Skip to main content

Advertisement

SpringerLink
Log in

In-situ production of silver nanobiocomposite using surface layer protein of Lactobacillus helveticus and aqueous extract of dried Juglans regia green husk and investigation of antibacterial activity

  • Original Paper
  • Published:
Polymer Bulletin Aims and scope Submit manuscript

Abstract

One way to overcome the challenge of the active surface of nanoparticles and their tendency to aggregate is to use a biopolymer. The surface layer protein (S-layer) is a good candidate for trapping nanoparticles. S-layer molecules bridge the space between neighbor nanoparticles and prevent them from aggregating. In this study, S-layer as a renewable biopolymer was extracted from Lactobacillus helveticus, nanobiocomposite prepared by Intermatrix synthesis (IMS) method and S-layer used as a matrix for silver nanoparticles(AgNPs). The 3D structure of Lactobacillus helveticus S-layer was predicted with I-TASSER server, and then the binding of silver ions to this S-layer was confirmed by Molegro Virtual Docker software. After precursor ions binding to S-layer, the aqueous extract of dried Juglans regia green husk was added as a nanoparticle producing agent. Nanobiocomposite analysis with UV–Vis spectroscopy showed that the process was completed after 23 days. Then with the Field Emission Scanning Electron Microscope (FESEM), the distribution of nanoparticles on the S-layer matrix was observed. With the Energy-dispersive X-ray (EDX), the presence of AgNPs was confirmed. The antimicrobial effect of the nanobiocomposite against Pseudomonas aeruginosa PAO1 was investigated by the agar well diffusion method. There are three achievements in this study: (1) AgNPs were stabilized, (2) the antibacterial bionanocomposte was synthesized, and (3) renewable biopolymer (S-layer) as matrix and the aqueous extract of dried Juglans regia green husk as producing agent of AgNPs, introduce an ecofriendly method for fabrication of bionanocomposite.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6
Fig. 7
Fig. 8
Fig. 9

Similar content being viewed by others

References

  1. Ijaz M, Zafar M, Iqbal T (2020) Green synthesis of silver nanoparticles by using various extracts: a review. Inorg Nano-Metal Chem 51:744–755

    Article  Google Scholar 

  2. Salayova A, Bedlovicova Z, Daneu N, Balaz M, Lukacova Bujnakova Z, Balazova L, Tkacikova L (2021) Green synthesis of silver nanoparticles with antibacterial activity using various medicinal plant extracts: morphology and antibacterial efficacy. Nanomaterials 11:1–20

    Article  Google Scholar 

  3. Bastos-Arrieta J, Muñoz J, Stenbock-Fermor A, Muñoz M, Muraviev DN, Céspedes F, Baeza M (2016) Intermatrix Synthesis as a rapid, inexpensive and reproducible methodology for the in situ functionalization of nanostructured surfaces with quantum dots. Appl Surf Sci 368:417–426

    Article  CAS  Google Scholar 

  4. Zahran M, Marei AH (2019) Innovative natural polymer metal nanocomposites and their antimicrobial activity. Int J Biol Macromol 136:586–596

    Article  CAS  PubMed  Google Scholar 

  5. Huggias S, Bolla PA, Azcarate JC, Serradell MA, Casella ML, Peruzzo PJ (2021) Noble metal nanoparticles-based heterogeneous bionano-catalysts supported on S-layer protein/polyurethane system. Catal Today 372:98–106

    Article  CAS  Google Scholar 

  6. Pum D, Breitwieser A, Sleytr UB (2021) Patterns in Nature—S-Layer Lattices of Bacterial and Archaeal Cells. Curr Comput-Aided Drug Des 11:1–17

    Google Scholar 

  7. Boot HJ, Kolen CP, Van Noort JM, Pouwels PH (1993) S-layer protein of Lactobacillus acidophilus ATCC 4356: purification, expression in Escherichia coli, and nucleotide sequence of the corresponding gene. J Bacteriol 175:6089–6096

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  8. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685

    Article  CAS  PubMed  Google Scholar 

  9. Selenska-Pobel S, Reitz T, Schöneman R, Herrmansdörfer T, Merroun M, Geißler A, Bartolome J, Bartolome F, Miguel Garcia L, Wilhelm F, Rogalev A (2011) Magnetic Au nanoparticles on archaeal S-layer ghosts as templates. Nanomater Nanotechnol 1:8–16

    Article  Google Scholar 

  10. Scimeca M, Bischetti S, Lamsira HK, Bonfiglio R, Bonanno E (2018) Energy Dispersive X-ray (EDX) microanalysis: A powerful tool in biomedical research and diagnosis. Eur J Histochem 62:1–10

    CAS  Google Scholar 

  11. Balouiri M, Sadiki M, Ibnsouda SK (2016) Methods for in vitro evaluating antimicrobial activity: A review. J Pharm Anal 6:71–79

    Article  PubMed  Google Scholar 

  12. Sharma D, Kanchi S, Bisetty K (2019) Biogenic synthesis of nanoparticles: A review. J Chem 12:3576–3600

    CAS  Google Scholar 

  13. Domènech B, Muñoz M, Muraviev DN, Macanás J (2013) Polymer-silver nanocomposites as antibacterial materials.In: Microbial pathogens and strategies for combating them: science, technology and education, Formatex Research Center, Badjoz, pp 630–640.

  14. Palza H (2015) Antimicrobial polymers with metal nanoparticles. J Mol Sci 16:2099–2116

    Article  CAS  Google Scholar 

  15. Guo Q, Ghadiri R, Weigel T, Aumann A, Gurevich EL, Esen C, Ostendorf A (2014) Comparison of in situ and ex situ methods for synthesis of two-photon polymerization polymer nanocomposites. Polymers 6:2037–2050

    Article  Google Scholar 

  16. Grossman RF, Nwabunma D (2013) Biopolymer nanocomposite. Wiley, The United States of America

    Google Scholar 

  17. Merroun M, Rossberg A, Hennig C, Scheinost AC, Selenska-Pobell S (2007) Spectroscopic characterization of gold nanoparticles formed by cells and S-layer protein of Bacillus sphaericus JG-A12. Mater Sci Eng C 27:188–192

    Article  CAS  Google Scholar 

  18. Pum D, Toca-herrera JL, Sleytr UB (2013) S-layer protein self-assembly. Int J Mol Sci 14(2):2484–2501

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  19. Kasaragadda S, Alarifi IM, Rahimi-Gorji M, Asmatulu R (2020) Investigating the effects of surface superhydrophobicity on moisture ingression of nanofiber-reinforced bio-composite structures. Microsyst Technol 26:447–459

    Article  CAS  Google Scholar 

  20. Qamsari EM, Kermanshahi RK, Erfan M, Ghadam P, Sardari S, Eslami N (2017) Characteristics of surface layer proteins from two new and native strains of Lactobacillus brevis. Int J Biol Macromol 95:1004–1010

    Article  Google Scholar 

  21. Chinni G, Alarifi IM, Rahimi-Gorji M, Asmatulu R (2019) Investigating the effects of process parameters on microalgae growth, lipid extraction, and stable nanoemulsion productions. J Mol Liq 291:1–7

    Article  Google Scholar 

  22. Mei L, Lu Z, Zhang X, Li C, Jia Y (2014) Polymer-Ag nanocomposites with enhanced antimicrobial activity against bacterial infection. Appl Mater Interfaces 6:15813–15821

    Article  CAS  Google Scholar 

  23. Zheng Z, Huang Q, Guan H, Liu S (2015) In situ synthesis of silver nanoparticles dispersed or wrapped by a Cordyceps sinensis exopolysaccharide in water and their catalytic activity. RSC Adv 5:69790–69799

    Article  CAS  Google Scholar 

  24. Krajczewski J, Kołątaj K, Kudelski A (2017) Plasmonic nanoparticles in chemical analysis. RSC Adv 7:17559–17576

    Article  CAS  Google Scholar 

  25. Asmatulu R, Erukala KS, Shinde M, Alarifi IM, Rahimi Gorji M (2019) Investigating the effects of surface treatments on adhesion properties of protective microsyss on carbon fiber-reinforced composite laminates. Surf Coatings Technol 380:1–11

    Article  Google Scholar 

  26. Rajkumari J, Magdalane CM, Siddhardha B, Madhavan J, Ramalingam G, Al-Dhabi NA, Kaviyarasu K (2019) Synthesis of titanium oxide nanoparticles using Aloe barbadensis mill and evaluation of its antibiofilm potential against Pseudomonas aeruginosa PAO1. J Photochem Photobiol B Biol 201:111667

    Article  CAS  Google Scholar 

  27. Abbasi Z, Feizi S, Taghipour E, Ghadam P (2017) Green synthesis of silver nanoparticles using aqueous extract of dried Juglans regia green husk and examination of its biological properties. Green Process Synth 6:477–485

    CAS  Google Scholar 

  28. Prado-Acosta M, Ruzal SM, Allievi MC, Palomino MM, Rivas CS (2010) Synergistic effects of the Lactobacillus acidophilus surface layer and nisin on bacterial growth. Appl Environ Microbiol 76:974–977

    Article  CAS  PubMed  Google Scholar 

  29. Wahl R, Mertig M, Raff J, Selenska-Pobell S, Pompe W (2001) Electron-beam induced formation of highly ordered palladium and platinum nanoparticle arrays on the S layer of Bacillus sphaericusNCTC 9602. Adv Mater 13:736–740

    Article  CAS  Google Scholar 

  30. Dieluweit S, Pum D, Sleytr UB, Kautek W (2005) Monodisperse gold nanoparticles formed on bacterial crystalline surface layers (S-layers) by electroless deposition. Mater Sci Eng C 25:727–732

    Article  Google Scholar 

  31. Mark SS, Bergkvist M, Bhatnagar P, Welch C, Goodyear AL, Yang X, Angert ER, Batt CA (2007) Thin film processing using S-layer proteins: Biotemplated assembly of colloidal gold etch masks for fabrication of silicon nanopillar arrays. Colloids Surf 57:161–173

    Article  CAS  Google Scholar 

  32. Puranik SS, Joshi HM, Ogale SB, Paknikar KM (2008) Hydrazine based facile synthesis and ordered assembly of metal nanoparticles (Au, Ag) on a bacterial surface layer protein template. J Nanosci Nanotechnol 8:3565–3569

    Article  CAS  PubMed  Google Scholar 

  33. Wahl R, Engelhardt H, Pompe W, Mertig M (2005) Multivariate statistical analysis of two-dimensional metal cluster arrays grown in vitro on a bacterial surface layer. Chem Mater 17:1887–1894

    Article  CAS  Google Scholar 

  34. Mertig M, Wahl R, Lehmann M, Simon P, Pompe W (2001) Formation and manipulation of regular metallic nanoparticle arrays on bacterial surface layers: an advanced TEM study. Eur Phys J 16:317–320

    CAS  Google Scholar 

  35. Shenton W, Pum D, Sleytr UB, Mann S (1997) Synthesis of cadmiumsulphide superlattices using self-assembled bacterial S-layers. Nat 389:585–587

    Article  CAS  Google Scholar 

  36. Bolla PA, Sanz A, Huggias S, Ruggera JF, Serradell MA, Casella ML (2020) Regular arrangement of Pt nanoparticles on S-layer proteins isolated from Lactobacillus kefiri: synthesis and catalytic application. Mol Catal 481:1–9

    Google Scholar 

Download references

Acknowledgements

This work has been supported by Alzahra University, Tehran, Iran.

Author information

Authors and Affiliations

  1. Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran

    Fatemeh Rahimzadeh, Parinaz Ghadam & Mahboobeh Zarrabi

  2. Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran

    Rouha Kasra-Kermanshahi

Authors
  1. Fatemeh Rahimzadeh
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Parinaz Ghadam
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Rouha Kasra-Kermanshahi
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Mahboobeh Zarrabi
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Parinaz Ghadam.

Ethics declarations

Conflict of interest

The authors declare that there is no conflict of interests regarding the publication of this paper.

Additional information

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Rahimzadeh, F., Ghadam, P., Kasra-Kermanshahi, R. et al. In-situ production of silver nanobiocomposite using surface layer protein of Lactobacillus helveticus and aqueous extract of dried Juglans regia green husk and investigation of antibacterial activity. Polym. Bull. 79, 8353–8367 (2022). https://doi.org/10.1007/s00289-021-03895-4

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s00289-021-03895-4

Keywords

Search

Navigation