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Plasma membrane H+-ATPase is involved in methyl jasmonate-induced root hair formation in lettuce (Lactuca sativa L.) seedlings

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Our results show that methyl jasmonate induces plasma membrane H + -ATPase activity and subsequently influences the apoplastic pH of trichoblasts to maintain a cell wall pH environment appropriate for root hair development.

Abstract

Root hairs, which arise from root epidermal cells, are tubular structures that increase the efficiency of water absorption and nutrient uptake. Plant hormones are critical regulators of root hair development. In this study, we investigated the regulatory role of the plasma membrane (PM) H+-ATPase in methyl jasmonate (MeJA)-induced root hair formation. We found that MeJA had a pronounced effect on the promotion of root hair formation in lettuce seedlings, but that this effect was blocked by the PM H+-ATPase inhibitor vanadate. Furthermore, MeJA treatment increased PM H+-ATPase activity in parallel with H+ efflux from the root tips of lettuce seedlings and rhizosphere acidification. Our results also showed that MeJA-induced root hair formation was accompanied by hydrogen peroxide accumulation. The apoplastic acidification acted in concert with reactive oxygen species to modulate root hair formation. Our results suggest that the effect of MeJA on root hair formation is mediated by modulation of PM H+-ATPase activity.

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Acknowledgments

This work was funded by the National Natural Science Foundation of China (grant No. J1210056). We are grateful to Prof. Y. Y. Zhu of Nanjing agricultural university for technical support and to anonymous reviewers for valuable comments and suggestions for revising the manuscript.

Conflict of interest

All authors read and agreed with the final manuscript and have no conflict of interest.

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Authors and Affiliations

  1. College of Life Sciences, Nanjing Agricultural University, Nanjing, 210095, China

    Changhua Zhu, Na Yang, Xiaoling Ma, Guijun Li, Meng Qian, Kai Xia & Lijun Gan

  2. CH BIOTECH R&D CO. LTD., No.121, Xian’an Rd., Xianxi Township, 50741, Changhua, Taiwan, ROC

    Denny Ng

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  1. Changhua Zhu
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  2. Na Yang
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  3. Xiaoling Ma
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Correspondence to Kai Xia or Lijun Gan.

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Zhu, C., Yang, N., Ma, X. et al. Plasma membrane H+-ATPase is involved in methyl jasmonate-induced root hair formation in lettuce (Lactuca sativa L.) seedlings. Plant Cell Rep 34, 1025–1036 (2015). https://doi.org/10.1007/s00299-015-1762-4

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