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Is maintenance of the ileocecal valve important to the intestinal adaptation mechanisms in a weaning rat model of short bowel?

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Abstract

Purpose

To evaluate the role of maintenance of the ileocecal valve (ICV) in intestinal adaptation mechanisms, in a weaning rat experimental model of short bowel.

Methods

Forty animals were operated on to produce short bowel syndrome. They were divided into five groups: maintenance (MV) or resection of ICV (RV), kill after 4 days (MV4 and RV4) or 21 days (MV21 and RV21), and a control group (21-day-old rats). Body weights, small bowel and colon lengths and diameters, villus heights, crypt depths, lamina propria and muscle layer thickness, as well as the apoptosis index of villi and crypts and expression of pro- and anti-apoptotic genes, were studied.

Results

Preservation of the ICV promoted increased weight gain (p = 0.0001) and intestinal villus height after 21 days; crypt depth was higher in comparison to controls. It was verified a higher expression of Ki-67 in bowel villi and crypts (p = 0.018 and p = 0.015, respectively) in RV4 group and a higher expression in bowel villi of MV4 group animals (p = 0.03). The maintenance of ICV promoted late increased expression of the anti-apoptotic gene Bcl-XL in the colon (p = 0.043, p = 0.002, p = 0.01).

Conclusion

The maintenance of the ICV led to positive changes in this model.

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Authors and Affiliations

  1. Pediatric Surgery Division, Pediatric Liver Transplantation Unit and Laboratory of Research in Pediatric Surgery (LIM 30), University of Sao Paulo Medical School, Sao Paulo, Brazil

    Guilherme Garcia Barros, Ana Cristina Aoun Tannuri, Ítalo Gerardo Rotondo, Vitor Van Vaisberg, Leandro Silveira Sarmento, Cícero Mendes Neto, Suellen Serafini, Josiane de Oliveira Gonçalves, Maria Cecília Mendonça Coelho & Uenis Tannuri

  2. Faculdade de Medicina da Universidade de São Paulo, Avenida Dr. Arnaldo 455, 4o andar sala 4109, CEP 01246-903, São Paulo, SP, Brazil

    Uenis Tannuri

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  1. Guilherme Garcia Barros
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  2. Ana Cristina Aoun Tannuri
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  3. Ítalo Gerardo Rotondo
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  4. Vitor Van Vaisberg
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  5. Leandro Silveira Sarmento
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Correspondence to Uenis Tannuri.

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All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Barros, G.G., Tannuri, A.C.A., Rotondo, Í.G. et al. Is maintenance of the ileocecal valve important to the intestinal adaptation mechanisms in a weaning rat model of short bowel?. Pediatr Surg Int 34, 1215–1224 (2018). https://doi.org/10.1007/s00383-018-4333-2

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  • DOI: https://doi.org/10.1007/s00383-018-4333-2

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