Skip to main content
SpringerLink
Log in

Polymorphisms of clip domain serine proteinase and serine proteinase homolog in the swimming crab Portunus trituberculatus and their association with Vibrio alginolyticus

  • Biology
  • Published:
Chinese Journal of Oceanology and Limnology Aims and scope Submit manuscript

Abstract

Clip domain serine proteases (cSPs) and their homologs (SPHs) play an important role in various biological processes that are essential components of extracellular signaling cascades, especially in the innate immune responses of invertebrates. Here, polymorphisms of PtcSP and PtSPH from the swimming crab Portunus trituberculatus were investigated to explore their association with resistance/susceptibility to Vibrio alginolyticus. Polymorphic loci were identified using Clustal X, and characterized with SPSS 16.0 software, and then the significance of genotype and allele frequencies between resistant and susceptible stocks was determined by a χ 2 test. A total of 109 and 77 single nucleotide polymorphisms (SNPs) were identified in the genomic fragments of PtcSP and PtSPH, respectively. Notably, nearly half of PtSPH polymorphisms were found in the non-coding exon 1. Fourteen SNPs investigated were significantly associated with susceptibility/resistance to V. alginolyticus (P <0.05). Among them, eight SNPs were observed in introns, and one synonymous, four non-synonymous SNPs and one ins-del were found in coding exons. In addition, five simple sequence repeats (SSRs) were detected in intron 3 of PtcSP. Although there was no statistically significant difference of allele frequencies, the SSRs showed different polymorphic alleles on the basis of the repeat number between resistant and susceptible stocks. After further validation, polymorphisms investigated here might be applied to select potential molecular markers of P. trituberculatus with resistance to V. alginolyticus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bao Y B, Li L, Zhang G F. 2010. Polymorphism of the superoxide dismutase gene family in the bay scallop (Argopecten irradians) and its association with resistance/ susceptibility to Vibrio anguillarum. Developmental & Comparative Immunology, 34 (5): 553–561.

    Article  Google Scholar 

  • Chen J M, Sun Y X. 2011. Variation in the analysis of positively selected sites using nonsynonymous/synonymous rate ratios: an example using influenza virus. PLoS One, 6 (5): e19996.

    Article  Google Scholar 

  • Cui Z X, Liu Y, Wu D H, Luan W S, Wang S Y, Li Q Q, Song C W. 2010. Molecular cloning and characterization of a serine proteinase homolog prophenoloxidase-activating factor in the swimming crab Portunus trituberculatus. Fish & Shellfish Immunology, 29 (4): 679–686.

    Article  Google Scholar 

  • Dekkers J C M, Hospital F. 2002. The use of molecular genetics in the improvement of agricultural populations. Nature Reviews Genetics, 3 (1): 22–32.

    Article  Google Scholar 

  • Duan J B, Wainwright M S, Comeron J M, Saitou N, Sanders A R, Gelernter J, Gejman P V. 2003. Synonymous mutations in the human dopamine receptor D2 (DRD2) affect mRNA stability and synthesis of the receptor. Human Molecular Genetics, 12 (3): 205–216.

    Article  Google Scholar 

  • Hancock J M. 1995. The contribution of slippage-like processes to genome evolution. Journal of Molecular Evolution, 41 (6): 1038–1047.

    Article  Google Scholar 

  • Hao G J, Lin F, Mu C K, Li R H, Yao J Y, Yuan X M, Pan X Y, Shen J Y, Wang C L. 2015. SNP E4-205 C/T in C-type lectin of Portunus trituberculatus is association with susceptibility/resistance to Vibrio alginolyticus challenge. Aquaculture, 442: 125–131.

    Article  Google Scholar 

  • Hershberg R, Petrov D A. 2008. Selection on codon bias. Annual Review of Genetics, 42 (1): 287–299.

    Article  Google Scholar 

  • Li L, Zhao J M, Wang L L, Qiu L M, Zhang H, Dong C H, Cong M, Song L S. 2009. The polymorphism of lysozyme gene in Zhikong scallop (Chlamys farreri) and its association with susceptibility/resistance to Listonella anguillarum. Fish & Shellfish Immunology, 27 (2): 136–142.

    Article  Google Scholar 

  • Li X H, Cui Z X, Liu Y, Song C W, Shi G H, Wang C L. 2013. Polymorphisms of anti-lipopolysaccharide factors in the swimming crab Portunus trituberculatus and their association with resistance/susceptibility to Vibrio alginolyticus. Fish & Shellfish Immunology, 34 (6): 1560–1568.

    Article  Google Scholar 

  • Liu Q, Wang X Z, Dai F Y, Liu P, Li J. 2007. Preliminary study on Vibrio alginolyticus disease in Portunus trituberculatus. Shandong Fish, 24 (9): 1–3. (in Chinese with English abstract)

    Google Scholar 

  • Liu Z J, Cordes J F. 2004. DNA marker technologies and their applications in aquaculture genetics. Aquaculture, 238 (1-4): 1–37.

    Article  Google Scholar 

  • Liu Z J. 2007. Single nucleotide polymorphism (SNP). In: Liu Z J ed. Aquaculture Genome Technologies. Blackwell, USA. p.59–72.

    Chapter  Google Scholar 

  • Pagani F, Baralle F E. 2004. Genomic variants in exons and introns: identifying the splicing spoilers. Nature Reviews Genetics, 5 (5): 389–396.

    Article  Google Scholar 

  • Song C W, Cui Z X, Liu Y, Li Q Q, Li X H, Shi G H, Wang C L. 2013a. Characterization and functional analysis of serine proteinase and serine proteinase homologue from the swimming crab Portunus trituberculatus. Fish & Shellfish Immunology, 35 (2): 231–239.

    Article  Google Scholar 

  • Song C W, Luo D L, Cui Z X, Liu Y, Li X H, Shi G H, Wang C L. 2013b. Polymorphism of crustins in the swimming crab (Portunus trituberculatus) and its association with Vibrio alginolyticus. Aquaculture Research, 46 (5): 1261–1268.

    Article  Google Scholar 

  • Thanh N M, Barnes A C, Mather P B, Li Y T, Lyons R E. 2010. Single nucleotide polymorphisms in the actin and crustacean hyperglycemic hormone genes and their correlation with individual growth performance in giant freshwater prawn Macrobrachium rosenbergii. Aquaculture, 301 (1-4): 7–15.

    Article  Google Scholar 

  • Vignal A, Milan D, San Cristobal M, Eggen A. 2002. A review on SNP and other types of molecular markers and their use in animal genetics. Genetics Selection Evolution, 34: 275.

    Article  Google Scholar 

  • Wan X H, Shen H, Wang L B, Cheng Y X. 2011. Isolation and characterization of Vibrio metschnikovii causing infection in farmed Portunus trituberculatus in China. Aquaculture International, 19 (2): 351–359.

    Article  Google Scholar 

  • Wang G L, Jin S, Chen Y E, Li Z. 2006. Study on pathogens and pathogenesis of emulsification disease of Portunus trituberculatus. Advances in Marine Science, 24 (4): 526–531. (in Chinese with English abstract)

    Google Scholar 

  • Wang L, Su J G, Yang C R, Wan Q Y, Peng L M. 2012. Genomic organization, promoter activity of grass carp MDA5 and the association of its polymorphisms with susceptibility/ resistance to grass carp reovirus. Mol ecular Immunol ogy, 50 (4): 236–243.

    Article  Google Scholar 

  • Yue X, Wang H X, Huang X H, Wang C, Chai X L, Wang C D, Liu B Z. 2012. Single nucleotide polymorphisms in i-type lysozyme gene and their correlation with vibrio-resistance and growth of clam Meretrix meretrix based on the selected resistance stocks. Fish & Shellfish Immunology, 33 (3): 559–568.

    Article  Google Scholar 

  • Zeng D G, Chen X H, Li Y M et al. 2008. Analysis of Hsp70 in Litopenaeus vannamei and detection of SNPs. Journal of Crustacean Biology, 28 (4): 727–730.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. College of Fisheries and Life Science, Dalian Ocean University, Dalian, 116023, China

    Meng Liu  (刘盟)

  2. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China

    Meng Liu  (刘盟), Yuan Liu  (刘媛), Min Hui  (惠敏), Chengwen Song  (宋呈文) & Zhaoxia Cui  (崔朝霞)

  3. Laboratory for Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266071, China

    Zhaoxia Cui  (崔朝霞)

  4. National & Local Joint Engineering Laboratory of Ecological Mariculture, Qingdao, 266071, China

    Zhaoxia Cui  (崔朝霞)

Authors
  1. Meng Liu  (刘盟)
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Yuan Liu  (刘媛)
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Min Hui  (惠敏)
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Chengwen Song  (宋呈文)
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Zhaoxia Cui  (崔朝霞)
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhaoxia Cui  (崔朝霞).

Additional information

Supported by the National Natural Science Foundation of China (Nos. 41206147, 31302187) and the Scientific and Technological Innovation Project Financially Supported by Qingdao National Laboratory for Marine Science and Technology (No. 2015ASKJ02)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Liu, M., Liu, Y., Hui, M. et al. Polymorphisms of clip domain serine proteinase and serine proteinase homolog in the swimming crab Portunus trituberculatus and their association with Vibrio alginolyticus . Chin. J. Ocean. Limnol. 35, 235–243 (2017). https://doi.org/10.1007/s00343-016-5331-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00343-016-5331-7

Keywords

Search

Navigation