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Biting midges monitoring (Diptera: Ceratopogonidae: Culicoides Latreille) in the governate of Monastir (Tunisia): species composition and molecular investigations

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Abstract

The results of entomological studies carried out in the governate of Monastir (Tunisia) in 2009–2010 (captures and emergences from muds) focusing on Culicoides species are presented in the present study. Identification of Culicoides at the species level is based on morphological characters, and a molecular study has been carried out based on mitochondrial DNA cytochrome C oxidase I gene (COI) and D1 and D2 domains of the 28S rDNA. The DNA sequences reported here are related to 10 species (on 25 known) of Culicoides described in Tunisia: Culicoides cataneii-gejgelensis, Culicoides circumscriptus, Culicoides imicola, Culicoides jumineri, Culicoides kingi, Culicoides langeroni, Culicoides newsteadi, Culicoides paolae, Culicoides puncticollis and Culicoides sahariensis. DNA sequencing of the COI gene and D1D2 domains discriminated all morphologically determined species. The choice of D1D2 domains considered as a conserved region is informative for Culicoides species identification. The molecular analyses of COI has grouped both C. circumscriptus, C. puncticollis within two clusters and C. newsteadi within five subclusters. However, C. newsteadi shows relatively deep intraspecific divergence using COI sequences.

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References

  • Ander M, Troell K, Chirico J (2012) Barcoding of biting midges in the genus Culicoides: a tool for species determination. Med Vet Entomol. doi:10.1111/j.1365-2915.2012.01050.x

    Google Scholar 

  • Augot D, Ninio C, Akhoundi M, Lehrter V, Couloux A, Jouet D, Depaquit J (2013) Characterization of two cryptic species: Culicoides stigma and C. parroti (Diptera: Ceratopogonidae) based on barcode regions and morphological description. J Vect Ecol 28:1–6

    Google Scholar 

  • Beckenbach AT, Borkent A (2003) Molecular analysis of the biting midges (Diptera: Ceratopogonidae), based on mitochondrial cytochrome oxidase submit 2. Mol Phylogenet Evol 27:21–35

    Article  CAS  PubMed  Google Scholar 

  • Bonfield JK, Staden R (1996) Experiment files and their application during large-scale sequencing projects. DNA Seq 6:109–117

    CAS  PubMed  Google Scholar 

  • Bonfield JK, Smith KF, Staden R (1995) A new DNA sequence assembly program. Nucleic Acids Res 24:4992–4999

    Article  Google Scholar 

  • Campbell JA, Pelham-Clinton EC (1960) A taxonomic review of the British species Culicoides Latreille (Dipetera: Ceratopogonidae). Proc R Soc Edin B 67:181–302

    Google Scholar 

  • Chaker E, Kremer M (1982) Culicoides of Tunisia: morphological characteristics. Chorology and ecology of species found. Arch Inst Pasteur Tunis 59:511–540

    CAS  PubMed  Google Scholar 

  • Chaker E, Delécolle JC, Kremer M (1980) Variabilité des caractères morphologiques de Culicoides circumscriptus Kieffer 1918. Mise en synonymie de C. kirovabadicus Dzhafarov 1964. Arch Inst Pasteur Tunis 57:33–40

    CAS  PubMed  Google Scholar 

  • Chaker E, Sfari M, Rabhi M, Rouis M, Babba H, Azaiez R (2005) Faunistic note on Culcoides (Diptera, Ceratopogonidae) from the governate of Monastir (Tunisia). Parasite 12:359–361

    Article  CAS  PubMed  Google Scholar 

  • Costa FO, Dewaard JR, Boutillier J, Ratnasingham S, Dooh RT, Hajibabaei M, Hebert PDN (2007) Biological identifications through DNA barcodes: the case of the Crustacea. Can J Fish Aquat Sci 64:272–295

    Article  CAS  Google Scholar 

  • Dallas JF, Cruickshank RH, Linton YM, Nolan DV, Patakakis M, Braverman Y, Capela R, Capela M, Pena I, Meiswinkel R, Ortega MD, Baylis M, Mellor PS, Mordue Luntz AJ (2003) Phylogenetic status and matrilineal structure of the biting midges, Culicoides imicola, in Portugal, Rhodes and Israel. Med Vet Entomol 17:379–387

    Article  CAS  PubMed  Google Scholar 

  • Delécolle JC (1985) Nouvelle contribution à l’étude systématique et iconographique des espèces du genre Culicoides (Diptera: Ceratopogonidae) du Nord-Est de la France. PhD thesis. Université Louis Pasteur de Strasbourg, UER Sciences Vie et Terre

  • Depaquit J, Perrotey S, Lecointre G, Tillier A, Tillier S, Ferté H, Kaltenbach M, Léger N (1998) Systematique moléculaire des Phlebotominae: étude pilote. Paraphylie du genre Phlebotomus. C R Acad Sci III 321:849–855

    Article  CAS  PubMed  Google Scholar 

  • Djerbal M, Délecolle JC (2009) Entomological surveillance of Bluetongue in Algeria. Rev Elev Med Vet Pays Trop 62:81–180

    Google Scholar 

  • Gomulski LM, Meiswinkel R, Delécolle JC, Goffredo M, Gasperi G (2006) Phylogeny of the subgenus Culicoides and related species in Italy, inferred from internal transcribed spacer 2 ribosomal DNA sequences. Med Vet Entomol 20:229–238

    Article  CAS  PubMed  Google Scholar 

  • Hajibabaei M, Janzen DH, Burns JM, Hallwachs W, Hebert PDN (2006) DNA barcodes distinguish specie sof tropical Lepidoptera. Proc Natl Acad Sci U S A 103:968–971

    Article  PubMed Central  PubMed  Google Scholar 

  • Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser 41:95–98

    CAS  Google Scholar 

  • Hurst GD, Jiggins FM (2005) Problems with mitochondrial DNA as a marker in population, phylogeographic and phylogenetic studies: the effects of inherited symbionts. Proc Biol Sci 272:1525–1534

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  • Hwang UW, Kim W, Tautz D, Friedrich M (1998) Molecular phylogenetics at the Felsenstein zone: approaching the Strepsiptera problem using 5.8S and 28S rDNA sequences. Mol Phylogenet Evol 9:470–480

    Article  CAS  PubMed  Google Scholar 

  • Jorgensen RA, Cluster PD (1988) Modes and tempos in the evolution of nuclear ribosomal DNA: new characters for evolutionary studies and new markers for genetic and population studies. Ann Missouri Bot Gard 75:1238–1247

    Article  Google Scholar 

  • Kremer M, Delécolle JC (1974) Variabilité des caractères morphologiques des Culicoides. Ann Parasitol 49:617–619

    CAS  Google Scholar 

  • Kumar S, Tamura K, Nei M (2004) MEGA3: integrated software for Molecular Evolutionary Genetics Analysis and sequence alignment. Oxf J Brief Bioinform 5:150–163

    Article  CAS  Google Scholar 

  • Lassen SB, Nielsen SA, Skovgård H, Kristensen M (2012) Molecular differentiation of Culicoides biting midges (Diptera: Ceratopogonidae) from the subgenus Culicoides Latreille in Denmark. Parasitol Res 110:1765–1771

    Article  CAS  PubMed  Google Scholar 

  • Matsumoto Y, Yanase T, Tsuda T, Noda H (2009) Species-specific mitochondrial gene rearrangements biting midges and vector species identification. Med Vet Entomol 23:47–55

    Article  CAS  PubMed  Google Scholar 

  • Mellor PS, Boorman J, Baylis M (2000) Culicoides biting midges: their role as arbovirus vector. Annu Rev Entomol 45:307–340

    Article  CAS  PubMed  Google Scholar 

  • Miller BR, Crabtree MB, Savage HM (1997) Phylogenetic relationships of the Culicomorpha inferred from 18 s and 5.8 s ribosomal DNA sequences (Diptera: Nematocera). Insect Mol Biol 6:105–114

    Article  CAS  PubMed  Google Scholar 

  • Muñoz-Muñoz F, Talavera S, Pagès N (2011) Geometric morphometrics of the wing in the subgenus Culicoides (Diptera: Ceratopogonidae): from practical implications to evolutionary interpretations. J Med Entomol 48:129–139

    Article  PubMed  Google Scholar 

  • Nielsen SA, Kristensen M (2011) Morphological and molecular identification of species of the Obsoletus group (Diptera: Ceratopogonidae) in Scandinavia. Parasitol Res 109:1133–1141

    Article  PubMed  Google Scholar 

  • Pagès N, Muñoz-Muñoz F, Talavera S, Sarto V, Lorca C, Núñez JI (2009) Identification of cryptic species of Culicoides (Diptera: Ceratopogonidae) in the subgenus Culicoides and development of species-specific PCR assays based on barcode regions. Vet Parasitol 165:298–310

    Article  PubMed  Google Scholar 

  • Perrin A, Cetre-Sossah C, Mathieu B, Baldet T, Delecolle JC, Albina E (2006) Phylogenetic analysis of Culicoides species from France based on nuclear ITS1-rDNA sequences. Med Vet Entomol 20:219–228

    Article  CAS  PubMed  Google Scholar 

  • Raghavendra K, Cornel AJ, Reddy BP, Collins FH, Nanda N, Chandra D, Verma V, Dash AP, Subbarao SK (2009) Multiplex PCR assay and phylogenetic analysis of sequences derived from D2 domain of 28S rDNA distinguished members of the Anopheles culicifacies complex into two groups, A/D and B/C/E. Infect Genet Evol 9:271–277

    Article  CAS  PubMed  Google Scholar 

  • Ramilo D, Garros C, Mathieu B, Benedet C, Allene X, Silva E, Alexandre-Pires G, Da Fonseca IP, Carpenter S, Radrova J, Delecolle JC (2013) Description of Culicoides paradoxalis sp. nov. from France and Portugal (Diptera: Ceratopogonidae). Zootaxa 3753:300

    Article  Google Scholar 

  • Saegerman C, Berkvens D, Mellor PS (2008) Bluetongue epidemiology in the European Union. Emerg Infect Dis 14:539–544

    Article  PubMed Central  PubMed  Google Scholar 

  • Sghaier S, Hammami S, Hammami H, Dkhil A, Delecolle JC (2009) Entomological surveillance of Culicoides (Diptera: Ceratopogonidae), vector of Bluetongue in Tunisia. Rev Elev Med Vet Pays Trop 62:2–4

    Google Scholar 

  • Wenk CE, Kaufmann C, Schaffner F, Mathis A (2012) Molecular characterization of Swiss Ceratopogonidae (Diptera) and evaluation of real-time PCR assays for the identification of biting midges. Vet Parasitol 184:258–266

    Article  CAS  PubMed  Google Scholar 

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Acknowledgments

We thank Sylvette Gobert for proofreading this manuscript.

The authors would like to thank Jean Claude Delécolle for helping with Culicoides determination.

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Authors and Affiliations

  1. Faculty of Pharmacy, Clinical Biology Department, Laboratory of Medical and Molecular Parasitology-Mycology code LR12ES08, Monastir, Tunisia

    D. Slama, E. Chaker & H. Babba

  2. Institute of Parasitology and Tropical Pathology, University of Strasbourg, Strasbourg, France

    B. Mathieu

  3. ANSES, Université de Reims Champagne-Ardenne, SFR Cap santé, EA4688-USC «Transmission vectorielle et épidémiosurveillance de maladies parasitaires» (VECPAR), 51100, Reims, France

    D. Slama, J. Depaquit & D. Augot

  4. Laboratory of Parasitology and Mycology, Tunis, La rabta, Tunisia

    E. Chaker

  5. Laboratory of Maternity and Neonatology Center of Monastir, Monastir, Tunisia

    D. Slama & H. Babba

Authors
  1. D. Slama
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  2. E. Chaker
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  3. B. Mathieu
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  4. H. Babba
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  5. J. Depaquit
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  6. D. Augot
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Correspondence to D. Augot.

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Slama, D., Chaker, E., Mathieu, B. et al. Biting midges monitoring (Diptera: Ceratopogonidae: Culicoides Latreille) in the governate of Monastir (Tunisia): species composition and molecular investigations. Parasitol Res 113, 2435–2443 (2014). https://doi.org/10.1007/s00436-014-3873-1

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  • DOI: https://doi.org/10.1007/s00436-014-3873-1

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