Abstract
An aerobic, gram-stain-negative, pink-colored, non-motile and rod-shaped algicidal bacterium, designated as JA-25T was isolated from freshwater in Geumgang River, Republic of Korea. Strain JA-25T grew at 15–30 °C and pH 6–9, and did not require NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain JA-25T belongs to the family ‘Spirosomaceae’ and is most closely related to Fibrella aestuarina BUZ 2T (93.6%). Strain JA-25T showed < 90% sequence similarity to other members of the family ‘Spirosomaceae’. The average nucleotide identity(ANI), in silico DNA-DNA hybridization and average amino acid identity(AAI) values based on the genomic sequences of JA-25T and F. aestuarina BUZ 2T were 74.4, 20.5, and 73.6%, respectively. Strain JA-25T showed an algicidal effect on the marine flagellate alga Heterocapsa triquetra, but no effect on fresh water cyanobacterium (Nostoc). In genome analysis, RIPP-like peptides were detected and predicted to resemble the indolmycin biosynthetic gene cluster, which possibly influence its algicidal effect. Furthermore, a bacteriorhodopsin gene with photoheterotrophic characteristics was detected. The genomic DNA G + C content was 52.5 mol%. The major cellular fatty acids were summed feature 3 (C16:1 ω6c/C16:1 ω7c), C16:1 ω5c, C16:0 (> 10%). The major respiratory quinone was menaquinone 7 and major polar lipids were phosphatidylethanolamine, two unidentified aminolipids, two phospholipids, and five unidentified lipids. Considering the phylogenetic inference, phenotypic, and chemotaxonomic data, strain JA-25T should be classified as a novel species in the novel genus Fibrivirga, with the proposed name Fibrivirga algicola sp. nov. The type strain is JA-25T (= KCCM 43334T = NBRC 114259T).
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Acknowledgements
This study was supported by a grant from the Nakdonggang National Institute of Biological Resources(NNIBR), funded by the Ministry of Environment(MOE) of the Republic of Korea (NNIBR202101105) and also funded by the Korea Environmental Industry & Technology Institute (KEITI) through the Aquatic Ecosystem Conservation Research Program, funded by Korea Ministry of Environment (MOE) (2020003030006).
Funding
This study was supported by the Nakdonggang National Institute of Biological Resources (NNIBR), funded by the Ministry of Environment (MOE) of the Republic of Korea (NNIBR202102107) and Korea Environmental Industry & Technology Institute (KEITI) through the Aquatic Ecosystem Conservation Research Program, funded by Korea MOE (2020003030006).
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Investigation. SP, JYC, SWJ, SWN, DRK, JR, and JHE; writing—original draft, SP, JYC, and DJ; writing—review and editing, SP, and KTK; All authors have read and agreed to the published version of the manuscript.
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Park, S., Cho, J.Y., Jung, DH. et al. Fibrivirga algicola gen. nov., sp. nov., an algicidal bacterium isolated from a freshwater river. Antonie van Leeuwenhoek 115, 899–909 (2022). https://doi.org/10.1007/s10482-022-01743-7
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DOI: https://doi.org/10.1007/s10482-022-01743-7