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Natronococcus pandeyae sp. nov., a Novel Haloarchaeon from Sambhar Salt Lake

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Abstract

A halophilic archaeon, designated strain LS1_42T, was isolated from Sambhar Salt Lake, Rajasthan, India. Cells were non-motile, coccoid, Gram-stain-variable and present in irregular clusters with light pink pigmented colonies. The strain was strictly aerobic and able to grow without Mg2+. Growth of the strain LS1_42T was observed at 25–45 °C, pH 7.0–11.0 and NaCl concentrations of 10–35% (w/v). The nearest phylogenetic neighbor of strain LS1_42T was Natronococcus amylolyticus Ah-36T based on 16S rRNA and rpoB′ genes with similarity of 95.4% and 91.9%, respectively. Phylogenetic analysis based on 16S rRNA gene, rpoB′ gene and whole-genome sequences indicate that the strain LS1_42T belongs to the genus Natronococcus and is closely related to N. amylolyticus. The genome size was 5.38 Mb with 98.9% completeness. The DNA G + C content of the strain LS1_42T was 63.0 mol%. The average nucleotide identity, average amino acid identity and DNA–DNA hybridization values between LS1_42T and N. amylolyticus Ah-36T were 81.3%, 77.7% and 24.8%, respectively. The major polar lipids detected were phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester. On the basis of phenotypic, chemotaxonomic and genome-based analysis, strain LS1_42T represents a novel species within the genus Natronococcus, for which the name Natronococcus pandeyae sp. nov. is proposed. The type strain is LS1_42T (MCC 3654T = JCM 33003T = KCTC 4280T = CGMCC 1.16738T).

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Acknowledgements

We are grateful to Prof. Aharon Oren and Prof. Bernhard Schink for the Latin etymology and species epithet. We are grateful to Kunal Jani for his help in sample collection.

Funding

This work was supported by the Department of Biotechnology (DBT), Government of India (Grant No. BT/Coord.II/01/03/2016), under the project ‘Establishment of National Centre for Microbial Resource’.

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Authors and Affiliations

  1. National Centre for Cell Science, Pune, Maharashtra, 411007, India

    Swapnil Kajale, Tushar Lodha, Yogesh Shouche & Avinash Sharma

  2. Abasaheb Garware College, Pune, Maharashtra, 411004, India

    Swapnil Kajale & Neelima Deshpande

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Contributions

SK isolated the strain LS1_41T, performed phenotypic, biochemical, genomic and phylogenetic analysis and wrote the manuscript; TL performed the polar lipid analysis; ND, YS and AS supervised the study; AS designed study and revised the manuscript. All authors read and approved the final version of the manuscript.

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Correspondence to Avinash Sharma.

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Kajale, S., Deshpande, N., Lodha, T. et al. Natronococcus pandeyae sp. nov., a Novel Haloarchaeon from Sambhar Salt Lake. Curr Microbiol 79, 51 (2022). https://doi.org/10.1007/s00284-021-02740-1

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