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TALE-based organellar genome editing and gene expression in plants

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Abstract

Key message

TALE-based editors provide an alternative way to engineer the organellar genomes in plants. We update and discuss the most recent developments of TALE-based organellar genome editing in plants.

Abstract

Gene editing tools have been widely used to modify the nuclear genomes of plants for various basic research and biotechnological applications. The clustered regularly interspaced short palindromic repeats (CRISPR)/Cas9 editing platform is the most commonly used technique because of its ease of use, fast speed, and low cost; however, it encounters difficulty when being delivered to plant organelles for gene editing. In contrast, protein-based editing technologies, such as transcription activator-like effector (TALE)-based tools, could be easily delivered, expressed, and targeted to organelles in plants via Agrobacteria-mediated nuclear transformation. Therefore, TALE-based editors provide an alternative way to engineer the organellar genomes in plants since the conventional chloroplast transformation method encounters technical challenges and is limited to certain species, and the direct transformation of mitochondria in higher plants is not yet possible. In this review, we update and discuss the most recent developments of TALE-based organellar genome editing in plants.

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Funding

This work was financially supported in part by the grant (NSTC 112-2313-B-006-004) to C.-C. Chang from the National Science and Technology Council, Taiwan.

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Authors and Affiliations

  1. Agricultural Biotechnology Research Center, Academia Sinica, Tainan, 71150, Taiwan

    Jer-Young Lin, Yu-Chang Liu & Ming-Tsair Chan

  2. Department of Biotechnology and Bioindustry Sciences, National Cheng Kung University, Tainan, 70101, Taiwan

    Jer-Young Lin, Yan-Hao Tseng, Ming-Tsair Chan & Ching-Chun Chang

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  2. Yu-Chang Liu
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Contributions

Data curation, YCL, JYL, YHT, MTC, and CCC; writing—original draft preparation, CCC; writing—review and editing, JYL, MTC, and CCC; visualization, JYL, YCL and CCC; supervision, MTC and CCC; funding acquisition, CCC. All authors have read and approved the final manuscript.

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Correspondence to Ming-Tsair Chan or Ching-Chun Chang.

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Communicated by Wusheng Liu.

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Lin, JY., Liu, YC., Tseng, YH. et al. TALE-based organellar genome editing and gene expression in plants. Plant Cell Rep 43, 61 (2024). https://doi.org/10.1007/s00299-024-03150-w

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