1007/s00572-017-0800-1

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