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TNF-alpha G/A308 polymorphism association with nasal polyposis in North part of Iran

  • Rhinology
  • Published:
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Abstract

Nasal Polyposis (NP) is a complex multi-factorial disease; associated with several environmental, genetic and inflammatory factors. TNF-alpha is one of the major pro-inflammatory cytokines involved in NP pathogenesis. Some of the polymorphisms of this gene affect its expression. Therefore, the aim of this study was to evaluate the polymorphism of TNF-alpha G/A308 gene and its association with nasal polyposis in north part of Iran. In this case-control study, 60 patients with NP and 75 healthy individuals referred to Guilan University hospital were evaluated. After DNA extraction, RFLP-PCR was used to determine polymorphism. Chi-square test was used to compare the frequency distribution of genotype and alleles of TNF-alpha gene with NP. The frequency of genotype G/G, A/A and G/A in the NP group was 46.7, 10.0 and 43.3%, and in the control group was 65.3, 5.3 and 29.3%, respectively. There was a statistically significant difference between genotype G/G in two groups (P = 0.006, OR 2.58, CI 1.27–5.23). In addition, the frequency of allele A in patients and controls was 31.7 and 20%, respectively; and this difference was statistically significant (p = 0.02). The findings of this study demonstrated that polymorphism in TNF-alpha gene might be a risk factor for NP in north part of Iran and the minor frequency of G308A allele in the current study is slightly more than other major populations. However, more investigations with high number of population are necessary in future.

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Acknowledgements

The authors would like to acknowledge vice chancellor of research, Guilan University of Medical Sciences for supporting this research.

Funding

This work is supported by the Guilan University of Medical Sciences by Grant number: 93090408, approved by University ethic committee (ethic code: 1930400407).

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Authors and Affiliations

  1. Anatomical Sciences Department, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran

    Masoumeh Faghani, Babak Pourgholamali & Farshad Moharami

  2. Cellular and Molecular Research Center, Faculty of Medicine, Guilan University of Medical Sciences, Rasht, Iran

    Masoumeh Faghani, Parvaneh Keshavars & Alireza Sharafshah

  3. Rino-sinus, Ear, and Skull base Diseases Research Center, Amir-al Momenin University Hospital, Guilan University of Medical Sciences, 17 Shahrivar Ave, Rasht, Guilan, Iran

    Shadman Nemati

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  1. Masoumeh Faghani
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  2. Parvaneh Keshavars
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  3. Alireza Sharafshah
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  4. Babak Pourgholamali
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Correspondence to Shadman Nemati.

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The authors declare that there was no any conflict of Interest during performing this study.

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Faghani, M., Keshavars, P., Sharafshah, A. et al. TNF-alpha G/A308 polymorphism association with nasal polyposis in North part of Iran. Eur Arch Otorhinolaryngol 275, 2253–2257 (2018). https://doi.org/10.1007/s00405-018-5059-4

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  • DOI: https://doi.org/10.1007/s00405-018-5059-4

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