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Immobilization of halophilic Bacillus sp. EMB9 protease on functionalized silica nanoparticles and application in whey protein hydrolysis

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Abstract

The present work targets the fabrication of an active, stable, reusable enzyme preparation using functionalized silica nanoparticles as an effective enzyme support for crude halophilic Bacillus sp. EMB9 protease. The immobilization efficiency under optimized conditions was 60 %. Characterization of the immobilized preparation revealed marked increase in pH and thermal stability. It retained 80 % of its original activity at 70 °C while t 1/2 at 50 °C showed a five-fold enhancement over that for the free protease. Kinetic constants K m and V max were indicative of a higher reaction velocity along with decreased affinity for substrate. The preparation could be efficiently reused up to 6 times and successfully hydrolysed whey proteins with high degree of hydrolysis. Immobilization of a crude halophilic protease on a nanobased scaffold makes the process cost effective and simple.

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Acknowledgments

The financial support provided by the Department of Biotechnology (Government of India) is gratefully acknowledged. Author Rajeshwari Sinha is grateful to the Council of Scientific and Industrial Research (CSIR), Government of India, for providing the Research Fellowship.

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  1. Enzyme and Microbial Biochemistry Laboratory, Department of Chemistry, Indian Institute of Technology, Hauz Khas, New Delhi, 110016, India

    Rajeshwari Sinha & S. K. Khare

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  1. Rajeshwari Sinha
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  2. S. K. Khare
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Correspondence to S. K. Khare.

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Sinha, R., Khare, S.K. Immobilization of halophilic Bacillus sp. EMB9 protease on functionalized silica nanoparticles and application in whey protein hydrolysis. Bioprocess Biosyst Eng 38, 739–748 (2015). https://doi.org/10.1007/s00449-014-1314-2

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  • DOI: https://doi.org/10.1007/s00449-014-1314-2

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