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MicroRNA-mediated responses to colchicine treatment in barley

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Abstract

Main conclusion

In Hordeum vulgare, nine differentially expressed novel miRNAs were induced by colchicine. Five novel miRNA in colchicine solution showed the opposite expression patterns as those in water.

Abstract

Colchicine is a commonly used agent for plant chromosome set doubling. MicroRNA-mediated responses to colchicine treatment in plants have not been characterized. Here, we characterized new microRNAs induced by colchicine treatment in Hordeum vulgare using high-throughput sequencing. Our results showed that 39 differentially expressed miRNAs were affected by water treatment, including 34 novel miRNAs and 5 known miRNAs; 42 miRNAs, including 37 novel miRNAs and 5 known miRNAs, were synergistically affected by colchicine and water, and 9 differentially expressed novel miRNAs were induced by colchicine. The novel_mir69, novel_mir57, novel_mir75, novel_mir38, and novel_mir56 in colchicine treatment showed the opposite expression patterns as those in water. By analyzing these 9 differentially expressed novel miRNAs and their targets, we found that novel_mir69, novel_mir56 and novel_mir25 co-target the genes involving the DNA repair pathway. Based on our results, microRNA-target regulation network under colchicine treatment was proposed, which involves actin, cell cycle regulation, cell wall synthesis, and the regulation of oxidative stress. Overall, the results demonstrated the critical role of microRNAs mediated responses to colchicine treatment in plants.

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Abbreviations

HV0:

Barley, untreated

HV5CK:

Barley, 5 h water treatment

HV5TR:

Barley, 5 h colchicine treatment

NFYA5:

Nuclear factor YA family

TPM:

Transcripts per million

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Acknowledgements

This study was financially supported by the National Key Research and Development Program of China (2017YFD0301304) and the international Science and Technology Cooperation Projects of Anhui Province (1704e1002232) to DW, a Research Foundation for Talented Scholars from Anhui Agricultural University and the introduced leading talent research team for Universities in Anhui Province, the Natural Sciences and Engineering Research Council of Canada (RGPIN-2018-05433) to GS.

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Authors and Affiliations

  1. College of Agronomy, Anhui Agricultural University, Hefei, 230036, Anhui, China

    Fang-Yao Sun, Lin Liu, Yi Yu, Xin-Ming Ruan, Cheng-Yu Wang, Qun-Wen Hu & De-Xiang Wu

  2. Biology Department, Saint Mary’s University, Halifax, NS, B3H 3C3, Canada

    Genlou Sun

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  1. Fang-Yao Sun
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  2. Lin Liu
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Sun, FY., Liu, L., Yu, Y. et al. MicroRNA-mediated responses to colchicine treatment in barley. Planta 251, 44 (2020). https://doi.org/10.1007/s00425-019-03326-9

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