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Interspecific competition and allelopathic interaction between Karenia mikimotoi and Dunaliella salina in laboratory culture

  • S2 Adaptation and Evolution to Special Environment of Coastal Zone
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Abstract

Algal allelopathy is a manifold ecological/physiological phenomenon that is focused on chemical interactions and autotoxicity. We investigated the allelopathic interactions between Karenia mikimotoi and Dunaliella salina in laboratory cultures based on diff erent temperature (15°C, 20°C, and 25°C) and lighting (40, 80, and 160 μmol/(m2·s)) conditions. The growth of D. salina in bi-algae culture (1:1 size/density) was significantly restrained. The results of cell-free filtrate culture indicate that direct cell-tocell contact was not necessary in interspecific competition. Further experimental results demonstrated that allelochemicals released from K. mikimotoi were markedly influenced by both temperature (P =0.013) and irradiance (P =0.003), resulting in diff erent growth characteristics of D. salina in filtrate mediums. Compared with the plateau period, K. mikimotoi exudates in the exponential phase had a stronger short-term inhibition effect on D. salina in normal conditions. A clear concentration-dependent relationship was observed in the effect of allelochemicals released from K. mikimotoi with low-promoting and high-repressing effects on D. Salina in a short time-scale. In addition, allelopathic substances remain stable and effective under high temperature and pressure stress. Many flocculent sediments adhering with D. salina cells were observed in all filtrate mediums, while the quantity and color depended on the original culture conditions.

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Authors and Affiliations

  1. Ocean University of China, Qingdao, 266071, China

    Dong He  (何冬), Jiao Liu  (刘娇), Bin Zhou  (周斌) & Xuexi Tang  (唐学玺)

  2. State Key Laboratory of Satellite Ocean Environmen Dynamics, Second Institute of Oceanography, State Oceanic Administration (SOA), Hangzhou, 310012, China

    Dong He  (何冬), Qiang Hao  (郝锵) & Lihua Ran  (冉莉华)

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  1. Dong He  (何冬)
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  2. Jiao Liu  (刘娇)
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Correspondence to Xuexi Tang  (唐学玺).

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Supported by the State Key Laboratory of Satellite Ocean Environment Dynamics (Second Institute of Oceanography, SOA) (No. SOED1418), the Public Science and Technology Research Funds Projects of Ocean (No. 201305027), the National Natural Science Foundation of China (No. 91128212), and the Research Fund for the Doctoral Program of Higher Education of China (No. 20110132120025)

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He, D., Liu, J., Hao, Q. et al. Interspecific competition and allelopathic interaction between Karenia mikimotoi and Dunaliella salina in laboratory culture. Chin. J. Ocean. Limnol. 34, 301–313 (2016). https://doi.org/10.1007/s00343-016-4320-1

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