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Seed priming with NaCl helps to improve tissue tolerance, potassium retention ability of plants, and protects the photosynthetic ability in two different legumes, chickpea and lentil, under salt stress

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Abstract

Main conclusion

Seed priming with NaCl mimicked the conditions of natural priming to improve the tissue tolerance nature of sensitive legumes, which helps to maintain survivability and yield in mildly saline areas.

Abstract

Seed priming with NaCl is a seed invigoration technique that helps to improve plant growth by altering Na+ and K+ content under salt stress. Legumes are overall sensitive to salt and salinity hampers their growth and yield. Therefore, a priming (50 mM NaCl) experiment was performed with two different legume members [Cicer arietinum cv. Anuradha and Lens culinaris cv. Ranjan] and different morpho-physiological, biochemical responses at 50 mM, 100 mM, and 150 mM NaCl and molecular responses at 150 mM NaCl were studied in hydroponically grown nonprimed and primed members. Similarly, a pot experiment was performed at 80 mM Na+, to check the yield. Tissue Na+ and K+ content suggested NaCl-priming did not significantly alter the accumulation of Na+ among nonprimed and primed members but retained more K+ in cells, thus maintaining a lower cellular Na+/K+ ratio. Low osmolyte content (e.g., proline) in primed members suggested priming could minimize their overall osmolytic requirement. Altogether, these implied tissue tolerance (TT) nature might have improved in case of NaCl-priming as was also reflected by a better TT score (LC50 value). An improved TT nature enabled the primed plants to maintain a significantly higher photosynthetic rate through better stomatal conductance. Along with this, a higher level of chlorophyll content and competent functioning of the photosynthetic subunits improved photosynthetic performance that ensured yield under stress. Overall, this study explores the potential of NaCl-priming and creates possibilities for considerably sensitive members; those in their nonprimed forms have no prospect in mildly saline agriculture.

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Abbreviations

AtpF:

Plastid protein required for assembly of the ATP synthase complex

PsaB:

Core subunit of PS I

PsbN:

Plastid-encoded PS II assembly factor

RWC:

Relative water content

Y(NO):

Quantum yield of non-regulated energy dissipation

References

  • Afzal I, Rauf S, Basra SMA, Murtaza G (2008) Halopriming improves vigor, metabolism of reserves and ionic contents in wheat seedlings under salt stress. Plant Soil Environ 54:382–388

    Article  CAS  Google Scholar 

  • Afzal I, Butt A, Rehman HU, Basra SMA, Afzal A (2012) Alleviation of salt stress in fine aromatic rice by seed priming. Aust J Crop Sci 6(10):1401–1407

    CAS  Google Scholar 

  • Allakhverdiev SI, Feyziev YM, Ahmed A, Hayashi H, Aliev JA, Klimov VV et al (1996) Stabilization of oxygen evolution and primary electron transport reactions in photosystem II against heat stress with glycinebetaine and sucrose. J Photochem Photobiol B Biol 34(2–3):149–157

    Article  CAS  Google Scholar 

  • Anderson MC (1967) Photon flux, chlorophyll content, and photosynthesis under natural conditions. Ecology 48(6):1050–1053

    Article  Google Scholar 

  • Arif Y, Singh P, Siddiqui H, Bajguz A, Hayat S (2020) Salinity induced physiological and biochemical changes in plants: an omic approach towards salt stress tolerance. Plant Physiol Biochem 156:64–77

    Article  CAS  PubMed  Google Scholar 

  • Arnon DI (1949) Copper enzymes in isolated chloroplasts polyphenoloxidase in Beta vulgaris. Plant Physiol 24:1–15

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Ashraf M, Waheed A (1993) Responses of some local/exotic accessions of lentils (Lens culinaris Medic.) to salt stress. J Agric Crop Sci 170(2):103–112

    Article  CAS  Google Scholar 

  • Bakht J, Shafi M, Shah SR (2011) Response of maize cultivars to various priming sources. Pak J Bot 43:205–212

    CAS  Google Scholar 

  • Barrs HD, Weatherly PE (1962) A re-examination of the relative turgidity technique for estimating water deficits in leaves. J Biol Sci 15(3):413–428

    Google Scholar 

  • Bates LD, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207

    Article  CAS  Google Scholar 

  • Bauer H, Senser M (1979) Photosynthesis of ivy leaves (Hedera helix L.) after heat stress. II. Activity of ribulose bisphosphate carboxylase, Hill reaction and chloroplast ultrastructure. Z Plant Physiol 91:359–369

    CAS  Google Scholar 

  • Biswas S, Paul A, Biswas AK (2020) Potential of seed halopriming in mitigating NaCl induced adversities on nitrogen metabolism in legume crops. Leg Res 45:73–81

    Google Scholar 

  • Blumwald E (1987) Tonoplast vesicles as a tool in the study of ion transport at the plant vacuole. Physiol Plant 69:731–734

    Article  CAS  Google Scholar 

  • Cayuela E, Perez-Alfocea F, Caro M, Bolarin MC (1996) Priming of seeds with NaCl induces physiological changes in tomato plants grown under salt stress. Physiol Plant 96:231–236

    Article  CAS  Google Scholar 

  • Chakraborty K, Mondal S, Ray S, Pankajini S, Pradhan B, Chattopadhyay K, Meera KK, Swain P (2020) Tissue tolerance coupled with ionic discrimination can potentially minimize the energy cost of salinity tolerance in rice. Front Plant Sci 11:265

    Article  PubMed  PubMed Central  Google Scholar 

  • Daloso MD, Medeiros BD, Anjos L, Yoshida T, Araujo LW, Fernie RA (2017) Metabolism within the specialized guard cells of plants. New Phytol 216:1018–1033

    Article  CAS  PubMed  Google Scholar 

  • Flowers TJ, Gaur PM, Gowda CLL, Krishnamurthy L, Samineni S, Siddique KHM, Turner NC, Vadez V, Varshney RK, Colmer TD (2010) Salt sensitivity in chickpea. Plant Cell Environ 33(4):490–509

    Article  CAS  PubMed  Google Scholar 

  • Foyer C, Lam HM, Nguyen H, Siddique KHM, Varshney RK et al (2016) Neglecting legumes has compromised human health and sustainable food production. Nat Plants 2:16112

    Article  PubMed  Google Scholar 

  • Fukuda A, Nakamura A, Hara N, Toki S, Tanaka Y (2011) Molecular and functional analyses of rice NHX-type Na+/H+ antiporter genes. Planta 233:175–188

    Article  CAS  PubMed  Google Scholar 

  • Ghassemi-Golezani K, Asadi-Danalo A, Shafagh-Kalvanagh J (2012) Seed vigour and field performance of lentil (Lens culinaris Medik.) under different irrigation treatments. Res Crops 13(1):113–117

    Google Scholar 

  • Ghoulam D, Foursy A, Fares K (2002) Effects of salt stress on growth, inorganic ions and proline accumulation in relation to osmotic adjustment in five beet cultivars. Environ Exp Bot 47:39–50

    Article  CAS  Google Scholar 

  • Grieve CM, Grattan SR (1983) Rapid assay for determination of water soluble quaternary ammonium compounds. Plant Soil 70:303–307

    Article  CAS  Google Scholar 

  • Gupta B, Huang B (2014) Mechanism of salinity tolerance in plants: physiological, biochemical, and molecular characterization. Int J Genomics. https://doi.org/10.1155/2014/701596

    Article  PubMed  PubMed Central  Google Scholar 

  • Hanin M, Ebel C, Ngom M, Laplaze L, Masmoudi K (2016) New insights on plant salt tolerance mechanisms and their potential use for breeding. Front Plant Sci 7:1787

    Article  PubMed  PubMed Central  Google Scholar 

  • Hill R, Bendall FAY (1960) Function of the two cytochrome components in chloroplasts: a working hypothesis. Nature 186(4719):136–137

    Article  CAS  Google Scholar 

  • Iqbal M, Ashraf M (2007) Seed preconditioning modulates growth, ionic relations and photosynthetic capacity in adult plants of hexaploid wheat under salt stress. J Plant Nutr 30:381–396

    Article  CAS  Google Scholar 

  • Jisha KC, Puthur JT (2014) Halopriming of seeds imparts tolerance to NaCl and PEG induced stress in Vigna radiata (L.) Wilczek varieties. Physiol Mol Biol Plants 20:303–312

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Jusovik M, Velitchkova MY, Misheva SP (2018) Photosynthetic responses of a wheat mutant (Rht-B1C) with altered DELLA proteins to salt stress. J Plant Growth Regul 37:645–656

    Article  Google Scholar 

  • Khan HA, Siddique KH, Munir R, Colmer TD (2015) Salt sensitivity in chickpea: growth, photosynthesis, seed yield components and tissue ion regulation in contrasting genotypes. J Plant Physiol 182:1–12

    Article  CAS  PubMed  Google Scholar 

  • Kiani-Pouya A, Rasouli F, Rabbi B, Falakboland Z, Yong M, Chen ZH et al (2020) Stomatal traits as a determinant of superior salinity tolerance in wild barley. J Plant Physiol 245:153108

    Article  CAS  PubMed  Google Scholar 

  • Kotula L, Khan HA, Quealy J, Turner NC, Siddique KHM, Clode PL, Colmer TD (2015) Salt sensitivity in chickpea (Cicer arietinum L.): ions in reproductive tissues and yield components in contrasting genotypes. Plant Cell Environ 38(8):1565–1577

    Article  CAS  PubMed  Google Scholar 

  • Kotula L, Clode PL, Jimenez JDLC, Colmer TD (2019) Salinity tolerance in chickpea is associated with the ability to ‘exclude’ Na from leaf mesophyll cells. J Exp Bot 70:4991–5002

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Kumar P, Sharma PK (2020) Soil salinity and food security in India. Front Sustain Food Syst 4:533781

    Article  Google Scholar 

  • Laemmli KU (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Article  CAS  PubMed  Google Scholar 

  • Liu X, Quan W, Bartels D (2022) Stress memory responses and seed priming correlate with drought tolerance in plants: an overview. Planta 255(2):45

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods 25:402–408

    Article  CAS  PubMed  Google Scholar 

  • Long SP, Zhu X-G, Naidu SL, Ort DR (2006) Can improvement in photosynthesis increase crop yields? Plant Cell Environ 29(3):315–330

    Article  CAS  PubMed  Google Scholar 

  • Lowry OH, Rosbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275

    Article  CAS  PubMed  Google Scholar 

  • Mann A, Kumar A, Sanwal SK, Sharma PC (2020) Sustainable production of pulses under saline lands in India. In: Hasanuzzaman M (ed) Legume crops—prospects, production and uses. IntechOpen, London

    Google Scholar 

  • Mishra P, Yonar A, Yonar H, Kumari B, Abotaleb M, Das SS, Patil SG (2021) State of the art in total pulse production in major states of India using ARIMA techniques. Curr Res Nutr Food Sci 4:800–806

    Google Scholar 

  • Munns R, Tester M (2008) Mechanisms of salinity tolerance. Annu Rev Plant Biol 59:651–681

    Article  CAS  PubMed  Google Scholar 

  • Munns R, James RA, Gilliham M, Flowers TJ, Colmer TD (2016) Tissue tolerance: an essential but elusive trait for salt-tolerant crops. Funct Plant Biol 43(12):1103–1113

    Article  CAS  PubMed  Google Scholar 

  • Murata N, Takahashi S, Nishiyama Y, Allakhverdiev SI (2007) Photoinhibition of photosystem II under environmental stress. Biochim Biophys Acta Bioenergy 1767(6):414–421

    Article  CAS  Google Scholar 

  • Patterson JH, Newbigin ED, Tester M, Bacic A, Roessner U (2009) Metabolic responses to salt stress of barley (Hordeum vulgare L.) cultivars, Sahara and Clipper, which differ in salinity tolerance. J Exp Bot 60:4089–4103

    Article  PubMed  PubMed Central  Google Scholar 

  • Paul A, Biswas S, Banerjee R, Mukherjee A, Biswas AK (2021) Halopriming imparts salt tolerance by reducing oxidative, osmotic stress and DNA damage in five different legume varieties. Leg Res. https://doi.org/10.18805/LR-4723

    Article  Google Scholar 

  • Pei Z-M, Murata Y, Benning G, Thomine S, Klüsener B, Allen GJ, Grill E, Schroeder JI (2000) Calcium channels activated by hydrogen peroxide mediate abscisic acid signalling in guard cells. Nature 406:731–734

    Article  CAS  PubMed  Google Scholar 

  • Percey WJ, McMinn A, Bose J et al (2016) Salinity effects on chloroplast PS II performance in glycophytes and halophytes. Funct Plant Biol 43:1003–1015

    Article  CAS  PubMed  Google Scholar 

  • Porcel R, Redondo-Gómez S, Mateos-Naranjo E, Aroca R, Garcia R, Ruiz-Lozano JM (2015) Arbuscular mycorrhizal symbiosis ameliorates the optimum quantum yield of photosystem II and reduces non-photochemical quenching in rice plants subjected to salt stress. J Plant Physiol 185:75–83

    Article  CAS  PubMed  Google Scholar 

  • Pradhan B, Chakraborty K, Prusty N, Shakyawar D, Mukherjee AK, Chattopadhyay K et al (2018) Distinction and characterization of rice genotypes tolerant to combined stresses of salinity and partial submergence, proved by high resolution chlorophyll fluorescence imaging system. Funct Plant Biol 46:248–261

    Article  Google Scholar 

  • Prusty MR, Kim SR, Vinarao R, Entila F, Egdare J, Diaz MGQ et al (2018) Newly identified wild rice accessions conferring high salt tolerance might use a tissue tolerance mechanism in leaf. Front Plant Sci 9:417

    Article  PubMed  PubMed Central  Google Scholar 

  • Pushpavalli R, Quealy J, Colmer TD, Turner NC, Siddique KHM, Rao MV, Vadez V (2015) Salt stress delayed flowering and reduced reproductive success of chickpea (Cicer arietinum), a response associated with Na+ accumulation in leaves. J Agron Crop Sci 197:214–227

    Google Scholar 

  • Raven JA (1985) Regulation of pH and generation of osmolarity in vascular plants: a cost-benefit analysis in relation to efficiency of use of energy, nitrogen and water. New Phytol 101:25–77

    Article  CAS  PubMed  Google Scholar 

  • Rehman HU, Nawaz Q, Basra SMA, Afzal I, Yasmeen A, Hassan F (2014) Seed priming influence on early crop growth, phonological development and yield performance of linola (Linum usitatissimum L.). J Intgr Agric 13(5):990–996

    Article  Google Scholar 

  • Rodrigues O, Shan L (2021) Stomata in a state of emergency: H2O2 is the target locked. Trends Plant Sci 27(3):274–286. https://doi.org/10.1016/j.tplants.2021.10.002

    Article  CAS  PubMed  Google Scholar 

  • Sanderson LA, Caron CT, Tan R, Shen Y, Liu R, Bett KE (2019) KnowPulse: a web-resource focussed on diversity data for pulse crop improvement. Front Plant Sci 10:965

    Article  PubMed  PubMed Central  Google Scholar 

  • Savvides A, Shawkat A, Mark T, Fotopoulos V (2016) Chemical priming of plants against multiple abiotic stresses: Mission possible? Trends Plant Sci 21:329–340

    Article  CAS  PubMed  Google Scholar 

  • Schägger H (2006) Tricine-SDS-PAGE. Nat Protoc 1:16–21

    Article  PubMed  Google Scholar 

  • Schroeder J (2003) Knockout of the guard cell K+ out channel and stomatal movements. Proc Natl Acad Sci USA 100:4976–4977

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Shabala S, Pottosin II (2010) Potassium and potassium-permeable channels in plant salt tolerance. In: Demidchik V, Maathuis F (eds) Ion channels and plant stress responses. Signaling and communications in plants. Springer, Berlin, pp 87–110. https://doi.org/10.1007/978-3-642-10494-7_5

    Chapter  Google Scholar 

  • Shavrukov Y, Langridge P, Tester M (2009) Salinity tolerance and sodium exclusion in genus Triticum. Breed Sci 59:671–678

    Article  CAS  Google Scholar 

  • Simkin AJ, López-Calcagno PE, Raines CA (2019) Feeding the world: improving photosynthetic efficiency for sustainable crop production. J Exp Bot 70(4):1119–1140

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Singh J, Singh V, Sharma PC (2018) Elucidating the role osmotic, ionic and major salt responsive transcript components towards salinity tolerance in contrasting chickpea (Cicer arietinum L.) genotypes. Physiol Mol Biol Plant 24:441–453

    Article  CAS  Google Scholar 

  • Sinha P, Saxena RK, Singh VK, Krishnamurthy L, Varshney RK (2015) Selection and validation of housekeeping genes as reference for gene expression studies in pigeonpea (Cajanus cajan) under heat and salt stress conditions. Front Plant Sci 6:1071

    Article  PubMed  PubMed Central  Google Scholar 

  • Solis CA, Yong MT, Vinarao R, Jena K, Holford P, Shabala L, Zhou M, Shabala S, Chen Z-H (2020) Back to the wild: on a quest for donors toward salinity tolerant rice. Front Plant Sci 11:323

    Article  PubMed  PubMed Central  Google Scholar 

  • Sudhir P, Murthy S (2004) Effects of salt stress on basic processes of photosynthesis. Photosynthetica 42:481–486

    Article  CAS  Google Scholar 

  • Sun Y, Kong X, Li C, Liu Y, Ding Z (2015) Potassium retention under salt stress is associated with natural variation in salinity tolerance among Arabidopsis accessions. PLoS ONE 10:e0124032

    Article  PubMed  PubMed Central  Google Scholar 

  • Theerakulpisut P, Kanawapee N, Panwong B (2016) Seed priming alleviated salt stress effects on rice seedlings by improving Na+/K+ and maintaining membrane integrity. Int J Plant Biol 7:6402

    Article  Google Scholar 

  • Torabi S, Umate P, Manavski N, Plöchinger M, Kleinknecht L, Bogireddi H, Herrmann GR, Wanner G, Schröder PW, Meure J (2014) PsbN is required for the assembly of the photosystem II reaction center in Nicotiana tabacum. Plant Cell 26:1183–1199

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Turner NC, Colmer TD, Quealy J, Pushpavalli R, Krishnamurthy L, Kaur J, Singh G, Siddique KHM, Vadez V (2013) Salinity tolerance and ion accumulation in chichkpea (Cicer arietinum L.) subjected to salt stress. Plant Soil 365:347–361

    Article  CAS  Google Scholar 

  • Tyerman SD, Munns R, Fricke W, Arsova B, Barkla BJ, Bose J et al (2019) Energy costs of salinity tolerance in crop plants. New Phytol 221:25–29

    Article  PubMed  Google Scholar 

  • Untergasser A, Cutcutache I, Koressaar T, Ye J, Faircloth CB, Remm M, Rozen GS (2012) Primer 3 - new capabilities and interfaces. Nucleic Acids Res 40:e115–e115

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Wungrampha S, Joshi R, Singla-pareek SL, Pareek A (2018) Photosynthesis and salinity: are these mutually exclusive? Photosynthetica 56:366–381

    Article  CAS  Google Scholar 

  • Yamori W, Shikanai T (2016) Physiological functions of cyclic electron transport around photosystem I in sustaining photosynthesis and plant growth. Annu Rev Plant Biol 67:81–106

    Article  CAS  PubMed  Google Scholar 

  • Yu Y, Assmann SM (2016) The effect of NaCl on stomatal opening in Arabidopsis wild type and agb1 heterotrimeric G-protein mutant. Plant Signal Behav 11:e1085275

    Article  PubMed  Google Scholar 

  • Zahra N, Hinai MSA, Hafeez MB, Rehman A, Wahid A, Siddique KHM, Farooq M (2022) Regulation of photosynthesis under salt stress and associated salt tolerance mechanisms. Plant Physiol Biochem 178:55–69

    Article  CAS  PubMed  Google Scholar 

  • Zeleny L (1961) Ways to test seeds for moisture. In: Stefferud A (ed) Seeds. The Yearbook of Agriculture. USDA, Washington, pp 443–447

    Google Scholar 

  • Zhang WD, Wang P, Bao Z, Ma Q, Duan LJ, Bao AK et al (2017) SOS1, HKT1;5, and NHX1 synergistically modulate Na+ homeostasis in the halophytic grass Puccinellia tenuiflora. Front Plant Sci 8:576

    PubMed  PubMed Central  Google Scholar 

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Acknowledgements

The work was financially supported by The Department of Science and Technology, Government of West Bengal under Sanction No. 1012 (Sanc.)/ST/P/S&T/2G-2/2013. We revere gratitude towards DST-FIST and UGC-CAS for the instrumental facilities in Department of Botany, University of Calcutta. The DBT-IPLS facility, UGC-UPE facility, in the University of Calcutta is recognized for the confocal microscope. AP would like to thank University of Calcutta for her Ph.D. registration and ICAR-Central Soil Salinity Research Institute, Regional Research Station Canning Town for their guidance while conducting experiments under soil conditions. SM would like to thank University Grant Commission (UGC) for the NET-SRF fellowship and Utkal University for his Ph.D. registration.

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  1. Alivia Paul and Subhankar Mondal contributed equally to this work.

Authors and Affiliations

  1. Plant Physiology and Biochemistry Laboratory, CAS, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700019, India

    Alivia Paul & Asok K. Biswas

  2. Cell Biology Laboratory, CAS, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, Kolkata, 700019, India

    Alivia Paul, Asmita Pal & Rita Kundu

  3. Crop Physiology and Biochemistry Division, ICAR-National Rice Research Institute, Cuttack, Odisha, 753006, India

    Subhankar Mondal & Koushik Chakraborty

  4. Department of Botany, Utkal University, Vani Vihar, Bhubaneswar, Odisha, 751004, India

    Subhankar Mondal

  5. Regional-Cum-Facilitation Centre (Eastern Region), National Medical Plants Board (NMPB), Ministry of Ayush, Govt. of India, Jadavpur University, Kolkata, 700032, India

    Soumyajit Biswas & Asis Mazumder

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  1. Alivia Paul
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Paul, A., Mondal, S., Pal, A. et al. Seed priming with NaCl helps to improve tissue tolerance, potassium retention ability of plants, and protects the photosynthetic ability in two different legumes, chickpea and lentil, under salt stress. Planta 257, 111 (2023). https://doi.org/10.1007/s00425-023-04150-y

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