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CircRNA-100284 activates aurora kinase B by inducing methylation of HSP70 via microRNA-217 to promote proliferation of bladder cancer cells

  • Original Article – Cancer Research
  • Published:
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Abstract

Objective

The malignant transformation of normal bladder cells (SV-HUC-1) was induced by arsenite to explore the possible mechanism of circRNA-100284 influencing bladder cancer cell proliferation.

Methods

Normal bladder SV-HUC-1 cells were cultured with 2 μM arsenite to induce malignant transformation. After 0, 3, 6, 12, and 24 h of culture, the expression level of circRNA-100284 in cells was detected by quantitative real-time PCR. Western blotting assays were used to detect the expression levels of EZH2 and cyclin-D1 proteins in cells treated with different media. Cell cycle was analyzed by flow cytometry. In addition, through cell transfection and CCK-8 experiments, the effect and mechanism of circRNA-100284 targeting microRNA-217 on proliferation was determined. The interaction between HSP70 methylation and Aurora-B was determined by Western blotting and immunoprecipitation experiments.

Results

With prolonged contact time with arsenite, the expression level of circRNA-100284 in cells increased continuously (P < 0.05). Western blotting assays showed that the expression levels of EZH2 and cyclin-D1 proteins in arsenite-transformed cells increased. Flow cytometry and CCK-8 showed that circRNA-100284 accelerated cell cycle transition and cell proliferation through miR-217. Finally, after culturing human bladder cancer T24 cells, combined with immunoprecipitation and in vitro kinase experiments, it was found that K561- dimethyl HSP70 activated Aurora-B, thus promoting the proliferation of bladder cancer cells.

Conclusion

CircRNA-100284 activates aurora kinase B by inducing methylation of HSP70 via microRNA-217 to promote the proliferation of bladder cancer cells.

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Data availability

The datasets generated and analyzed during the current study are available from the corresponding author on reasonable request.

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Funding

No funding was received for this study.

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Authors and Affiliations

  1. Department of Urology, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, No.1 Shuaifuyuan Wangfujing, Dongcheng, Beijing, 100730, China

    Zhong-Ming Huang, Hai Wang & Zhi-Gang Ji

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  1. Zhong-Ming Huang
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  2. Hai Wang
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  3. Zhi-Gang Ji
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Contributions

ZG J and ZMH contributed to the conception and design of the study; ZMH and HW contributed to the acquisition of data and performed the experiments; ZMH contributed to the analysis of data; ZMH wrote the manuscript; all authors reviewed and approved the final version of the manuscript.

Corresponding author

Correspondence to Zhi-Gang Ji.

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Conflict of interests

The authors declare that they have no competing interests.

Ethical approval

This research was approved by the Ethics Committee of Peking Union Medical College Hospital. All the procedures were performed in accordance with the 1964 Helsinki Declaration and its later amendments.

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Huang, ZM., Wang, H. & Ji, ZG. CircRNA-100284 activates aurora kinase B by inducing methylation of HSP70 via microRNA-217 to promote proliferation of bladder cancer cells. J Cancer Res Clin Oncol 147, 703–712 (2021). https://doi.org/10.1007/s00432-020-03468-4

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  • DOI: https://doi.org/10.1007/s00432-020-03468-4

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