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Comparative genomics of N-acetyl-5-methoxytryptamine members in four Prunus species with insights into bud dormancy and abiotic stress responses in Prunus avium

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Abstract

Key message

This study provides novel insights into the evolution, diversification, and functions of melatonin biosynthesis genes in Prunus species, highlighting their potential role in regulating bud dormancy and abiotic stresses.

Abstract

The biosynthesis of melatonin (MEL) in plants is primarily governed by enzymatic reactions involving key enzymes such as serotonin N-acetyltransferase (SNAT), tryptamine 5-hydroxylase (T5H), N-acetylserotonin methyltransferase (ASMT) and tryptophan decarboxylase (TDC). In this study, we analyzed Melatonin genes in four Prunus species such as Prunus avium (Pavi), Prunus pusilliflora (Ppus), Prunus serulata (Pser), and Prunus persica (Pper) based on comparative genomics approach. Among the four Prunus species, a total of 29 TDCs, 998 T5Hs, 16 SNATs, and 115 ASMTs within the genome of four Prunus genomes. A thorough investigation of melatonin-related genes was carried out using systematic biological methods and comparative genomics. Through phylogenetic analysis, orthologous clusters, Go enrichment, syntenic relationship, and gene duplication analysis, we discovered both similarities and variations in Melatonin genes among these Prunus species. Additionally, our study revealed the existence of unique subgroup members in the Melatonin genes of these species, which were distinct from those found in Arabidopsis genes. Furthermore, the transcriptomic expression analysis revealed the potential significance of melatonin genes in bud dormancy regulation and abiotic stresses. Our extensive results offer valuable perspectives on the evolutionary patterns, intricate expansion, and functions of PavMEL genes. Given their promising attributes, PavTDCs, PavT5H, PavNAT, and three PavASMT genes warrant in-depth exploration as prime candidates for manipulating dormancy in sweet cherry. This was done to lay the foundation for future explorations into the structural and functional aspects of these factors in Prunus species. This study offers significant insights into the functions of ASMT, SNAT, T5H, and TDC genes and sheds light on their roles in Prunus avium. Moreover, it established a robust foundation for further exploration functional characterization of melatonin genes in fruit species.

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Data availability

The datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found in the article/ Supplementary Material.

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Funding

This study was funded by Shanghai Agriculture Applied Technology Development Program, China (Grant No. 2022–02-08–00-12-F01120), Natural Science Foundation of Shanghai (Grant No. 23ZR1430600), Shanghai Agriculture Applied Technology Development Program, China (Grant No. 2022–02-08–00-12-F01111), China Agriculture Research System (Grant No. CARS-30–2-08), National Natural Science Foundation of China (Grant No. 32102347), Shanghai Sailing Program (Grant No. 21YF1422100), Startup Fund for Young Faculty at SJTU (Grant No. 21X010500643).

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Authors and Affiliations

  1. Department of Plant Science, School of Agriculture and Biology, Shanghai Jiao Tong University, Minhang District Jianchuan Road No.601, Shanghai, 200240, People’s Republic of China

    Muhammad Aamir Manzoor, Yan Xu, Zhengxin lv, Jieming Xu, Yuxuan Wang, Wanxia Sun, Xunju Liu, Li Wang, Ruie Liu, Songtao Jiu & Caixi Zhang

  2. Queensland Alliance of Agriculture and Food Innovation, The University of Queensland, Brisbane, 4072, Australia

    Muhammad Abdullah

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  1. Muhammad Aamir Manzoor
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  2. Yan Xu
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  3. Zhengxin lv
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  4. Jieming Xu
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  10. Ruie Liu
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  11. Songtao Jiu
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Contributions

MAM conceived and designed the experiments; YX, ZL, JX, YW, WS, XL, LW, MA, and RL contributed reagents/materials/analysis tools; SJ and CZ provided guidance in the whole manuscript. MAM wrote the article. All authors read and approved the final manuscript.

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Correspondence to Songtao Jiu or Caixi Zhang.

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Communicated by Anis Ali Shah.

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Manzoor, M.A., Xu, Y., lv, Z. et al. Comparative genomics of N-acetyl-5-methoxytryptamine members in four Prunus species with insights into bud dormancy and abiotic stress responses in Prunus avium. Plant Cell Rep 43, 89 (2024). https://doi.org/10.1007/s00299-024-03184-0

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