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SIRT1 gene variants are related to risk of childhood obesity

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Abstract

Obesity is a multifactorial disorder resulting from the interaction between genetic, psychological, physical, environmental, and socioeconomic factors. SIRT1 gene has important effects on the regulation of adiponectin, caloric restriction, insulin sensitivity, coronary atherosclerosis, and cardiovascular diseases. The aim of this study was to investigate the association between childhood obesity and SIRT1 gene polymorphisms regarding rs7895833 A > G in the promoter region, rs7069102 C > G in intron 4, and rs2273773 C > T in exon 5 using PCR-CTPP method in 120 obese and 120 normal weight children. In this study, BMI, systolic and diastolic blood pressure, LDL cholesterol, triglyceride, and insulin levels were significantly higher and HDL-cholesterol levels were significantly lower in obese children compared to normal weight children. For rs7895833 A > G, the rate of having AG genotype and G allele was significantly higher in obese children compared to non-obese group (p < 0.001). The risk for obesity was increased by 1.9 times in G allele carriers; therefore, A allele may be protective against obesity. Both study groups had CT heterozygote genotype for rs2273773 C > T. There was no significant difference for rs7069102 C > G gene polymorphism between groups.

Conclusion: This is the first study reporting an association between SIRT1 gene polymorphisms and obesity in children.

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Abbreviations

BMI:

Body mass index

CRTC2 or TORC2:

CREB-regulated transcription coactivator 2

ERF:

Erasmus Rucphen Family

FXR:

Farnesoid X receptor

HDL:

High-density lipoprotein

LDL:

Low-density lipoprotein

LXR:

Liver X receptor

PCR-CTPP:

Polymerase chain reaction with confronting two-pair primers

PGC-1α:

Peroxisome proliferator-activated receptor gamma coactivator 1-alpha

PPARγ:

Proliferator-activated receptor gamma

SIRT1:

Silent mating type information regulation 2 homolog 1

SNP:

Single-nucleotide polymorphism

SREBP:

Sterol regulatory element-binding protein

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Acknowledgments

This study was supported by grants from the University Research Council, Bezmialem Vakif University (BAP: 9.2012/15). The authors would like to thank Assoc. Prof. Dr. Arzu Ergen for her help in this study.

Conflict of interest

The authors declare that there are no conflicts of interest.

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Authors and Affiliations

  1. Department of Medical Biology, Faculty of Medicine, Bezmialem Vakif University, Adnan Menderes Bulvarı, Vatan Caddesi, 34093, Fatih-Istanbul, Turkey

    Ulkan Kilic, Ozlem Gok & Birsen Elibol-Can

  2. Department of Medical Biology, Faculty of Medicine, Istanbul Medipol University, 34083, Istanbul, Turkey

    Ulkan Kilic

  3. Department of Pediatric Endocrinology and Metabolism, Faculty of Medicine, Bezmialem Vakif University, 34093, Istanbul, Turkey

    Ilker Tolga Ozgen

  4. Department of Pediatrics, Faculty of Medicine, Bezmialem Vakif University, 34093, Istanbul, Turkey

    Ufuk Erenberk & Mehmet Rusen Dundaroz

  5. Department of Biostatistics, Faculty of Medicine, Bezmialem Vakif University, 34093, Istanbul, Turkey

    Omer Uysal

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  1. Ulkan Kilic
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Correspondence to Ulkan Kilic.

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Communicated by Beat Steinmann

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Kilic, U., Gok, O., Elibol-Can, B. et al. SIRT1 gene variants are related to risk of childhood obesity. Eur J Pediatr 174, 473–479 (2015). https://doi.org/10.1007/s00431-014-2424-1

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  • DOI: https://doi.org/10.1007/s00431-014-2424-1

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