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Colonic expression of calcium transporter TRPV6 is regulated by dietary sodium butyrate

  • Ion channels, receptors and transporters
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Abstract

Dietary fibers have been shown to increase the intestinal absorption of calcium (Ca2+) and magnesium (Mg2+). However, the mechanisms that explain the enhanced electrolyte absorption remain unknown. Therefore, this study aims to investigate the short-term and long-term effects of 5% (w/w) sodium butyrate (Na-butyrate), an important end-metabolite of bacterial fermentation of dietary fibers, on Ca2+ and Mg2+ homeostasis in mice. Serum Ca2+ levels were only significantly increased in mice treated with Na-butyrate for 1 day. This was associated with a twofold increase in the mRNA expression levels of Trpv6 in the proximal and distal colon. Contrary, Na-butyrate did not affect serum Mg2+ concentrations at either of the intervention periods. However, we observed a reduction in urinary Mg2+ excretion, although not significantly, after 1 day of treatment. A significant reduction of 2.5–fold in urinary Mg2+ excretion was observed after 14 days of treatment. Indeed, 14-day Na-butyrate supplementation increased colonic Trpm7 expression by 1.2-fold compared to control mice. In conclusion, short-term Na-butyrate supplementation increases serum Ca2+ levels in mice. This was associated with increased mRNA expression levels of Trpv6 in the colon, suggesting that Na-butyrate regulates the expression of genes involved in active intestinal Ca2+ absorption.

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Data availability statement

The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.

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Acknowledgements

The authors thank H. Janssen-Wagener (Radboud university medical center, Nijmegen, the Netherlands) and S. van Boxtel (Radboud University Medical Center, Nijmegen, the Netherlands) for their excellent technical support with the animal study.

Funding

This work was supported by grants from the Netherlands Organization for Scientific Research (J. Hoenderop, NWO VICI 016.130.668 and J. de Baaij, NWO VENI 016.186.012).

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Authors and Affiliations

  1. Department of Physiology, Radboud Institute for Molecular Life Sciences (RIMLS), Radboud University Medical Center (Radboudumc), P.O. Box 9101, 6500 HB, Nijmegen, the Netherlands

    Lisanne M. M. Gommers, Jenny van der Wijst, Caro Bos, Luuk A. M. Janssen, René J. M. Bindels, Jeroen H. F. de Baaij & Joost G. J. Hoenderop

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  1. Lisanne M. M. Gommers
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Contributions

L.M.M.G., J.H.Fd.B., Jvd.W., and J.G.J.H. conceived the study, and L.M.M.G., J.H.Fd.B., Jvd.W., C.B., R.J.M.B., and J.G.J.H. designed the experiments and interpreted the data. L.M.M.G., J.H.Fd.B., C.B., and L.J. performed the experiments and analyzed the data. L.M.M.G., J.H.Fd.B., Jvd.W., and J.G.J.H. wrote the paper. All authors edited and approved the final version of the manuscript.

Corresponding author

Correspondence to Joost G. J. Hoenderop.

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Ethics approval

This animal study was approved by the animal ethics board of the Radboud University Nijmegen (RU DEC 2017–0024) and by the Dutch Central Commission for Animal Experiments (CCD, AVD1030020173224).

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The authors declare no competing interests.

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Gommers, L.M.M., van der Wijst, J., Bos, C. et al. Colonic expression of calcium transporter TRPV6 is regulated by dietary sodium butyrate. Pflugers Arch - Eur J Physiol 474, 293–302 (2022). https://doi.org/10.1007/s00424-021-02648-6

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