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Prevalence and significance of M541L single nucleotide polymorphism in the central European cohort of gastrointestinal stromal tumor patients

  • Original Article – Clinical Oncology
  • Published:
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Abstract

Background

Single nucleotide polymorphisms can create a genetic microenvironment in some tumors that affects the course of treatment, resistance, etc. Whether single nucleotide polymorphisms have an impact on gastrointestinal stromal tumor (GIST) development and disease progression is not yet accurately verified. KIT SNPM541L in exon 10 correlates with a worse prognosis of many cancers. The impact of KIT SNPM541L in GISTs is relatively unknown and, therefore, its analyses could have potential in patient therapy and could provide more detailed information on tumor character, clinical presentation, or tumor behavior in treatment.

Aim

The aim of the study was the analysis of the biological and clinical significance of the KIT SNPM541L polymorphism in exon 10.

Materials and methods

Paraffin sample tissues were obtained from the National GIST Register in Martin. Retrospective samples from 177 GIST patients were divided into several groups. Detection of SNPM541L was performed by Sanger sequencing. Statisitical analyses were performed to determine the prevalence of KIT SNPM541L in the Slovak GIST cohort, to search for correlation between c-KIT status and clinicopathological, molecular and biological data.

Results

Overall, 29 samples out of 177 showed KIT SNPM541L polymorphism.

Conclusion

Our results do not support the association between KIT SNPM541L and increased risk of relapse in localized primary GISTs. Additionally, we found a positive correlation between KIT SNPM541L occurrence and earlier onset of relapse in PDGFRa and WT subgroup of GISTs.

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Funding

This publication is the result of the project implementation: “CENTER OF EXCELLENCE FOR RESEARCH IN PERSONALIZED THERAPY (CEVYPET)”, ITMS: 26220120053 supported by the Operational Programme Research and Innovation funded by the ERDF. This study was also supported by the project of Slovak Research and Development Agency no. APVV-16-0066 as well as by the Grant of The Ministry of Education, Science, Research and Sport of the Slovak Republic no.VEGA-1/0380/18.

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Authors and Affiliations

  1. Biomedical Center Martin, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovakia

    Karin Jasek, Marian Grendar, Bibiana Malicherova, Ivana Kasubova, Lukas Plank & Zora Lasabova

  2. Department of Pathological Anatomy, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovakia

    Andrea Stanclova, Peter Szepe & Lukas Plank

  3. Department of Molecular Biology and Genomics, Jessenius Faculty of Medicine in Martin, Comenius University in Bratislava, Martin, Slovakia

    Tatiana Burjanivova & Zora Lasabova

  4. Department of Medicine, A.O.U.I. Policlinico G.B. Rossi and University of Verona, Verona, Italy

    Rachele Ciccocioppo

  5. Faculty of Medicine, University of Oviedo, Central University Hospital of Asturias (HUCA), Oviedo, Spain

    Luis Rodrigo

  6. 2nd Department of Internal Medicine, Faculty of Medicine, Masaryk University and St. Anne’S University Hospital, Brno, Czech Republic

    Robert Prosecky

  7. Research and Development Services, Brno, Czech Republic

    Peter Kruzliak

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  1. Karin Jasek
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  8. Rachele Ciccocioppo
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  11. Peter Kruzliak
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Correspondence to Peter Kruzliak or Zora Lasabova.

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Jasek, K., Grendar, M., Stanclova, A. et al. Prevalence and significance of M541L single nucleotide polymorphism in the central European cohort of gastrointestinal stromal tumor patients. J Cancer Res Clin Oncol 147, 1203–1215 (2021). https://doi.org/10.1007/s00432-020-03410-8

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