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Radioimmunoassay of motilin

Validation and Studies on the Relationship between Plasma Motilin and Interdigestive Myoelectric Activity of the duodenum of dog

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Abstract

A radioimmunoassay method of motilin was developed in our laboratory and was validated in dogs with a platinum monopolar electrode in the duodenum. We confirmed that a bolus infusion of 0.3 M tris-buffer solution or 0.1 N HCl solution in the duodenum produces a significant rise in plasma immunoreactive motilin (IRM) concentrations. This coincided with a marked increase in the percentage of spike potentials on slow waves of the duodenum, similar to phase III of interdigestive myoelectric-activity (MA). A possible relationship between plasma IRM and interdigestive MA of canine duodenum was studied. It was found that cyclic changes occurred in the fasting plasma IRM concentrations in dogs. While the peak motilin concentration was always observed in phase III, the lowest concentration of motilin was found in phase I of interdigestive MA in the duodenum. In dogs with the electrodes in the duodenum and jejunum, the peak IRM concentration did not correlate with phase III of interdigestive MA in the jejunum. A dose of synthetic porcine motilin, 0.06 μg/kg/hr, which produced the plasma IRM concentration comparable to the peak fasting motilin concentration, could induce an identical phase III in the duodenum. These observations indicate that there is a relationship between cyclic changes in plasma IRM concentrations and interdigestive MA of the duodenum. It is suggested further that motilin is a hormone which may play an important role in inducing phase III of interdigestive MA in the duodenum.

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Authors and Affiliations

  1. Isaac Gordon Center for Digestive Diseases and Nutrition, Department of Medicine, the Genesee Hospital, 224 Alexander Street, 14607, Rochester, New York

    Kae Yol Lee MD,  William Y. Chey MD, Hsin-Hsiung Tai PhD, Haruaki Yajima PhD & David Wagner BS

  2. the University of Rochester School of Medicine and Dentistry, Rochester, New York

    Kae Yol Lee MD,  William Y. Chey MD, Hsin-Hsiung Tai PhD, Haruaki Yajima PhD & David Wagner BS

  3. University of Kyoto, Japan

    Kae Yol Lee MD,  William Y. Chey MD, Hsin-Hsiung Tai PhD, Haruaki Yajima PhD & David Wagner BS

Authors
  1. Kae Yol Lee MD
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  2. William Y. Chey MD
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  3. Hsin-Hsiung Tai PhD
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  4. Haruaki Yajima PhD
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  5. David Wagner BS
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Additional information

This work was supported by the Gastrointestinal Research Fund at The Genesee Hospital.

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Lee, K.Y., Chey, W.Y., Tai, HH. et al. Radioimmunoassay of motilin. Digest Dis Sci 23, 789–795 (1978). https://doi.org/10.1007/BF01079787

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