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Erlotinib-entrapped gellan gum/carboxymethyl fenugreek seed mucilage-based thermo/pH-sensitive matrices

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Abstract

Carboxymethyl fenugreek seed mucilage-grafted poly-N-isopropylacrylamide/montmorillonite [CFSM-g-PNIPA/MMT] nanocomposites (NCs) were dually crosslinked with gellan gum (GG) to afford interpenetrating polymer network (IPN) matrices for effective delivery of erlotinib HCl (ERL) to the triple-negative breast cancer (TNBC) cells. The IPN matrices with different reinforcing clay (MMT) contents (0–20%) demonstrated comparable size (1.64–1.80 mm) and acceptable drug loading capacity (DEE, 47.97–89.35%). The infrared, thermal and X-ray analyses implied the compatibility between drug and matrix constituents, and the SEM studies conferred the spherical morphology of the IPN matrices. Moreover, these hydrogel matrices portrayed temperature-responsive swelling profiles and their molar masses between crosslinks \(\left( {\bar{M}_{{\text{c}}} } \right)\) were increased with temperature. Among different matrices, the composites containing 20% MMT (F-3) revealed the sustained drug release profiles, which were best fitted to the Higuchi model with an anomalous transport-driven mechanism. These matrices (F-3) also exhibited pH-dependent swelling and drug release patterns. Furthermore, these matrices evidenced a slower biodegradability as compared to the reference composites (F-1,0% MMT). The mucin adsorption ability of matrices followed the Freundlich isotherm. The matrices (F-3) also displayed enhanced anti-proliferative and apoptosis-inducing potentials on TNBC cells relative to pure ERL. Thus, the GG/CFSM-based IPN matrices could be employed as efficient drug delivery vehicles for breast cancer therapy.

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Funding

The present work was supported by the fellowship of China Postdoctoral Science Foundation (grant no. 2021MD703857; grant recipient, Dr. Hriday Bera).

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Author notes

  1. Yasir Faraz Abbasi and Hriday Bera have equally contributed to this work.

Authors and Affiliations

  1. Wuya College of Innovation, Shenyang Pharmaceutical University, Liaoning, Shenyang, 110013, China

    Yasir Faraz Abbasi & Hriday Bera

  2. Dr. B. C. Roy College of Pharmacy and Allied Health Sciences, Durgapur, 713206, India

    Hriday Bera

  3. Pritzker School of Molecular Engineering Ben May Department for Cancer Research, University of Chicago, Chicago, IL, USA

    Abhimanyu Thakur

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  1. Yasir Faraz Abbasi
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  2. Hriday Bera
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  3. Abhimanyu Thakur
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Contributions

YFA contributed to preparation and characterization of drug-loaded formulations, writing, reviewing and editing; HB contributed to conceptualization, characterization of drug-loaded formulations, supervision, writing, reviewing and editing; AT involved in cell culture experiments of various scaffolds; YFA and HB contributed equally.

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Correspondence to Hriday Bera.

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Abbasi, Y.F., Bera, H. & Thakur, A. Erlotinib-entrapped gellan gum/carboxymethyl fenugreek seed mucilage-based thermo/pH-sensitive matrices. Polym. Bull. (2024). https://doi.org/10.1007/s00289-024-05240-x

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