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Biomass-Degrading Enzyme(s) Production and Biomass Degradation by a Novel Streptomyces thermocarboxydus

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Abstract

Modern society has a great challenge to decrease waste and minimize the adverse effects of wastes on the economy, environment, and individual health. Thus, this study focuses on the use of eight agro-wastes (banana peel, barley straw, canola straw, pomegranate peel, orange peel, pumpkin pulp+seeds, maple leaf, and brewer’s spent grains) by a novel bacterium (Streptomyces thermocarboxydus) for enzymes production. Further, the study explored the subsequent degradation of those wastes by the bacterium. This bacterium was isolated from forest soil and identified as Streptomyces thermocarboxydus by 16S rRNA sequence analysis. The biodegrading capability of S. thermocarboxydus was determined by observing the clear zone around the colony cultured on the agar plate containing the different biomasses as sole carbon sources and calculating the substrate degradation ratios. Furthermore, scanning electron microscopy images of eight agro-wastes before and after bacterial treatment and weight loss of agro-wastes revealed the bacterium degraded the biomasses. The different trends of enzyme activities were observed for various wastes, and the maximum activity depended on the type of agro-wastes. Overall, S. thermocarboxydus was found to be a potential candidate for pectinase and xylanase production. The enzyme production varies with the concentration of the biomasses.

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References

  1. World Population Growth Rate 1950–2022 (2022) https://www.un.org/development/desa/pd/. Accessed 5 Aug 2022

  2. Yunis J, Aliakbari E (2021) Generation and management of municipal solid waste: how’s canada doing? Fraser Insitute, Canada

    Google Scholar 

  3. Mihajlovski K, Buntić A, Milić M et al (2020) From agricultural waste to biofuel: enzymatic potential of a bacterial isolate Streptomyces fulvissimus CKS7 for bioethanol production. Waste Biomass Valoriz. https://doi.org/10.1007/s12649-020-00960-3

    Article  Google Scholar 

  4. Panda SK, Mishra SS, Kayitesi E, Ray RC (2016) Microbial-processing of fruit and vegetable wastes for production of vital enzymes and organic acids: biotechnology and scopes. Environ Res 146:161–172. https://doi.org/10.1016/j.envres.2015.12.035

    Article  CAS  Google Scholar 

  5. Shrestha S, Mokale AL, Zhang J, Qin W (2020) Different facets of lignocellulosic biomass including pectin and its perspectives. Waste Biomass Valoriz. https://doi.org/10.1007/s12649-020-01305-w

    Article  Google Scholar 

  6. Shrestha S, Rahman S, Qin W (2021) New insights in pectinase production development and industrial applications. Appl Microbiol Biotechnol 105:9069–9087. https://doi.org/10.1007/s00253-021-11705-0

    Article  CAS  Google Scholar 

  7. Ravindran R, Hassan SS, Williams GA, Jaiswal AK (2018) A review on bioconversion of agro-industrial wastes to industrially important enzymes. Bioengineering 5:1–20. https://doi.org/10.3390/bioengineering5040093

    Article  CAS  Google Scholar 

  8. Mussatto SI, Ballesteros LF, Martins S, Teixeira JA (2012) Use of Agro-Industrial Wastes in Solid-State Fermentation Processes. Industrial waste; Kuan-yeow Show (Ed.) Croatia, ISBN: 978–953- 51–0253–3, InTech

  9. Singh K, Kumar T, Prince et al (2019) A review on conversion of food wastes and by-products into value added products. Int J Chem Stud 7:2068–2073

    CAS  Google Scholar 

  10. Ning P, Yang G, Hu L et al (2021) Recent advances in the valorization of plant biomass. Biotechnol Biofuels 14:1–22. https://doi.org/10.1186/s13068-021-01949-3

    Article  Google Scholar 

  11. Shrestha S, Khatiwada JR, Zhang X et al (2021) Screening and molecular identification of novel pectinolytic bacteria from forest soil. Fermentation 7:12. https://doi.org/10.3390/fermentation7010040

    Article  CAS  Google Scholar 

  12. Oumer OJ (2017) Pectinase: substrate, production and their biotechnological applications. Int J Environ Agric Biotechnol. 2:1007–1014. https://doi.org/10.22161/ijeab/2.3.1

    Article  Google Scholar 

  13. Bharathiraja S, Suriya J, Krishnan M et al (2017) Production of Enzymes From Agricultural Wastes and Their Potential Industrial Applications, 1st edn. Elsevier, Armsterdam

    Google Scholar 

  14. Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428. https://doi.org/10.1021/ac60147a030

    Article  CAS  Google Scholar 

  15. Chen F, Ye J, Chio C et al (2020) A simplified quick microbial genomic DNA extraction via freeze–thawing cycles. Mol Biol Rep 47:703–709. https://doi.org/10.1007/s11033-019-05176-w

    Article  CAS  Google Scholar 

  16. National Center for BIotechnology Information (2022) https://blast.ncbi.nlm.nih.gov/Blast.cgi?. Accessed 6 Aug 2022.

  17. Kumar S, Stecher G, Tamura K (2016) MEGA7: Molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33:1870–1874. https://doi.org/10.1093/molbev/msw054

    Article  CAS  Google Scholar 

  18. Guo H, Chen H, Fan L et al (2017) Enzymes produced by biomass-degrading bacteria can efficiently hydrolyze algal cell walls and facilitate lipid extraction. Renew Energy 109:195–201. https://doi.org/10.1016/j.renene.2017.03.025

    Article  CAS  Google Scholar 

  19. Wu Y, Guo H, Rahman MS et al (2021) Biological pretreatment of corn stover for enhancing enzymatic hydrolysis using Bacillus sp. P3. Bioresour Bioprocess 8:1. https://doi.org/10.1186/s40643-021-00445-8

    Article  Google Scholar 

  20. Kasana RC, Salwan R, Dhar H et al (2008) A rapid and easy method for the detection of microbial cellulases on agar plates using Gram’s iodine. Curr Microbiol 57:503–507. https://doi.org/10.1007/s00284-008-9276-8

    Article  CAS  Google Scholar 

  21. Kim SB, Falconer C, Williams E, Goodfellow M (1998) Two moderately thermophilic carboxydotrophic species from soil. Int J Syst Bacteriol 59:68

    Google Scholar 

  22. Thite VS, Nerurkar AS (2018) Physicochemical characterization of pectinase activity from Bacillus spp. and their accessory role in synergism with crude xylanase and commercial cellulase in enzyme cocktail mediated saccharification of agrowaste biomass. J Appl Microbiol 124:1147–1163. https://doi.org/10.1111/jam.13718

    Article  CAS  Google Scholar 

  23. Ramírez-Tapias YA, Rivero CW, Britos CN, Trelles JA (2015) Alkaline and thermostable polygalacturonase from Streptomyces halstedii ATCC 10897 with applications in waste waters. Biocatal Agric Biotechnol 4:221–228. https://doi.org/10.1016/j.bcab.2014.12.004

    Article  Google Scholar 

  24. de Lima Procópio RE, da Silva IR, Martins MK et al (2012) Antibiotics produced by streptomyces. Brazilian J Infect Dis 16:466–471. https://doi.org/10.1016/j.bjid.2012.08.014

    Article  Google Scholar 

  25. Beg Q, Bhushan B, Kapoor M, Hoondal G (2000) Production and characterization of thermostable xylanase and pectinase from Streptomyces sp. QG-11-3. J Ind Microbiol Biotechnol 24:396–402. https://doi.org/10.1038/sj.jim.7000010

    Article  CAS  Google Scholar 

  26. Ghazala I, Sayari N, Ben RM et al (2015) Assessment of pectinase production by Bacillus mojavensis I4 using an economical substrate and its potential application in oil sesame extraction. J Food Sci Technol 52:7710–7722. https://doi.org/10.1007/s13197-015-1964-3

    Article  CAS  Google Scholar 

  27. Banerjee G, Car S, Scott-Craig JS et al (2011) Alkaline peroxide pretreatment of corn stover: effects of biomass, peroxide, and enzyme loading and composition on yields of glucose and xylose. Biotechnol Biofuels 4:1–15. https://doi.org/10.1186/1754-6834-4-16

    Article  CAS  Google Scholar 

  28. Laothanachareon T, Bunterngsook B, Champreda V (2022) Profiling multi-enzyme activities of Aspergillus niger strains growing on various agro-industrial residues. 3 Biotech 12(1):16. https://doi.org/10.1007/s13205-021-03086-y

    Article  Google Scholar 

  29. Sharma D, Nagpal R, Agrawal S et al (2021) Eco-friendly bleaching of agrowaste wheat straw using crude alkalo-thermotolerant cellulase-free xylano-pectinolytic enzymes. Appl Biochem Biotechnol. https://doi.org/10.1007/s12010-021-03641-6

    Article  Google Scholar 

  30. Singh A, Varghese LM, Battan B et al (2020) Eco-friendly scouring of ramie fibers using crude xylano-pectinolytic enzymes for textile purpose. Environ Sci Pollut Res 27:6701–6710. https://doi.org/10.1007/s11356-019-07424-9

    Article  CAS  Google Scholar 

  31. Limaye L, Patil R, Ranadive P, Kamath G (2017) Application of potent actinomycete strains for bio-degradation of domestic agro-waste by composting and treatment of pulp-paper mill effluent. Adv Microbiol 07:94–108. https://doi.org/10.4236/aim.2017.71008

    Article  CAS  Google Scholar 

  32. Saha BC, Qureshi N, Kennedy GJ, Cotta MA (2016) Biological pretreatment of corn stover with white-rot fungus for improved enzymatic hydrolysis. Int Biodeterior Biodegrad 109:29–35. https://doi.org/10.1016/j.ibiod.2015.12.020

    Article  CAS  Google Scholar 

  33. Maki ML, Idrees A, Leung KT, Qin W (2012) Newly isolated and characterized bacteria with great application potential for decomposition of lignocellulosic biomass. J Mol Microbiol Biotechnol 22:156–166. https://doi.org/10.1159/000341107

    Article  CAS  Google Scholar 

  34. Kocabaş SD, Güder S, Özben N (2015) Purification strategies and properties of a low-molecular weight xylanase and its application in agricultural waste biomass hydrolysis. J Mol Catal B Enzym 115:66–75. https://doi.org/10.1016/j.molcatb.2015.01.012

    Article  CAS  Google Scholar 

  35. Poondla V, Bandikari R, Subramanyam R, Reddy Obulam VS (2015) Low temperature active pectinases production by Saccharomyces cerevisiae isolate and their characterization. Biocatal Agric Biotechnol 4:70–76. https://doi.org/10.1016/j.bcab.2014.09.008

    Article  Google Scholar 

  36. Liu X, Jiang Y, Yang S et al (2017) Effects of pectinase treatment on pulping properties and the morphology and structure of bagasse fiber. BioResources 12:7731–7743. https://doi.org/10.15376/biores.12.4.7731-7743

    Article  CAS  Google Scholar 

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Acknowledgements

The authors would like to acknowledge all the individuals who directly or indirectly helped to complete this research.

Funding

This work was supported by the Natural Sciences and Engineering Research Council of Canada (RGPIN-2017–05366) to Wensheng Qin and the Ontario Graduate Scholarship to Sarita Shrestha.

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Authors and Affiliations

  1. Department of Biology, Lakehead University, 955 Oliver Road, Thunder Bay, ON, P7B 5E1, Canada

    Sarita Shrestha, Janak R. Khatiwada, Aristide L. M. Kognou, Chonlong Chio & Wensheng Qin

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  1. Sarita Shrestha
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  2. Janak R. Khatiwada
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  3. Aristide L. M. Kognou
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  4. Chonlong Chio
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  5. Wensheng Qin
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Contributions

The manuscript was designed and written by SS. JRK: helped in molecular analysis. WQ: supervised the study, commented on and helped revise the manuscript. All authors read and approved the final manuscript.

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Correspondence to Wensheng Qin.

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Shrestha, S., Khatiwada, J.R., Kognou, A.L.M. et al. Biomass-Degrading Enzyme(s) Production and Biomass Degradation by a Novel Streptomyces thermocarboxydus. Curr Microbiol 80, 71 (2023). https://doi.org/10.1007/s00284-022-03174-z

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