Abstract
Main conclusion
We identified a typical rice premature senescence leaf mutant 86 (psl86) and exhibited the first global ubiquitination data during rice leaf senescence.
Abstract
Premature leaf senescence affects the yield and quality of rice, causing irreparable agricultural economic losses. In this study, we reported a rice premature senescence leaf mutant 86 (psl86) in the population lines of rice (Oryza sativa) japonica cultivar ‘Yunyin’ (YY) mutagenized using ethyl methane sulfonate (EMS) treatment. Immunoblotting analysis revealed that a higher ubiquitination level in the psl86 mutant compared with YY. Thus, we performed the proteome and ubiquitylome analyses to identify the differential abundance proteins and ubiquitinated proteins (sites) related to leaf senescence. Among 885 quantified lysine ubiquitination (Kub) sites in 492 proteins, 116 sites in 94 proteins were classified as up-regulated targets and seven sites in six proteins were classified as down-regulated targets at a threshold of 1.5. Proteins with up-regulated Kub sites were mainly enriched in the carbon fixation in photosynthetic organisms, glycolysis/gluconeogenesis and the pentose phosphate pathway. Notably, 14 up-regulated Kub sites in 11 proteins were enriched in the carbon fixation in photosynthetic organism pathway, and seven proteins (rbcL, PGK, GAPA, FBA5, ALDP, CFBP1 and GGAT) were down-regulated, indicating this pathway is tightly regulated by ubiquitination during leaf senescence. To our knowledge, we present the first global data on ubiquitination during rice leaf senescence.
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Abbreviations
- CBC:
-
Calvin–Benson cycle
- EMS:
-
Ethyl methane sulfonate
- GO:
-
Gene ontology
- KEGG:
-
Kyoto encyclopedia of genes and genomes
- Kub:
-
Lysine ubiquitination
- psl86 :
-
Premature senescence leaf mutant 86
- YY:
-
Rice japonica cultivar ‘Yunyin
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Acknowledgements
This work was supported by the National Key R&D Program Foundation of China (Grant no. 2016YFD0300508), Key program of Science and Technology in Fujian province, China (No. 2020NZ08016), and The Special Foundation of Non-Profit Research Institutes of Fujian Province (Grant no. 2018R1021-5).
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425_2021_3793_MOESM7_ESM.xlsx
Table S7 Kyoto Encyclopedia of Genes and Genomes enrichment analysis of differentially modified sites (proteins) (XLSX 65 kb)
425_2021_3793_MOESM9_ESM.xlsx
Table S9 Differentially expressed proteins and modified sites (proteins) in the carbon fixation in photosynthetic organisms pathway (map00710) (XLSX 24 kb)
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Yu, X., Zhu, Y., Xie, Y. et al. Ubiquitylomes and proteomes analyses provide a new interpretation of the molecular mechanisms of rice leaf senescence. Planta 255, 43 (2022). https://doi.org/10.1007/s00425-021-03793-z
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DOI: https://doi.org/10.1007/s00425-021-03793-z