Skip to main content
SpringerLink
Log in

Genomic diversity and population structure of three autochthonous Greek sheep breeds assessed with genome-wide DNA arrays

  • Original Article
  • Published:
Molecular Genetics and Genomics Aims and scope Submit manuscript

Abstract

In the present study, genome-wide genotyping was applied to characterize the genetic diversity and population structure of three autochthonous Greek breeds: Boutsko, Karagouniko and Chios. Dairy sheep are among the most significant livestock species in Greece numbering approximately 9 million animals which are characterized by large phenotypic variation and reared under various farming systems. A total of 96 animals were genotyped with the Illumina’s OvineSNP50K microarray beadchip, to study the population structure of the breeds and develop a specialized panel of single-nucleotide polymorphisms (SNPs), which could distinguish one breed from the others. Quality control on the dataset resulted in 46,125 SNPs, which were used to evaluate the genetic structure of the breeds. Population structure was assessed through principal component analysis (PCA) and admixture analysis, whereas inbreeding was estimated based on runs of homozygosity (ROHs) coefficients, genomic relationship matrix inbreeding coefficients (FGRM) and patterns of linkage disequilibrium (LD). Associations between SNPs and breeds were analyzed with different inheritance models, to identify SNPs that distinguish among the breeds. Results showed high levels of genetic heterogeneity in the three breeds. Genetic distances among breeds were modest, despite their different ancestries. Chios and Karagouniko breeds were more genetically related to each other compared to Boutsko. Analysis revealed 3802 candidate SNPs that can be used to identify two-breed crosses and purebred animals. The present study provides, for the first time, data on the genetic background of three Greek indigenous dairy sheep breeds as well as a specialized marker panel that can be applied for traceability purposes as well as targeted genetic improvement schemes and conservation programs.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

Similar content being viewed by others

References

  • Alexander DH, Lange K (2011) Enhancements to the ADMIXTURE algorithm for individual ancestry estimation. BMC Bioinf 12:246

    Article  Google Scholar 

  • Al-Mamun HA, Clark SA, Kwan P, Gondro C (2015) Genome-wide linkage disequilibrium and genetic diversity in five populations of Australian domestic sheep. Genetics Sel Evolut 47:90

    Article  Google Scholar 

  • Anagnostopoulos AK, Katsafadou AI, Pierros V, Kontopodis E, Fthenakis GC, Arsenos G, Karkabounas SC, Tzora A, Skoufos I, Tsangaris GT (2016) Milk of Greek sheep and goat breeds; characterization by means of proteomics. J Proteomics 147:76–84

    Article  CAS  PubMed  Google Scholar 

  • Banos G, Bramis G, Bush SJ, Clark EL, McCulloch MEB, Smith J, Schulze G, Arsenos G, Hume DA, Psifidi A (2017) The genomic architecture of mastitis resistance in dairy sheep. BMC Genomics 18:624

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Barbato M, Orozco-TerWengel P, Tapio M, Bruford MW (2015) SNeP: a tool to estimate trends in recent effective population size trajectories using genome-wide SNP data. Frontiers in Genetics 6

  • Barbato M, Hailer F, Orozco-terWengel P, Kijas J, Mereu P, Cabras P, Mazza R, Pirastru M, Bruford MW (2017) Genomic signatures of adaptive introgression from European mouflon into domestic sheep. Sci Rep 7:7623

    Article  PubMed  PubMed Central  Google Scholar 

  • Brito LF, McEwan JC, Miller SP, Pickering NK, Bain WE, Dodds KG, Schenkel FS, Clarke SM (2017) Genetic diversity of a New Zealand multi-breed sheep population and composite breeds’ history revealed by a high-density SNP chip. BMC Genetics 18:25

    Article  PubMed  PubMed Central  Google Scholar 

  • Buchmann R, Hazelhurst S (2015) The ‘genesis’ manual. University of the Witwatersrand, Johannesburg

    Google Scholar 

  • Chitneedi PK, Arranz JJ, Suarez-Vega A, Garcia-Gamez E, Gutierrez-Gil B (2017) Estimations of linkage disequilibrium, effective population size and ROH-based inbreeding coefficients in Spanish Churra sheep using imputed high-density SNP genotypes. Anim Genet 48:436–446

    Article  CAS  PubMed  Google Scholar 

  • Ciani E, Crepaldi P, Nicoloso L, Lasagna E, Sarti FM, Moioli B, Napolitano F, Carta A, Usai G, D’Andrea M, Marletta D, Ciampolini R, Riggio V, Occidente M, Matassino D, Kompan D, Modesto P, Macciotta N, Ajmone-Marsan P, Pilla F (2014) Genome-wide analysis of Italian sheep diversity reveals a strong geographic pattern and cryptic relationships between breeds. Anim Genet 45:256–266

    Article  CAS  PubMed  Google Scholar 

  • Clarke SM, Henry HM, Dodds KG, Jowett TWD, Manley TR, Anderson RM, McEwan JC (2014) A high throughput single nucleotide polymorphism multiplex assay for parentage assignment in New Zealand sheep. Plos One 9:e93392

    Article  PubMed  PubMed Central  Google Scholar 

  • Corbin LJ, Liu AYH, Bishop SC, Woolliams JA (2012) Estimation of historical effective population size using linkage disequilibria with marker data. J Anim Breed Genet 129:257–270

    Article  CAS  PubMed  Google Scholar 

  • Dalvit C, De Marchi M, Dal Zotto R, Zanetti E, Meuwissen T, Cassandro M (2008) Genetic characterization of the Burlina cattle breed using microsatellites markers. J Anim Breed Genet 125:137–144

    Article  CAS  PubMed  Google Scholar 

  • Dimauro C, Cellesi M, Steri R, Gaspa G, Sorbolini S, Stella A, Macciotta NPP (2013) Use of the canonical discriminant analysis to select SNP markers for bovine breed assignment and traceability purposes. Anim Genet 44:377–382

    Article  CAS  PubMed  Google Scholar 

  • Dodds KG, Auvray B, Newman SAN, McEwan JC (2014) Genomic breed prediction in New Zealand sheep. BMC Genetics 15:92

    Article  PubMed  PubMed Central  Google Scholar 

  • Excoffier L, Lischer HEL (2010) Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Resour 10:564–567

    Article  PubMed  Google Scholar 

  • Fan B, Du ZQ, Gorbach DM, Rothschild MF (2010) Development and application of high-density SNP arrays in genomic studies of domestic animals. Asian-Aust J Anim Sci 23:833–847

    Article  CAS  Google Scholar 

  • FAO (1998) Secondary guidelines for development of national farm animal genetic resources management plans. Management of Small Populations at Risk, Rome

    Google Scholar 

  • FAOSTAT (2016) Food and Agriculture Organization of the United Nations. FAOSTAT Database. Rome, Italy: FAO. Retrieved November 18, 2016 from http://www.fao.org/faostat/en/#data/QL

  • Felius M, Theunissen B, Lenstra JA (2015) Conservation of cattle genetic resources: the role of breeds. J Agric Sci 153:152–162

    Article  Google Scholar 

  • Fernandez ME, Goszczynski DE, Liron JP, Villegas-Castagnasso EE, Carino MH, Ripoli MV, Rogberg-Munoz A, Posik DM, Peral-Garcia P, Giovambattista G (2013) Comparison of the effectiveness of microsatellites and SNP panels for genetic identification, traceability and assessment of parentage in an inbred Angus herd. Genet Mol Biol 36:185–191

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Fontanesi L, Beretti F, Dall’Olio S, Portolano B, Matassino D, Russo V (2011) A melanocortin 1 receptor (MC1R) gene polymorphism is useful for authentication of Massese sheep dairy products. J Dairy Res 78:122–128

    Article  CAS  PubMed  Google Scholar 

  • Gaouar SBS, Lafri M, Djaout A, El-Bouyahiaoui R, Bouri A, Bouchatal A, Maftah A, Ciani E, Da Silva AB (2017) Genome-wide analysis highlights genetic dilution in Algerian sheep. Heredity 118:293–301

    Article  CAS  PubMed  Google Scholar 

  • Garke C, Ytournel F, Bed’hom B, Gut I, Lathrop M, Weigend S, Simianer H (2012) Comparison of SNPs and microsatellites for assessing the genetic structure of chicken populations. Anim Genet 43:419–428

    Article  CAS  PubMed  Google Scholar 

  • Gelasakis AI, Valergakis GE, Arsenos G, Banos G (2012) Description and typology of intensive Chios dairy sheep farms in Greece. J Dairy Sci 95:3070–3079

    Article  CAS  PubMed  Google Scholar 

  • Gonzalez JR, Armengol L, Sole X, Guino E, Mercader JM, Estivill X, Moreno V (2007) SNPassoc: an R package to perform whole genome association studies. Bioinformatics 23:644–645

    PubMed  Google Scholar 

  • Grossi DA, Jafarikia M, Brito LF, Buzanskas ME, Sargolzaei M, Schenkel FS (2017) Genetic diversity, extent of linkage disequilibrium and persistence of gametic phase in Canadian pigs. Bmc Genetics 18:6

    Article  PubMed  PubMed Central  Google Scholar 

  • Guichoux E, Lagache L, Wagner S, Chaumeil P, Leger P, Lepais O, Lepoittevin C, Malausa T, Revardel E, Salin F, Petit RJ (2011) Current trends in microsatellite genotyping. Mol Ecol Resour 11:591–611

    Article  CAS  PubMed  Google Scholar 

  • Hatziminaoglou I (2006a) Sheep and goats in Greece and in the world. Ed. Jahoudi, Thessaloniki Greece. 2nd edn

  • Hatziminaoglou I (2006b) Sheep and goats in Greece and worldwide. Yahoudi-Yapouli Press, Thessaloniki

    Google Scholar 

  • Hatziminaoglou I, Liamadis D, Avdi M (2006) Introduction to animal production. Yahoudi-Yapouli Press, Thessaloniki

    Google Scholar 

  • Hatziminaoglu J, Zervas NP, Boyazoglu J (1990) Prolific dairy sheep breeds in Greece. In: Bougler J (ed.), Tisserand J-L (eds). Les petits ruminants et leurs productions laitières dans la region méditerranéenne.. Montpellier: CIHEAM. pp 25–30 (Options Méditerranéennes: Série A.Séminaires Méditerranéens; n. 12)

  • Hayes B, Goddard M (2010) Genome-wide association and genomic selection in animal breeding. Genome 53:876–883

    Article  CAS  PubMed  Google Scholar 

  • Heaton MP, Leymaster KA, Kalbfleisch TS, Kijas JW, Clarke SM, McEwan J, Maddox JF, Basnayake V, Petrik DT, Simpson B, Smith TPL, Chitko-McKown CG, Consortium ISG (2014) SNPs for parentage testing and traceability in globally diverse breeds of sheep. Plos One 9

  • Howrigan DP, Simonson MA, Keller MC (2011) Detecting autozygosity through runs of homozygosity: a comparison of three autozygosity detection algorithms. BMC Genomics 12:460

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ishibashi K, Hara S, Kondo S (2009) Aquaporin water channels in mammals. Clin Exp Nephrol 13:107–117

    Article  CAS  PubMed  Google Scholar 

  • Keller MC, Visscher PM, Goddard ME (2011) Quantification of inbreeding due to distant ancestors and its detection using dense single nucleotide polymorphism data. Genetics 189:237–249

    Article  PubMed  PubMed Central  Google Scholar 

  • Khatkar MS, Nicholas FW, Collins AR, Zenger KR, Al Cavanagh J, Barris W, Schnabel RD, Taylor JF, Raadsma HW (2008) Extent of genome-wide linkage disequilibrium in Australian Holstein-Friesian cattle based on a high-density SNP panel. BMC Genomics 9:187

    Article  PubMed  PubMed Central  Google Scholar 

  • Kijas JW, Lenstra JA, Hayes B, Boitard S, Neto LRP, San Cristobal M, Servin B, McCulloch R, Whan V, Gietzen K, Paiva S, Barendse W, Ciani E, Raadsma H, McEwan J, Dalrymple B, Consortium ISG (2012) Genome-wide analysis of the world’s sheep breeds reveals high levels of historic mixture and strong recent selection. Plos Biol 10:e1001258

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kominakis A, Hager-Theodorides AL, Zoidis E, Saridaki A, Antonakos G, Tsiamis G (2017) Combined GWAS and ‘guilt by association’-based prioritization analysis identifies functional candidate genes for body size in sheep. Genetics Sel Evol 49:41

    Article  Google Scholar 

  • Ku CS, Naidoo N, Teo SM, Pawitan Y (2011) Regions of homozygosity and their impact on complex diseases and traits. Hum Genet 129:1–15

    Article  PubMed  Google Scholar 

  • Lenstra JA, Groeneveld LF, Eding H, Kantanen J, Williams JL, Taberlet P, Nicolazzi EL, Solkner J, Simianer H, Ciani E, Garcia JF, Bruford MW, Ajmone-Marsan P, Weigend S (2012) Molecular tools and analytical approaches for the characterization of farm animal genetic diversity. Anim Genet 43:483–502

    Article  CAS  PubMed  Google Scholar 

  • Leroy G (2014) Inbreeding depression in livestock species: review and meta-analysis. Anim Genet 45:618–628

    Article  CAS  PubMed  Google Scholar 

  • Ligda C, Altarayrah J, Georgoudis A, Consortium E (2009) Genetic analysis of Greek sheep breeds using microsatellite markers for setting conservation priorities. Small Rumin Res 83:42–48

    Article  Google Scholar 

  • Loukovitis D, Siasiou A, Mitsopoulos I, Lymberopoulos AG, Laga V, Chatziplis D (2016) Genetic diversity of Greek sheep breeds and transhumant populations utilizing microsatellite markers. Small Rumin Res 136:238–242

    Article  Google Scholar 

  • Lu D, Sargolzaei M, Kelly M, Li C, Vander Voort G, Wang Z, Plastow G, Moore S, Miller SP (2012) Linkage disequilibrium in Angus, Charolais, and Crossbred beef cattle. Front Genet 3:152

    Article  PubMed  PubMed Central  Google Scholar 

  • Mastrangelo S, Di Gerlando R, Tolone M, Tortorici L, Sardina MT, Portolano B, International Sheep Genomics C (2014) Genome wide linkage disequilibrium and genetic structure in Sicilian dairy sheep breeds. BMC Genet 15:108

    Article  PubMed  PubMed Central  Google Scholar 

  • Mastrangelo S, Portolano B, Di Gerlando R, Ciampolini R, Tolone M, Sardina MT, Con ISG (2017) Genome-wide analysis in endangered populations: a case study in Barbaresca sheep. Animal 11:1107–1116

    Article  CAS  PubMed  Google Scholar 

  • McLaren W, Gil L, Hunt SE, Riat HS, Ritchie GR, Thormann A, Flicek P, Cunningham F (2016) The Ensembl Variant Effect Predictor. Genome Biol 17:122

    Article  PubMed  PubMed Central  Google Scholar 

  • McQuillan R, Leutenegger AL, Abdel-Rahman R, Franklin CS, Pericic M, Barac-Lauc L, Smolej-Narancic N, Janicijevic B, Polasek O, Tenesa A, MacLeod AK, Farrington SM, Rudan P, Hayward C, Vitart V, Rudan I, Wild SH, Dunlop MG, Wright AF, Campbell H, Wilson JF (2008) Runs of homozygosity in European populations. Am J Hum Genet 83:359–372

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Meadows JRS, Chan EKF, Kijas JW (2008) Linkage disequilibrium compared between five populations of domestic sheep. Bmc Genetics 9:61

    Article  PubMed  PubMed Central  Google Scholar 

  • Miller JM, Poissant J, Kijas JW, Coltman DW, Consortium ISG (2011) A genome-wide set of SNPs detects population substructure and long range linkage disequilibrium in wild sheep. Mol Ecol Resour 11:314–322

    Article  CAS  PubMed  Google Scholar 

  • Mobasheri A, Barrett-Jolley R (2014) Aquaporin water channels in the mammary gland: from physiology to pathophysiology and neoplasia. J Mammary Gland Biol Neoplasia 19:91–102

    Article  PubMed  Google Scholar 

  • Murtagh F, Legendre P (2014) Ward’s hierarchical agglomerative clustering method: which algorithms implement ward’s criterion? J Classif 31:274–295

    Article  Google Scholar 

  • Nicolazzi EL, Caprera A, Nazzicari N, Cozzi P, Strozzi F, Lawley C, Pirani A, Soans C, Brew F, Jorjani H, Evans G, Simpson B, Tosser-Klopp G, Brauning R, Williams JL, Stella A (2015) SNPchiMp v.3: integrating and standardizing single nucleotide polymorphism data for livestock species. BMC Genom 16:283

    Article  Google Scholar 

  • Niu LL, Li HB, Ma YH, Du LX (2012) Genetic variability and individual assignment of Chinese indigenous sheep populations (Ovis aries) using microsatellites. Anim Genet 43:108–111

    Article  CAS  PubMed  Google Scholar 

  • Pariset L, Cuteri A, Ligda C, Ajmone-Marsan P, Valentini A, Consortium E (2009) Geographical patterning of sixteen goat breeds from Italy, Albania and Greece assessed by Single Nucleotide Polymorphisms. BMC Ecol 9:20

    Article  PubMed  PubMed Central  Google Scholar 

  • Pariset L, Mariotti M, Gargani M, Joost S, Negrini R, Perez T, Bruford M, Marsan PA, Valentini A (2011) Genetic diversity of sheep breeds from Albania, Greece, and Italy assessed by mitochondrial DNA and nuclear polymorphisms (SNPs). Sci World J 11:1641–1659

    Article  Google Scholar 

  • Peter C, Bruford M, Perez T, Dalamitra S, Hewitt G, Erhardt G (2007) Genetic diversity and subdivision of 57 European and Middle-Eastern sheep breeds. Anim Genet 38:37–44

    Article  CAS  PubMed  Google Scholar 

  • Pritchard JK, Przeworski M (2001) Linkage disequilibrium in humans: models and data. Am J Hum Genet 69:1–14

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Purcell S, Neale B, Todd-Brown K, Thomas L, Ferreira MAR, Bender D, Maller J, Sklar P, de Bakker PIW, Daly MJ, Sham PC (2007) PLINK: a tool set for whole-genome association and population-based linkage analyses. Am J Hum Genet 81:559–575

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Purfield DC, McParland S, Wall E, Berry DP (2017) The distribution of runs of homozygosity and selection signatures in six commercial meat sheep breeds. PLoS One 12:e0176780

    Article  PubMed  PubMed Central  Google Scholar 

  • R Development Core Team (2010) R: a language and environment for statistical computing. In. R Foundation for Statistical Computing, Vienna, Austria

    Google Scholar 

  • Raadsma HW, Thomson PC, Zenger KR, Cavanagh C, Lam MK, Jonas E, Jones M, Attard G, Palmer D, Nicholas FW (2009) Mapping quantitative trait loci (QTL) in sheep. I. A new male framework linkage map and QTL for growth rate and body weight. Genet Sel Evol 41:34

    Article  PubMed  PubMed Central  Google Scholar 

  • Ramos AM, Megens HJ, Crooijmans RPMA., Schook LB, Groenen MAM (2011) Identification of high utility SNPs for population assignment and traceability purposes in the pig using high-throughput sequencing. Anim Genet 42:613–620

    Article  CAS  PubMed  Google Scholar 

  • Sardina MT, Tortorici L, Mastrangelo S, Di Gerlando R, Tolone M, Portolano B (2015) Application of microsatellite markers as potential tools for traceability of Girgentana goat breed dairy products. Food Res Int 74:115–122

    Article  CAS  PubMed  Google Scholar 

  • Schaffner SF (2004) The X chromosome in population genetics. Nat Rev Genet 5:43–51

    Article  CAS  PubMed  Google Scholar 

  • Tanaka Y, Morishita Y, Ishibashi K (2015) Aquaporin10 is a pseudogene in cattle and their relatives. Biochem Biophys Rep 1:16–21

    PubMed  PubMed Central  Google Scholar 

  • Valergakis GE, Gelasakis AI, Oikonomou G, Arsenos G, Fortomaris P, Banos G (2010) Profitability of a dairy sheep genetic improvement program using artificial insemination. Animal 4:1628–1633

    Article  CAS  PubMed  Google Scholar 

  • VanRaden PM (2008) Efficient methods to compute genomic predictions. J Dairy Sci 91:4414–4423

    Article  CAS  PubMed  Google Scholar 

  • Warnes GR, Bolker B, Bonebakker L, Gentleman R, Liaw WHA, Lumley T, Maechler M, Magnusson A, Moeller S, Schwartz M, Venables B (2011) gplots: various R programming tools for plotting data. R Foundation for Statistical Computing, Vienna. http://CRAN.R-project.org/package=gplot

  • Yang JA, Lee SH, Goddard ME, Visscher PM (2011) GCTA: a tool for genome-wide complex trait analysis. Am J Hum Genet 88:76–82

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zanella R, Peixoto JO, Cardoso FF, Cardoso LL, Biegelmeyer P, Cantao ME, Otaviano A, Freitas MS, Caetano AR, Ledur MC (2016) Genetic diversity analysis of two commercial breeds of pigs using genomic and pedigree data. Genetics Sel Evol 48:24

    Article  Google Scholar 

  • Zdobnov EM, Tegenfeldt F, Kuznetsov D, Waterhouse RM, Simao FA, Ioannidis P, Seppey M, Loetscher A, Kriventseva EV (2017) OrthoDB v9.1: cataloging evolutionary and functional annotations for animal, fungal, plant, archaeal, bacterial and viral orthologs. Nucleic Acids Res 45:D744-D749

    Article  PubMed  Google Scholar 

  • Zeder MA (2008) Domestication and early agriculture in the Mediterranean Basin: Origins, diffusion, and impact. Proc Natl Acad Sci USA 105:11597–11604

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Zhang QQ, Calus MPL, Guldbrandtsen B, Lund MS, Sahana G (2015) Estimation of inbreeding using pedigree, 50 k SNP chip genotypes and full sequence data in three cattle breeds. Bmc Genetics 16:88

    Article  PubMed  PubMed Central  Google Scholar 

  • Zhao FP, Wang GK, Zeng T, Wei CH, Zhang L, Wang HH, Zhang SZ, Liu RZ, Liu Z, Du LX (2014) Estimations of genomic linkage disequilibrium and effective population sizes in three sheep populations. Livestock Sci 170:22–29

    Article  Google Scholar 

Download references

Acknowledgements

This work was partially supported by the program ‘GOSHOMICS’ under the action COOPERATION 2009 (SYNERGASIA 2009), financed by the European Regional Development Fund and national resources, project code: 09SYN-23-990.

Author information

Authors and Affiliations

  1. Laboratory of Animal Husbandry, School of Veterinary Medicine, Department of Animal Production, Ichthyology, Ecology and Environmental Protection, Aristotle University of Thessaloniki, Thessaloniki, Greece

    S. Michailidou, G. Banos & G. Arsenos

  2. Institute of Applied Biosciences, Center for Research and Technology Hellas, 6th Km Charilaou Thermi Road, 57001, Thermi, Greece

    S. Michailidou & A. Argiriou

  3. Proteomics Research Unit, Center of Basic Research II, Biomedical Research Foundation of the Academy of Athens, 4 Soranou Ephessiou St, 11527, Athens, Greece

    G. Tsangaris

  4. Veterinary Faculty, University of Thessaly, Karditsa, Greece

    G. C. Fthenakis

  5. Department of Animal Production, Technological Educational Institute of Epirus, Arta, Greece

    A. Tzora & I. Skoufos

  6. Cell and Molecular Physiology Unit, Laboratory of Experimental Physiology, Medical School, University of Ioannina, Ioannina, Greece

    S. C. Karkabounas

  7. Scotland’s Rural College and The Roslin Institute University of Edinburgh, Scotland, EH25 9RG, UK

    G. Banos

Authors
  1. S. Michailidou
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. G. Tsangaris
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. G. C. Fthenakis
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. Tzora
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. I. Skoufos
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. S. C. Karkabounas
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. G. Banos
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. A. Argiriou
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. G. Arsenos
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to S. Michailidou.

Ethics declarations

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

Conflict of interest

The authors declare that they have no conflict of interest.

Additional information

Communicated by S. Hohmann.

Electronic supplementary material

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Michailidou, S., Tsangaris, G., Fthenakis, G.C. et al. Genomic diversity and population structure of three autochthonous Greek sheep breeds assessed with genome-wide DNA arrays. Mol Genet Genomics 293, 753–768 (2018). https://doi.org/10.1007/s00438-018-1421-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00438-018-1421-x

Keywords

Search

Navigation