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DNA methylation levels and telomere length in human teeth: usefulness for age estimation

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Abstract

In the last decade, increasing knowledge of epigenetics has led to the development of DNA methylation-based models to predict age, which have shown high predictive accuracy. However, despite the value of teeth as forensic samples, few studies have focused on this source of DNA. This study used bisulfite pyrosequencing to measure the methylation levels of specific CpG sites located in the ELOVL2, ASPA, and PDE4C genes, with the aim of selecting the most age-informative genes and determining their associations with age, in 65 tooth samples from individuals 15 to 85 years old. As a second aim, methylation data and measurements of relative telomere length in the same set of samples were used to develop preliminary age prediction models to evaluate the accuracy of both biomarkers together and separately in estimating age from teeth for forensic purposes. In our sample, several CpG sites from ELOVL2 and PDE4C genes, as well as telomere length, were significantly associated with chronological age. We developed age prediction quantile regression models based on DNA methylation levels, with and without telomere length as an additional variable, and adjusted for type of tooth and sex. Our results suggest that telomere length may have limited usefulness as a supplementary marker for DNA methylation-based age estimation in tooth samples, given that it contributed little improvement in the prediction errors of the models. In addition, even at older ages, DNA methylation appeared to be more informative in predicting age than telomere length when both biomarkers were evaluated separately.

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Acknowledgments

Appreciation is expressed to the Genomics ECAI of the Institute of Biomedical Research in Malaga (IBIMA), Malaga, Spain, the Pfizer-University of Granada-Andalusian Government Centre for Genomics and Oncological Research (GENYO), Granada, Spain, for their technical support, methodological expertise, and scientific advice, and K. Shashok for improving the use of English in the manuscript.

Funding

The authors also acknowledge funding from the Andalusian Centre of Excellence for Forensic Research (CEIFA).

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Authors and Affiliations

  1. Department of Forensic Medicine, Faculty of Medicine, University of Granada, Avda. de la Investigación, 11, 18016, Granada, Spain

    Ana Belén Márquez-Ruiz, Lucas González-Herrera & Aurora Valenzuela

  2. Department of Statistics, Faculty of Medicine, University of Granada, Avda. de la Investigación, 11, 18016, Granada, Spain

    Juan de Dios Luna

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  1. Ana Belén Márquez-Ruiz
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Correspondence to Ana Belén Márquez-Ruiz.

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Márquez-Ruiz, A.B., González-Herrera, L., Luna, J.d. et al. DNA methylation levels and telomere length in human teeth: usefulness for age estimation. Int J Legal Med 134, 451–459 (2020). https://doi.org/10.1007/s00414-019-02242-7

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