Abstract
Pannexin1 (Panx1) is one of three members of the pannexin protein family. The expression of Panx1 mRNA has been extensively investigated from late embryonic to adult stages. In contrast, expression during early embryonic development is largely unknown. Our aim is to examine the temporal and spatial expression of Panx1 in mouse embryonic development by focusing on embryonic days (E) 9.5 to 12.5. Whole embryos are investigated in order to provide a comprehensive survey. Analyses were performed at the mRNA level by using reverse transcription plus the polymerase chain reaction and whole-mount in situ hybridization. Panx1 mRNA was detected in the heads and bodies of embryos at all developmental stages investigated (E9.5, E10.5, E11.5, E12.5). In particular, the nervous system expressed Panx1 at an early time point. Interestingly, Panx1 expression was found in afferent ganglia of the cranial nerves and spinal cord. This finding is of particular interest in the context of neuropathic pain and other Panx1-related neurological disorders. Our study shows, for the first time, that Panx1 is expressed in the central and peripheral nervous system during early developmental stages. The consequences of Panx1 deficiency or inhibition in a number of experimental paradigms might therefore be predicated on changes during early development.
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Acknowledgment
The authors wish to thank Prof. Dr. Georg Zoidl (Bochum, Germany; Canada) for the gift of the mPanx1 plasmid. They are also very grateful to Prof. Dr. Beate Brand-Saberi, Dr. Verena Chankiewicz and Swantje Wulf for their expert advice on whole-mount in situ hybridization techniques, to Prof. Dr. Hannah Monyer for providing Panx1 transgenic mice and to campus colleagues Prof. Dr. Uwe Walldorf for his support and the opportunity to use his microscopic equipment, Dr. Barbara Schäfer for support with the cloning of DNA fragments and Ann Soether for language editing. The data and findings of this study were obtained from the dissertation of Abdulrahman Raslan.
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Raslan, A., Hainz, N., Beckmann, A. et al. Pannexin-1 expression in developing mouse nervous system: new evidence for expression in sensory ganglia. Cell Tissue Res 364, 29–41 (2016). https://doi.org/10.1007/s00441-015-2294-5
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DOI: https://doi.org/10.1007/s00441-015-2294-5