Abstract
Screening probiotics are crucial for assessing their safety, security, and further production of functional foods for human health. The present study aimed to isolate and identify bacteria from raw cow’s milk samples that exhibit health benefits upon consumption. We characterized the probiotic properties of Lactobacillus plantarum (also called Lactiplantibacillus plantarum) strains CMGC2 and CMJC7 isolated from cow milk by in vitro study. The strains exhibited tolerance to simulated gastric conditions and were further identified by 16S rRNA sequencing as Lactobacillus plantarum (L. plantarum) CMGC2 and CMJC7. Both isolates were evaluated in vitro for their probiotic attributes, viz. hydrophobicity, autoaggregation, co-aggregation, lysozyme tolerance, antibacterial activity, antibiotic susceptibility, hemolytic activity, and phenol tolerance. The isolates CMGC2 and CMJC7 showed excellent probiotic attributes; hence, both strains were selected to produce coconut and carrot juice mixed beverages (CCMB). The CCMB was evaluated for the pH, acid-production rate, and total viable bacterial counts. The results showed that the CCMB was an excellent medium for the growth of CMGC2 and CMJC7 as it supported adequate growth of organisms (8.93 CFU/mL and 8.68 CFU/mL, respectively) even after 48 h of incubation. In conclusion, CMGC2 and CMJC7 can be used to develop different beverages possessing nutritive and probiotic values, and these beverages can be used for producing unique products.
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Data Availability
The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequence strain CMGC2 and CMJC7 are MT928890 and MT973993.
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Acknowledgements
The authors acknowledge Christ College, Rajkot, India, for supporting the research. The cow herder of Amreli, who helped collect milk samples. Dr. Subarna Thakur, Department of Bioinformatics, University of North Bengal, helped to generate a phylogenetic tree.
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DPS contributed to study conception and design, acquisition of data, analysis, interpretation of data, and drafting of the manuscript; JJG contributed to analysis and interpretation of data and critical revision. Both authors have approved the final version for submission.
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Senjaliya, D.P., Georrge, J.J. Functional Characterization of Lactobacillus plantarum Isolated from Cow Milk and the Development of Fermented Coconut and Carrot Juice Mixed Beverage. Curr Microbiol 80, 139 (2023). https://doi.org/10.1007/s00284-023-03258-4
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DOI: https://doi.org/10.1007/s00284-023-03258-4