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Involvement of the crustacean hyperglycemic hormone (CHH) in the physiological compensation of the freshwater crayfish Cherax quadricarinatus to low temperature and high salinity stress

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Abstract

This study was aimed at determining the role of the crustacean hyperglycemic hormone (CHH) in the physiological compensation to both saline and thermal stress, in the freshwater crayfish Cherax quadricarinatus. By determining the expression of the CHH gene in the eyestalk of juvenile crayfish, we found that maximal induction of CHH was induced at high salinity (10 g/L) and low temperature (20 °C). In order to investigate the role of CHH in the physiological compensation to such stressful conditions, recombinant CHH was supplied to stressed animals. CHH-injected crayfish showed increased hemolymphatic levels of glucose, in accordance with a significant utilization of glycogen reserves from the hepatopancreas. Furthermore, CHH administration allowed stressed animals to regulate hemolymphatic sodium and potassium at more constant levels than controls. Taken together, these results suggest a relevant role of CHH in increasing the energy available intended for processes involved in the physiological compensation of C. quadricarinatus to both saline and thermal stress.

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Acknowledgments

This work was supported by grants from both the University of Buenos Aires UBACYT (2012–2015 program, code 20020110100044) and CONICET (PIP 2010–2012, code 100884). The authors wish to thank Dr. Prof. Amir Sagi for his generous gift of the CHH cDNA, Dr. Juan Gerez for his valuable help with the analysis of CHH expression, and Amir Dyzenchauz for revising the English style.

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Authors and Affiliations

  1. Department of Biodiversity and Experimental Biology, FCEN, University of Buenos Aires, Buenos Aires, Argentina

    Natalia C. Prymaczok, Valeria M. Pasqualino, Verónica E. Viau, Enrique M. Rodríguez & Daniel A. Medesani

  2. Institute of Biodiversity, Experimental and Applied Biology (IBBEA), CONICET-UBA, Ciudad Universitaria, Pab. II, C1428EGA, Buenos Aires, Argentina

    Verónica E. Viau, Enrique M. Rodríguez & Daniel A. Medesani

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  1. Natalia C. Prymaczok
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  2. Valeria M. Pasqualino
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  3. Verónica E. Viau
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  4. Enrique M. Rodríguez
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  5. Daniel A. Medesani
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Correspondence to Enrique M. Rodríguez.

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Communicated by H. V. Carey.

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Prymaczok, N.C., Pasqualino, V.M., Viau, V.E. et al. Involvement of the crustacean hyperglycemic hormone (CHH) in the physiological compensation of the freshwater crayfish Cherax quadricarinatus to low temperature and high salinity stress. J Comp Physiol B 186, 181–191 (2016). https://doi.org/10.1007/s00360-015-0954-0

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