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Biotransformation of lysine into cadaverine using barium alginate-immobilized Escherichia coli overexpressing CadA

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Abstract

In this study, Escherichia coli cells overexpressing lysine decarboxylase (CadA) were used for cadaverine production. Barium alginate was selected as a matrix for immobilization of E. coli YH91. Free cells and immobilized cells (IC) were characterized for their physiochemical properties, and the optimum pH and temperature were determined as 6 and 37 °C, respectively. Immobilized cells were three times more thermally stable compared to free cells at the optimum temperature and had a half-life (t 1/2) of 131 h. The free cells lost most of lysine decarboxylase activity after nine cycles, but in contrast immobilized cells retained 56 % of their residual activity even after the 18th cycle. The immobilized cells gave a maximum production of cadaverine (75.8 g/L) with 84 % conversion.

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Acknowledgments

The study was partially supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Education (NRF-2013R1A1A2A10004690), (NRF-2015R1A2A2A04006014) and (NRF-2015M1A5A1037196). This research was also supported by the Bio & Medical Technology Development Program of the NRF funded by the Korean government, MISP (2015M3A9B8031831) and by the R&D Program of MOTIE/KEIT [10047910, Production of biobased cadaverine and polymerization of Bio-polyamide 510]. This research was also supported by the 2015 KU Brain Pool Program of Konkuk University.

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Authors and Affiliations

  1. Department of Biological Engineering, College of Engineering, Konkuk University, Seoul, 143-701, Republic of Korea

    Shashi Kant Bhatia, Yong Hyun Kim, Hyun Joong Kim, Hyung-Min Seo, Jung-Ho Kim, Hun-Seok Song, Ganesan Sathiyanarayanan & Yung-Hun Yang

  2. Food Ingredients Center, Foods R&D, CheilJedang, Guro-dong, Guro-Gu, Seoul, 152-051, Republic of Korea

    Sung-Hee Park

  3. Department of Biological and Chemical Engineering, Hongik University, Sejong Ro 2639, Jochiwon, Sejong, 339-701, Republic of Korea

    Kyungmoon Park

  4. Microbial Carbohydrate Resource Bank, Konkuk University, Seoul, 143-701, Republic of Korea

    Yung-Hun Yang

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  1. Shashi Kant Bhatia
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  2. Yong Hyun Kim
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  3. Hyun Joong Kim
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  4. Hyung-Min Seo
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  5. Jung-Ho Kim
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  6. Hun-Seok Song
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  7. Ganesan Sathiyanarayanan
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  8. Sung-Hee Park
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Correspondence to Yung-Hun Yang.

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Bhatia, S.K., Kim, Y.H., Kim, H.J. et al. Biotransformation of lysine into cadaverine using barium alginate-immobilized Escherichia coli overexpressing CadA. Bioprocess Biosyst Eng 38, 2315–2322 (2015). https://doi.org/10.1007/s00449-015-1465-9

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  • DOI: https://doi.org/10.1007/s00449-015-1465-9

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