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Noninvasive assays of in vitro matured human oocytes showed insignificant correlation with fertilization and embryo development

  • Gynecologic Endocrinology and Reproductive Medicine
  • Published:
Archives of Gynecology and Obstetrics Aims and scope Submit manuscript

Abstract

Purpose

Recently, the upgrading of in vitro maturation (IVM) of human oocytes as a promising strategy has emerged in assisted reproductive technology (ART). The goal was to evaluate the correlation of the in vitro matured oocytes selected on the basis of the zona pellucida (ZP) birefringence and meiotic spindles (MS) detection with fertilization and subsequent embryo development in ICSI program.

Methods

A total of 168 immature oocytes [germinal vesicle (n = 140) and metaphase I (n = 28)] obtained from patients undergoing oocytes retrieval for ICSI. After in vitro culture for 24–40 h, 112 (67 %) oocytes reached to MII stage. Using a polarized microscopy, the presence of MS and ZP birefringence were assessed in matured oocytes, followed by ICSI performance.

Results

The rates of fertilization in oocytes with spindles (51.3 %) were similar to that of the oocytes without spindles (50.7 %; P = 1.00). Moreover, the fertilization rates in high birefringence (HB) oocytes was not statistically different than oocytes with low birefringence (LB) (P = 0.44). The findings also showed that 64.9 % of the fertilized oocytes developed to embryos, in which 33.3 % were derived from spindle-detected oocytes. Regarding the ZP birefringence, 35.5 % of the embryos were derived from HB oocytes.

Conclusions

There were insignificant relationships between the MS detection and ZP birefringence score with the rates of fertilization and embryo development in IVM oocytes.

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Acknowledgments

The authors would like to thank Dr. Nasim Tabibnejad for her English editing of the manuscript.

Conflict of interest

None.

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Authors and Affiliations

  1. Research and Clinical Center for Infertility, Shahid Sadoughi University of Medical Sciences, Yazd, Iran

    Sareh Ashourzadeh, Mohammad Ali Khalili, Marjan Omidi, Seyed Mehdi Kalantar & Abbas Aflatoonian

  2. Afzalipour Clinical Center for Infertility, Afzalipour Hospital, Kerman University of Medical Sciences, Kerman, Iran

    Sareh Ashourzadeh & Victoria Habibzadeh

  3. Department of Anatomical Sciences, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran

    Seyed Nooraldin Nematollahi Mahani

Authors
  1. Sareh Ashourzadeh
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  2. Mohammad Ali Khalili
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  3. Marjan Omidi
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  4. Seyed Nooraldin Nematollahi Mahani
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  5. Seyed Mehdi Kalantar
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  6. Abbas Aflatoonian
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  7. Victoria Habibzadeh
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Correspondence to Mohammad Ali Khalili.

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Ashourzadeh, S., Khalili, M.A., Omidi, M. et al. Noninvasive assays of in vitro matured human oocytes showed insignificant correlation with fertilization and embryo development. Arch Gynecol Obstet 292, 459–463 (2015). https://doi.org/10.1007/s00404-015-3644-y

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  • DOI: https://doi.org/10.1007/s00404-015-3644-y

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