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LncRNA SNHG14 accelerates breast cancer progression through sponging miR-543 and regulating KLF7 expression

  • Gynecologic Oncology
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Purpose

Dysregulation of long non-coding RNAs (lncRNAs) is being found to have relevance to human cancers, including breast cancer (BC). The aim of this study was to further explore the functional role and molecular mechanisms of small nucleolar RNA host gene 14 (SNHG14) on BC progression.

Methods

The expression levels of SNHG14, miR-543, and krüppel-like factor 7 (KLF7) mRNA were determined by quantitative real-time PCR. Western blot analysis was used to evaluate KLF7 protein level. Cell proliferation, apoptosis, and migration and invasion abilities were detected by Cell Counting kit-8 assay, flow cytometry, and transwell assay, respectively. The direct interactions between miR-543 and SNHG14 or KLF7 were confirmed using dual-luciferase reporter assays.

Results

Our data indicated that SNHG14 expression was increased in BC tissues and cells, and SNHG14 knockdown mitigated the proliferation, migration, and invasion and facilitated apoptosis of BC cells. SNHG14 directly interacted with miR-543. MiR-543 mediated the regulatory effects of SNHG14 silencing on BC cell behaviors. Moreover, KLF7 was a direct target of miR-543. Overexpressed miR-543-mediated anti-proliferation, anti-migration, anti-invasion, and pro-apoptosis effects were mediated by KLF7. Furthermore, SNHG14 modulated KFL7 expression through acting as a competing endogenous RNA (ceRNA) of miR-543 in BC cells.

Conclusion

Our study suggested that SNHG14 knockdown hindered BC progression in vitro at least partly through acting as a ceRNA of miR-543 and modulating KLF7 expression, providing evidence for SNHG14 as a potential target for BC therapy.

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Authors and Affiliations

  1. Department of Laboratory Medicine, Jingmen No. 1 People’s Hospital, No.6 Zhongtian Street, Dongbao District, Jingmen, 448000, Hubei, China

    Daoping Zhang, Xu Ding & Meirong Peng

Authors
  1. Daoping Zhang
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  2. Xu Ding
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  3. Meirong Peng
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Contributions

DZ: project development, data collection, data analysis, and manuscript writing. XD: data collection and data analysis. MP: project development, data analysis, and manuscript editing.

Corresponding author

Correspondence to Meirong Peng.

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Conflict of interest

The authors have no interests to disclose.

Ethical approval

Our study was approved by the Human Research Ethics Committee of Jingmen No. 1 People’s Hospital, and written informed consent was signed by each participator before surgery.

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Cite this article

Zhang, D., Ding, X. & Peng, M. LncRNA SNHG14 accelerates breast cancer progression through sponging miR-543 and regulating KLF7 expression. Arch Gynecol Obstet 305, 1507–1516 (2022). https://doi.org/10.1007/s00404-021-06300-7

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  • DOI: https://doi.org/10.1007/s00404-021-06300-7

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