Abstract
The conceptual simulation study of DNA extraction and separation from salivary fluid sample are mainly divided into two parts. The initial study covered microfluidic channel design and effective DNA extraction by using mobilizing superparamagnetic (SPM) beads in COMSOL Multiphysics® software. The subsequent part presented simulation work for DNA separation from SPM beads in electromagnetization field. The main objective of this study in microfluidic platform in presence of electromagnetic field was to achieve higher and uncontaminated yield of DNA as sample preparation step which can be applied for clinical analysis. COMSOL Multiphysics® simulation software was used to study the entire work and presented in this paper with complete study steps. The attached DNA onto SPM beads was separated by specifying computational fluid dynamics in laminar flow, particle tracing module and electromagnetic field by using external electromagnetic coil as magnet array. Most of the SPM beads were captured and held by magnetic field when 100 mT/min was generated by electromagnetic coil. 82.01% of uncontaminated DNA yield from salivary fluid was able to separate from SPM beads after DNA extraction in microfluidic channel. In the absence of external magnetic field, the produced superparamagnetism enable SPM beads to flip in an isotropy provide efficient separation. The mechanical and chemical stability of silica surface of SPM beads provide an excellent chromatography separation medium for DNA sample preparation for further downstream analysis.
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Acknowledgements
This research was supported by the Ministry of Higher Education Malaysia (MoHE) under the Fundamental Research Grant Scheme (FRGS/1/2016/TK04/UKM/02/5).
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Gauri, S., Gan, K.B. & Then, SM. Simulation of DNA extraction and separation from salivary fluid by superparamagnetic beads and electromagnetic field in microfluidic platform. Microsyst Technol 25, 1379–1385 (2019). https://doi.org/10.1007/s00542-018-4102-0
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DOI: https://doi.org/10.1007/s00542-018-4102-0