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Complete Genome of Marine Microalgae Associated Algicidal Bacterium Sulfitobacter pseudonitzschiae H46 with Quorum Sensing System

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Abstract

Sulfitobacter pseudonitzschiae H46, a marine bacterium associated with microalgae, exhibits algicidal activity against harmful algal bloom microalgae Chattonella marina and Alexandrium tamarense. The complete genome of S. pseudonitzschiae H46 was sequenced to investigate its algicidal mechanism further. One chromosome, eight circular plasmids and add one not circularized plasmid (Plas II) were identified, which are 4.93 Mb in length with 4871 open reading frames. The genome contains 43 transfer RNA (tRNA) genes; six ribosome RNA (rRNA) genes that can be classified into two 5S rRNA, two 16S rRNA, two 23S rRNA, and two small RNA (sRNA) genes. Genomic annotation revealed that S. pseudonitzschiae H46 has two pairs of LuxI/R-type Quorum-sensing (QS) systems. The QS systems can regulate the functions involved in host colonization and biofilm formation. Moreover, the existence of 49 Type-IV and Dddl genes in the chromosome indicated that Dimethylsulfoniopropionate (DMSP) catabolism of S. pseudonitzschiae H46 is the main interaction with marine microalgae. S. pseudonitzschiae H46 utilizes DMSP produced by microalgae as the carbon source, and this may induce algicidal activity. The complete genome sequence of S. pseudonitzschiae H46 can provide useful information on the underlying molecular mechanism between QS and algicidal activity.

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Acknowledgements

This work was supported by Basic Scientific Fund for National Public Research Institutes of China (2021Q10), the National Natural Science Foundation of China (41806201, 41776176), the National Natural Science Foundation of China-Shandong Joint Funded Project (U1606404) and Open Fund of Key Laboratory of Biotechnology and Bioresources Utilization (Dalian Minzu University), Ministry of Education (KF202006), China.

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Authors and Affiliations

  1. Key Laboratory of Marine Eco-Environmental Science and Technology, Marine Bioresource and Environment Research Center, First Institute of Oceanography, Ministry of Natural Resources of China, 6# Xian Xia Ling Road, Qingdao, 266061, People’s Republic of China

    Tianyi Hu, Shuai Wang, Yanchao Zhang, Yunjin Zhu & Li Zheng

  2. Laboratory for Marine Ecology and Environmental Science, Qingdao Pilot National Laboratory for Marine Science and Technology, Qingdao, 266071, China

    Li Zheng

  3. Oceanic Consultation Center, Ministry of Natural Resources of China, Beijing, 100161, China

    Yishi Shan

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  1. Tianyi Hu
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  2. Shuai Wang
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  3. Yishi Shan
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  4. Yanchao Zhang
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  5. Yunjin Zhu
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  6. Li Zheng
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Correspondence to Shuai Wang or Li Zheng.

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Hu, T., Wang, S., Shan, Y. et al. Complete Genome of Marine Microalgae Associated Algicidal Bacterium Sulfitobacter pseudonitzschiae H46 with Quorum Sensing System. Curr Microbiol 78, 3741–3750 (2021). https://doi.org/10.1007/s00284-021-02632-4

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  • DOI: https://doi.org/10.1007/s00284-021-02632-4

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