Abstract
Protein-imprinted polyacrylamide (PAM)-grafted calcium alginate (CaA) microspheres were synthesized via free-radical grafting polymerization with potassium persulfate and sodium bisulfite as initiators. FTIR, 13C-NMR and SEM were used for characterization. Swelling behaviors were studied and it was found that modified calcium alginate (CaA) microspheres possessed larger swelling degree than calcium alginate, because grafted hydrophilic PAM side chains contributed to more water absorption. Moreover, the durability of the swollen samples was improved for the modified microspheres. Compared with calcium alginate microspheres, modified calcium alginate samples exhibited higher absorption capacity (approximately, 13.1 and 10.3 mg/g) for MIPs in single and binary protein solution. The rebinding selectivity of CaA-g-PAM microspheres (approximately, 2.7) was higher than that of calcium alginate (CaA) microspheres (approximately, 1.5) in binary protein solution. It was suggested that the introduction of PAM side chains was beneficial for the specific recognition of protein.
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References
Okutucu B, Onal S (2011) Molecularly imprinted polymers for separation of various sugars from human urine. Talanta 87:74–79
Hande PE, Samui AB, Kulkarni PS (2015) Highly selective monitoring of metals by using ion-imprinted polymers. Sci Pollut R 22:7375–7404
He J, Chen JP (2014) Cu(II)-imprinted poly(vinyl alcohol)/poly(acrylic acid) membrane for greater enhancement in sequestration of copper ion in the presence of competitive heavy metal ions: material development, process demonstration, and study of mechanisms. Ind Eng Chem Res 53:20223–20233
Liu X, Fang H, Yu L (2013) Molecularly imprinted photonic polymer based on β-cyclodextrin for amino acid sensing. Talanta 116:283–289
Mirmohseni A, Shojaei M, Pourata R (2014) Experimental design and multi-objective optimization of molecularly imprinted polymers for monosaccharides. RSC Adv 4:20177–20184
Kyzas GZ, Bikiaris DN, Lazaridis NK (2009) Selective separation of basic and reactive dyes by molecularly imprinted polymers (MIPs). Chem Eng J 149:263–272
Yang C, Yan X, Guo H, Fu G (2016) Synthesis of surface protein-imprinted nanoparticles endowed with reversible physical cross-links. Biosens Bioelectron 75:129–135
Li L, Lu Y, Bie Z, Chen H, Liu Z (2013) Photolithographic boronate affinity molecular imprinting: a general and facile approach for glycoprotein imprinting. Angew Chem Int Ed 52:7451–7454
Kryscio DR, Peppas NA (2012) Critical review and perspective of macromolecularly imprinted polymers. Acta Biomater 8:461–473
Verheyen E, Schillemans JP, van Wijk M, Demeniex MA, Hennink WE, van Nostrum CF (2011) Challenges for the effective molecular imprinting of proteins. Biomaterials 32:3008–3020
Whitcombe MJ, Chianella I, Larcombe L, Piletsky SA, Noble J, Porter R, Horgan A (2011) The rational development of molecularly imprinted polymer-based sensors for protein detection. Chem Soc Rev 40:1547–1571
Liu J, Ying X, Wang H, Li X, Zhang W (2016) BSA imprinted polyethylene glycol grafted calcium alginate hydrogel microspheres. J Appl Polym Sci 133:43617
Reddy SM, Hawkins DM, Phan QT, Stevenson D, Warriner K (2013) Protein detection using hydrogel-based molecularly imprinted polymers integrated with dual polarisation interferometry. Sens Actuators B Chem 176:190–197
Zayats M, Kanwar M, Ostermeier M, Searson PC (2011) Molecular imprinting of maltose binding protein: tuning protein recognition at the molecular level. Macromolecules 44:3966–3972
Lee KY, Mooney DJ (2012) Alginate: properties and biomedical applications. Prog Polym Sci 37:106–126
Abdulla R, Ravindra P (2013) Characterization of cross linked Burkholderia cepacia lipase in alginate and κ-carrageenan hybrid matrix. J Taiwan Inst Chem Eng 44:545–551
Bayer CL, Peppas NA (2008) Advances in recognitive, conductive and responsive delivery systems. J Controll Release 132:216–221
Kryscio DR, Peppas NA (2009) Mimicking biological delivery through feedback-controlled drug release systems based on molecular imprinting. AIChE J 55:1311–1324
Zhang F, Cheng G, Ying X (2006) Emulsion and macromolecules templated alginate based polymer microspheres. React Funct Polym 66:712–719
Zhao K, Huang J, Ying X, Cheng G (2008) Macromolecularly imprinted calcium phosphate/alginate hybrid polymer microspheres with the surface imprinting of bovine serum albumin in inverse-phase suspension. J Appl Polym Sci 109:2687–2693
Zhao K, Cheng G, Huang J, Ying X (2008) Rebinding and recognition properties of protein-macromolecularly imprinted calcium phosphate/alginate hybrid polymer microspheres. React Funct Polym 68:732–741
Zhao K, Wei J, Cheng G, Yang C, Chen L (2009) Preparation of bovine serum albumin-imprinted calcium polyacrylate/alginate hybrid microspheres via Ca2+ crosslinking. J Appl Polym Sci 113:1133–1140
Herrero EP, Del Valle EMM, Peppas NA (2010) Protein imprinting by means of alginate-based polymer microcapsules. Ind Eng Chem Res 49:9811–9814
Li L, Ying X, Liu J, Li X, Zhang W (2015) Protein-imprinted polyurethane-grafted calcium alginate hydrogel microspheres. J Appl Polym Sci 132:42140
Ying X, Qi L, Li X, Zhang W, Cheng G (2013) Stimuli-responsive recognition of BSA-imprinted poly vinyl acetate grafted calcium alginate core-shell hydrogel microspheres. J Appl Polym Sci 127:3898–3909
Li L, Ying X, Liu J, Li X, Zhang W (2015) Molecularly imprinted polyurethane grafted calcium alginate hydrogel with specific recognition for proteins. Mater Lett 143:248–251
Bayer CL, Herrero EP, Peppas NA (2011) Alginate films as macromolecular imprinted matrices. J Biomater Sci Polym Ed 22:1523–1534
Wang J, Ying X, Li X, Zhang W (2014) Preparation, characterization and swelling behaviors of polyurethane-grafted calcium alginate hydrogels. Mater Lett 126:263–266
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The authors thank the support of the 2017 College Joint Funded Project of Natural Science Foundation of Fujian Province (2017J01416), the China Scholarship Council, Fuzhou University Science Technology Development Fund (2014-XQ-23) and Fuzhou University Postdoctoral Station Fund (XDJ201206).
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Ying, X., Wang, H., Liu, J. et al. Polyacrylamide-grafted calcium alginate microspheres as protein-imprinting materials. Polym. Bull. 75, 2139–2150 (2018). https://doi.org/10.1007/s00289-017-2138-4
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DOI: https://doi.org/10.1007/s00289-017-2138-4