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Suitable in vitro culture of Eimeria bovis meront II stages in bovine colonic epithelial cells and parasite-induced upregulation of CXCL10 and GM-CSF gene transcription

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Abstract

We here established a suitable in vitro cell culture system based on bovine colonic epithelial cells (BCEC) for the development of Eimeria bovis merozoites I and the characterization of early parasite-induced innate epithelial host cell reactions as gene transcription of proinflammatory molecules. Both primary and permanent BCEC (BCECprim and BCECperm) were suitable for E. bovis merozoite I invasion and subsequent development of meronts II leading to the release of viable merozoites II. E. bovis merozoite II failed to develop any further neither into gamont nor oocyst stages in BCEC in vitro. E. bovis merozoite I induced innate epithelial host cell reactions at the level of CXC/CCL chemokines (CXCL1, CXCL8, CXCL10, CCL2), IL-6, and GM-CSF gene transcription. Overall, both BCEC types were activated by merozoite I infections since they showed significantly enhanced gene transcript levels of the immunomodulatory molecules CXCL10 and GM-CSF. However, gene transcription profiles of BCECprim and BCECperm revealed different reaction patterns in response to merozoite I infection with regard to quality and kinetics of chemokine/cytokine gene transcription. Although both BCEC types equally showed most prominent responses for CXCL10 and GM-CSF, the induction of CXCL1, CXCL8, CCL2, and IL-6 gene transcripts varied qualitatively and quantitatively. Our results demonstrate that BCEC seem capable to respond to E. bovis merozoite I infection by the upregulation of CXCL10 and GM-CSF gene transcription and therefore probably contribute to host innate effector mechanisms against E. bovis.

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Acknowledgments

We are deeply indebted to Brigitte Hofmann for her excellent technical assistance in cell culture. This work was supported and financed by the German Research Foundation (DFG, HE 3663/2-1).

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  1. Ivonne Stamm

    Present address: Vet Med Labor GmbH, IDEXX Laboratories, Ludwigsburg, Germany

  2. Christian Menge

    Present address: Friedrich-Loeffler-Institut/Federal Research Institute for Animal Health, Institute of Molecular Pathogenesis, Jena, Germany

Authors and Affiliations

  1. Institute of Parasitology, Biomedical Research Center Seltersberg, Justus Liebig University Giessen, Schubertstr. 81, Giessen, Germany

    Carlos Hermosilla & Anja Taubert

  2. Institute for Hygiene and Infectious Diseases of Animals, Justus Liebig University Giessen, Giessen, Germany

    Ivonne Stamm & Christian Menge

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Hermosilla, C., Stamm, I., Menge, C. et al. Suitable in vitro culture of Eimeria bovis meront II stages in bovine colonic epithelial cells and parasite-induced upregulation of CXCL10 and GM-CSF gene transcription. Parasitol Res 114, 3125–3136 (2015). https://doi.org/10.1007/s00436-015-4531-y

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