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Expression and localization of SerpinB11 in mouse uteri during peri-implantation and the estrous cycle

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Abstract

Serine protease inhibitor (Serpin) B11 has been identified as a novel serine protease inhibitor but the biological functions of SerpinB11 in female reproduction are unknown. Therefore, we investigate the spatiotemporal expression and regulation of SerpinB11 during the peri-implantation period. SerpinB11 mRNA and protein were detected in the uteri of pregnant mice on days 1–8 (day 1 = presence of a vaginal plug). SerpinB11 protein was localized in the embryonic implantation site on day 5 when embryo implantation occurred and was also strongly expressed in the primary decidual zone on day 6 and secondary decidual zone on days 7 and 8. The expression of SerpinB11 was induced by the activated blastocyst (based on patterns of expression during pseudopregnancy and delayed implantation) and by artificially induced decidualization. Moreover, expression of SerpinB11 was regulated by estradiol and progesterone in ovariectomized mice. The results were further supported by data from the estrous cycle. Thus, SerpinB11 is probably involved in embryo implantation and decidualization.

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Authors and Affiliations

  1. Key Laboratory of Fertility Preservation and Maintenance of Ministry of Education, Key Laboratory of Reproduction and Genetics in Ningxia, Department of Histology and Embryology, Ningxia Medical University, Yinchuan, Ningxia, 75004, People’s Republic of China

    Yanzhou Yang, Huiming Ma, Wenzhi Ma, Miao Sun, Jing Pu, Jia Yu, Ning Zhang, Xiuqin Cao, Xiuying Pei & Yanrong Wang

Authors
  1. Yanzhou Yang
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  2. Huiming Ma
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  3. Wenzhi Ma
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  4. Miao Sun
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  5. Jing Pu
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  6. Jia Yu
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  7. Ning Zhang
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  8. Xiuqin Cao
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  9. Xiuying Pei
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  10. Yanrong Wang
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Corresponding authors

Correspondence to Xiuying Pei or Yanrong Wang.

Additional information

This work was supported by Ningxia Medical University Project (XM2012026) and the Natural Science Foundation of Ningxia (NZ13270).

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Yang, Y., Ma, H., Ma, W. et al. Expression and localization of SerpinB11 in mouse uteri during peri-implantation and the estrous cycle. Cell Tissue Res 357, 373–380 (2014). https://doi.org/10.1007/s00441-014-1829-5

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  • DOI: https://doi.org/10.1007/s00441-014-1829-5

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