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Improvement of degummed ramie fiber properties upon treatment with cellulase secreting immobilized A. larrymoorei A1

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Abstract

Degummed ramie fiber is widely used in the textile industry. Cellulase enzyme can be effectively used for bio-polishing of the ramie fiber. We immobilized Agrobacterium larrymoorei A1, a potent extra-cellular cellulase producing bacteria, in Ca-alginate. The production of enzyme significantly increased with increasing alginate concentration and reached a maximum activity of 0.28 IU/ml at 20 g/l, which was 32 % higher as compared to free cells. These immobilized cells were used on ramie fibers. Scanning electron micrograph (SEM) and differential interference contrast (DIC) studies showed increased smoothness and orientation of surface structure of the fibers after 19.5 h. The single fiber tenacity was almost same as compared to non-treated fiber and the initial modulus increased by 24.01 %. The remarkable reusability of these immobilized cells provides a cost effective method for treatment of natural fibers containing cellulose.

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Acknowledgments

We thank CAS (UGC), DST-FIST for providing some of the instrument facilities at the Department of Biochemistry, University of Calcutta. AB was supported by doctoral fellowship from UGC-SAP-RFSMS scheme. AG is supported by Ramanujan Fellowship from Department of Science and Technology, India (SR/S2/RJN-106/2012).

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Author notes

  1. Amit Bera

    Present address: Department of Medicine, Division of Nephrology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX, 78229 3900, USA

Authors and Affiliations

  1. Department of Biochemistry, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700019, West Bengal, India

    Amit Bera, Dhrubajyoti Chattopadhyay & Krishanu Chakrabarti

  2. Department of Biochemistry, Bose Institute, Centenary Campus, P 1/12, C.I.T Road, Scheme VIIM, Kolkata, 700054, West Bengal, India

    Abhrajyoti Ghosh

  3. Department of Jute and Fiber Technology, Institute of Jute Technology, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700019, West Bengal, India

    Asis Mukhopadhyay

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  1. Amit Bera
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Correspondence to Dhrubajyoti Chattopadhyay or Krishanu Chakrabarti.

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Bera, A., Ghosh, A., Mukhopadhyay, A. et al. Improvement of degummed ramie fiber properties upon treatment with cellulase secreting immobilized A. larrymoorei A1. Bioprocess Biosyst Eng 38, 341–351 (2015). https://doi.org/10.1007/s00449-014-1274-6

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