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Tissue accumulation patterns and concentrations of potassium, phosphorus, and carboxyfluorescein translocated from pine seed to the root

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Abstract

Main conclusion

Potassium (K), phosphorous (P), and carboxyfluorescein (CF) accumulate in functionally distinct tissues within the pine seedling root cortex.

Seedlings of Pinus pinea translocate exogenous CF and endogenous K and P from the female gametophyte/cotyledons to the growing radicle. Following unloading in the root tip, these materials accumulate in characteristic spatial patterns. Transverse sections of root tips show high levels of P in a circular ring of several layers of inner cortical cells. K and CF are minimal in the high P tissue. In contrast, high levels of K and CF accumulate in outer cortical cells, and in the vascular cylinder. These patterns are a property of living tissue because they change after freeze–thaw treatment, which kills the cells and results in uniform distribution of K and P. K concentration can be reduced to undetectable levels by incubation of roots in 100 mM NaCl. Inductively coupled plasma optical emission spectrometry (ICP-OES) analysis and scanning electron microscopy (SEM)/energy-dispersive X-ray spectroscopy (EDS) of root segments both reliably determine K and P concentrations.

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Abbreviations

CF:

Carboxyfluorescein

CFDA:

Carboxyfluorescein diacetate

ICP-OES:

Inductively coupled plasma optical emission spectrometry

RAM:

Root apical meristem

SEM/EDS:

Scanning electron microscopy/energy-dispersive X-ray spectroscopy

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Authors and Affiliations

  1. Biology Department, University of Louisiana, PO Box 43602, Lafayette, LA, 70504, USA

    Thomas C. Pesacreta & Karl H. Hasenstein

  2. Microscopy Center, University of Louisiana, PO Box 43602, Lafayette, LA, 70504, USA

    Thomas C. Pesacreta

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  1. Thomas C. Pesacreta
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Correspondence to Thomas C. Pesacreta.

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Pesacreta, T.C., Hasenstein, K.H. Tissue accumulation patterns and concentrations of potassium, phosphorus, and carboxyfluorescein translocated from pine seed to the root. Planta 248, 393–407 (2018). https://doi.org/10.1007/s00425-018-2897-7

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  • DOI: https://doi.org/10.1007/s00425-018-2897-7

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