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Houttuynia cordata aqueous extract attenuated glycative and oxidative stress in heart and kidney of diabetic mice

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European Journal of Nutrition Aims and scope Submit manuscript

An Erratum to this article was published on 22 April 2017

Abstract

Purpose

The anti-glycative and anti-oxidative effects from Houttuynia cordata leaves aqueous extract (HCAE) in heart and kidney of diabetic mice were examined.

Methods

HCAE, at 1 or 2 %, was supplied in drinking water for 8 weeks. Plasma glucose and blood urea nitrogen (BUN) levels and creatine phosphokinase (CPK) activity were measured. The production of oxidative and inflammatory factors was determined. Activity and protein expression of associated enzymes or regulators were analyzed.

Results

HCAE intake at both doses lowered plasma glucose and BUN levels, and CPK activity and also restored creatinine clearance rate in diabetic mice. HCAE intake, only at 2 %, retained plasma insulin levels (P < 0.05). HCAE reduced reactive oxygen species, protein carbonyl, interleukin-6, tumor necrosis factor-alpha, N ε-(carboxymethyl)-lysine, pentosidine and fructose levels, and reserved glutathione content in heart and kidney of diabetic mice (P < 0.05). Diabetes enhanced aldose reductase (AR) activity and protein expression in heart and kidney (P < 0.05). HCAE intake at both doses decreased renal AR activity and protein expression, but only at 2 % lowered cardiac AR activity and protein expression (P < 0.05). Diabetes increased protein expression of RAGE, p47phox and gp91phox, nuclear factor kappa-B (NF-κB) p50, NF-κB p65 and mitogen-activated protein kinase in heart and kidney (P < 0.05). HCAE intake only at 2 % limited RAGE expression, but at 1 and 2 % downregulated p47phox, NF-κB p65 and p-p38 expression in these organs (P < 0.05).

Conclusions

These findings suggest that Houttuynia cordata leaves aqueous extract could ameliorate cardiac and renal injury under diabetic condition.

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Abbreviations

AGEs:

Advanced glycation endproducts

AR:

Aldose reductase

BUN:

Blood urea nitrogen

CCr:

Creatinine clearance rate

CML:

N ε-(carboxymethyl)-lysine

CPK:

Creatine phosphokinase

Cr:

Creatinine

DCFH-DA:

2′,7′-Dichlorofluorescein diacetate

DNP:

Dinitrophenylhydrazine

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

GSH:

Glutathione

HCAE:

Houttuynia cordata leaves aqueous extract

IL:

Interleukin

MAPK:

Mitogen-activated protein kinase

NF-κB:

Nuclear factor kappa-B

RFU:

Relative fluorescence unit

ROS:

Reactive oxygen species

TNF-alpha:

Tumor necrosis factor-alpha

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Acknowledgments

This study was partially supported by a Grant from Ministry of Science and Technology, Taipei City, Taiwan (NSC 102-2313-B-039-002-MY3).

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Authors and Affiliations

  1. School of Nutrition, Chung Shan Medical University, Taichung City, Taiwan

    Cheng-chin Hsu

  2. Department of Nutrition, China Medical University, 91, Hsueh-shih Rd., Taichung City, Taiwan

    Hui-ting Yang, Jing-jing Ho & Mei-chin Yin

  3. Department of Health and Nutrition Biotechnology, Asia University, Taichung City, Taiwan

    Mei-chin Yin

  4. School of Pharmacy, China Medical University, Taichung City, Taiwan

    Jen-ying Hsu

  5. Department of Nutrition, Chung Shan Medical University Hospital, Taichung City, Taiwan

    Cheng-chin Hsu

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  1. Cheng-chin Hsu
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  2. Hui-ting Yang
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Correspondence to Mei-chin Yin.

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There was no conflict of interest regarding this manuscript.

Additional information

An erratum to this article is available at http://dx.doi.org/10.1007/s00394-017-1463-6.

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Hsu, Cc., Yang, Ht., Ho, Jj. et al. Houttuynia cordata aqueous extract attenuated glycative and oxidative stress in heart and kidney of diabetic mice. Eur J Nutr 55, 845–854 (2016). https://doi.org/10.1007/s00394-015-0994-y

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  • DOI: https://doi.org/10.1007/s00394-015-0994-y

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