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Detection of disseminated tumor cells in bone marrow and circulating tumor cells in blood of patients with early-stage male breast cancer

  • Original Article – Cancer Research
  • Published:
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Abstract

Background

Male breast cancer (MBC) is a rare malignant disease, accounting for <1 % of all breast cancers (BCs). Treatment of men with early-stage BC is based on standards established in female BC. Prognostic or predictive markers to guide therapeutic decisions, in particular in early-stage male BC, are missing. Here, we explored whether disseminated tumor cells (DTC) in bone marrow (BM) and circulating tumor cells (CTC) in blood could be suitable biomarkers in male BC.

Patients and methods

Five male patients (pT2–4, pN0–2, M0) with hormone receptor-positive, HER2-negative, and ductal primary BC (median age 70 years, range 51–73) were enrolled in a prospective study of patients with early-stage breast cancer. Here, we analyze the male subgroup. DTC in BM were analyzed before therapy and identified by immunocytochemistry using the pancytokeratin antibody A45B/B3. Blood samples (10 ml) were analyzed for CTC using the AdnaTest BreastCancer (AdnaGen AG, Langenhagen, Germany).

Results

DTC were found in three out of five male patients (60 %) with two DTC detected in one patient and one DTC detected in each of the other two patients. This is compared to a detection rate of 25–40 % in pooled analyses of female patients. CTC were only found in one of five patients. After a median follow-up time of 3 years (range 1–10 years), all patients were still alive and free of relapse.

Conclusion

The prevalence of DTC and CTC in male BC seems comparable with female BC. No prognostic relevance could be documented in this small population. A prospective study or at least larger cases series will be required to assess the prognostic or predictive value of DTC and CTC in this rare disease.

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Abbreviations

CTC:

Circulating tumor cells

DTC:

Disseminating tumor cells

ER:

Estrogen receptor

PR:

Progesterone receptor

HR:

Hormone receptor

BM:

Bone marrow

Pt:

Patient

MBC:

Male breast cancer

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Conflict of interest

The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Department of Medical Oncology, West German Cancer Center, University Hospital Essen, University of Duisburg-Essen, Hufelandstrasse 55, 45122, Essen, Germany

    Mitra Tewes, Anja Welt & Martin Schuler

  2. Department of Gynecology and Obstetrics, West German Cancer Center, University Hospital Essen, University of Duisburg-Essen, 45122, Essen, Germany

    Sabine Kasimir-Bauer, Rainer Kimmig & Bahriye Aktas

  3. German Cancer Consortium (DKTK), 69129, Heidelberg, Germany

    Martin Schuler

Authors
  1. Mitra Tewes
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  2. Sabine Kasimir-Bauer
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  3. Anja Welt
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  4. Martin Schuler
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  5. Rainer Kimmig
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  6. Bahriye Aktas
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Correspondence to Mitra Tewes.

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Tewes, M., Kasimir-Bauer, S., Welt, A. et al. Detection of disseminated tumor cells in bone marrow and circulating tumor cells in blood of patients with early-stage male breast cancer. J Cancer Res Clin Oncol 141, 87–92 (2015). https://doi.org/10.1007/s00432-014-1784-2

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  • DOI: https://doi.org/10.1007/s00432-014-1784-2

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