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Genomic Analysis and Molecular Characteristics in Carbapenem-Resistant Klebsiella pneumoniae Strains

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Abstract

Klebsiella pneumoniae is an important bacterium and responsible for both infections acquired in hospital and community because of its multidrug resistance and the virulence. The aim of this research was to investigate clonal lineages, antibiotic resistance profiles, and virulence factors of the hospital isolated carbapenem-resistant strains. Fifty carbapenem-resistant isolates were phenotypically confirmed extended-spectrum beta-lactamases ESBLs producers. MLST analysis revealed 94% sequence type 11. These isolates mainly belonged to three clones according to the PFGE DNA patterns. PFGE patterns have good discrimination than ST profiles. One isolate, K. pneumoniae KPX, undergoing whole-genome sequencing comprised one circular chromosome and four circular plasmids. This isolate harbored a variety of antimicrobial resistance and virulence determinants. The closest relative of K. pneumoniae KPX was another ST11 clinical isolate recovered Sichuan. In addition, KPC-2 (98.0%), SHV-11 (98.0%), TEM-1 (76.0%), CTX-M (76.0%), oqxB1(66%), qnrS (70%), Int1 (42.0%), sul1 (82.0%), sul2 (96.0%), iutA (88%), iucC(10%), and rmpA2 (100%) genes were presented in multiple drug-resistant isolates. The dataset presented in this study provided the genomic and epidemiological analysis of carbapenem-resistant K. pneumoniae in hospital settings. Antimicrobial-resistant profiles suggested the presence of significant selective antibiotic pressure. Appropriate surveillance is essential to the development of effective control strategies in the prevention of nosocomial infection.

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Acknowledgements

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Funding

The work was supported by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) and Top-notch Academic Programs Project of Jiangsu Higher Education Institution (TAPP, PPZY2015A067). The funders had no role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Author notes

  1. Suying Zhao and Xingyi Qiu have equally contribution to this work. They are co-first authors on this work.

Authors and Affiliations

  1. Department of Laboratory Medicine, Jiangsu Province Hospital of Chinese Medicine, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing, 210029, China

    Suying Zhao, Min Xu, Huiling Cao & Min Li

  2. The Key Laboratory of Modern Toxicology of Ministry of Education and Department of Health Inspection and Quarantine, School of Public Health, Nanjing Medical University, Nanjing, 211166, China

    Xingyi Qiu, Yiting Tan, Xiaotong Xu, Yulai Ge, Yuxin Lin, Zhan Zhang, Lei Li & Qian Wu

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  1. Suying Zhao
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Contributions

QW conceived and designed the experiments and wrote the paper. XYQ, YTT, XTX, YLG, MX, HLC, ML, and YXL performed the experiments. SYZ, QW, ZZ, and LL analyzed the data. SYZ and LL contributed reagents/materials/analysis tools. All the authors read and approved the final manuscript.

Corresponding author

Correspondence to Qian Wu.

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Ethical Approval

This study was performed in accordance with the Declaration of Helsinki and approved by the Ethics Committee of Jiangsu Province Hospital of Chinese Medicine and Nanjing Medical University, Nanjing, China (No.2015NL-KS09). Written informed consent was obtained from the patients.

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Zhao, S., Qiu, X., Tan, Y. et al. Genomic Analysis and Molecular Characteristics in Carbapenem-Resistant Klebsiella pneumoniae Strains. Curr Microbiol 79, 391 (2022). https://doi.org/10.1007/s00284-022-03093-z

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