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Genome-wide copy number changes and CD133 expression characterized distinct subset of colon polyps: differentiation between incidental polyps and cancer-associated polyps

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International Journal of Colorectal Disease Aims and scope Submit manuscript

Abstract

Purpose

Colorectal polyps are generally believed to be the precursors of colorectal cancers (CRC); however, the proportion and speed of progression differed widely in different subsets of polyps. Using microarray-based comparative genomic hybridization (aCGH) platform and CD133 immunostaining, we characterized colon polyps according to their association with CRC that developed in the same individual.

Patients and methods

aCGH was performed to unveil genomic changes in 18 cancer-synchronous polyps (CSP), and 9 cancer-preceding polyps (CPP), together with their corresponding cancers and 16 cases of incidental polyps (IP), were examined for comparison. aCGH profiles were analyzed to determine the clonal relationship (CR) between the paired adenoma and carcinoma. CD133 expressions in each subset of polyps were quantified by immunohistochemistry (IHC) staining.

Results

Progressive genomic changes were observed from IP, CSP/CPP to CRC; they encompass an entire chromosomal region in IP and sub-chromosomal region in CSP/CPP and CRC. CR analyses demonstrated that 50 % of CSP and 67 % of CPP were clonally related to the concurrent or later developed carcinomas, respectively. The CD133 expression levels were significantly higher in CSP/CPP than those in IP (P < 0.0001) and even higher in CSP/CPP that were clonally related to their corresponding carcinomas than CSP/CPP that were unrelated (P < 0.05).

Conclusions

There were more genomic changes in CSP/CPP than IP; more than half of the CSP/CPP were clonally related to the corresponding carcinomas. Genomic changes at sub-chromosomal regions and/or high CD133 expression were associated with CSP/CPP and highlighted their carcinogenic potential.

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Acknowledgments

We thank Dr. Li-Li Li and Felicia Hsin-Yu Jiang for language revision of the manuscript. This work was supported by research grants MOST 104-2319-B-010-001, NSC97-3112-B-075-002, NSC98-3112-B-010-023-B4, and NSC 97-2320-B-010-024-MY3 from National Science Council and Aim for the Top University Plan from the Ministry of Education, Taiwan.

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The authors declare that they have no competing interests.

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    Authors and Affiliations

    1. Department of Education and Research, Taipei City Hospital, Taipei, Taiwan

      Chih-Yung Yang & Chi-Hung Lin

    2. Institute of Microbiology and Immunology, National Yang-Ming University, Taipei, Taiwan

      Ju-Yu Tseng & Chi-Hung Lin

    3. VYM Genome Research Center, National Yang-Ming University, Taipei, Taiwan

      Chian-Feng Chen, Chia-Ling Hua & Chi-Hung Lin

    4. Department of Pathology, Taipei Veterans General Hospital, Taipei, Taiwan

      Teh-Ying Chou

    5. Department of Pathology, Tri-Service General Hospital, Taipei, Taiwan

      Hong-Wei Gao

    6. School of Medicine, National Yang-Ming University, Taipei, Taiwan

      Chi-Hung Lin, Jen-Kou Lin & Jeng-Kai Jiang

    7. Division of Colon & Rectal Surgery, Department of Surgery, Taipei Veterans General Hospital, Pei-Tou, Taipei, 11217, Taiwan

      Jen-Kou Lin & Jeng-Kai Jiang

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    Chih-Yung Yang, Ju-Yu Tseng and Chian-Feng Chen contributed equally to this work.

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    Yang, CY., Tseng, JY., Chen, CF. et al. Genome-wide copy number changes and CD133 expression characterized distinct subset of colon polyps: differentiation between incidental polyps and cancer-associated polyps. Int J Colorectal Dis 30, 1617–1626 (2015). https://doi.org/10.1007/s00384-015-2319-2

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