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Antifungal Properties, Abiotic Stress Resistance, and Biocontrol Ability of Bacillus mojavensis PS17

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Abstract

Plant-protecting Bacillus sp. strains used as biocontrol agents frequently produce metabolites inhibiting phytopathogenic fungi. Recently, the search for a novel biocontrol agent with a wide spectrum of disease control drew attention to Bacillus subtilis and their related species, including Bacillus mojavensis. In this study, we determined the antifungal properties of the endophytic B. mojavensis PS17 isolated from wheat seeds. Metabolites produced by B. mojavensis PS-17 inhibit the growth of Fusarium graminearum, Fusarium oxysporum, Fusarium chlamydosporum, Ascochyta pisi, Alternaria alternate, Sclerotinia sclerotiorum, Verticillium dahliaee, and Epicoccum nigrum strains. B. mojavensis strain PS17 produces several hydrolytic enzymes, such as chitinase, β-glucanase, cellulase, lipase, and protease. Additionally, strain B. mojavensis PS17 demonstrates drought tolerance under osmotic pressure of −2.2 MPa and a moderate halotolerance in 5% (w/v) of NaCl. B. mojavensis PS17 on tomato seedlings was able to reduce lesions of Forl ZUM2407 by 48.11% ± 1.07, showing the potentials of B. mojavensis PS17 to be adapted as a biocontrol agent for agricultural use.

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Acknowledgements

The study was conducted with the financial support provided by the Ministry of Education and Science of the Russian Federation under the agreement numbers AAAA-A18-118031390148-1 and 121021600147-1. The unique identifier of the project is FMEG-2021-0003.

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Authors and Affiliations

  1. Center for Agroecological Research, Kazan State Agrarian University, Kazan, Russian Federation

    Roderic Gilles C. Diabankana, Radik I. Safin, Rustam M. Nizamov & Lilia Z. Karimova

  2. Tatar Scientific Research Institute of Agriculture, FRC Kazan Scientific Center, Russian Academy of Sciences, Kazan, Russian Federation

    Daniel M. Afordoanyi

  3. Tatar Scientific Research Institute of Agrochemistry and Soil Science, FRC Kazan Scientific Center, Russian Academy of Sciences, Kazan, Russian Federation

    Daniel M. Afordoanyi & Shamil Z. Validov

  4. Laboratory of Chemical-Biological Research, Arbuzov IOPC FRC Kazan Scientific Center, Russian Academy of Sciences, Kazan, Russian Federation

    Roderic Gilles C. Diabankana

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  1. Roderic Gilles C. Diabankana
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  4. Rustam M. Nizamov
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Contributions

DRGC and ADM carried out the experimental work, statistical analysis, and drafting of the manuscript. B. mojavensis PS17 strain was isolated by KLZ, SRI, and NRM participated in the design of the experiment. VSZ contributed to the design and supervision of the study and also the editing of the manuscript. All the authors read and approved the manuscript.

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Correspondence to Roderic Gilles C. Diabankana.

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Conflict of interest

The authors declare that they have no conflict of interest. Five of the authors, including the corresponding author, hold a patent relating to the organism, Bacillus mojavensis PS17 with the publication number RU0002737208 (https://patents.google.com/patent/RU2737208C1/en?oq=Ru2737208) and also (https://patentscope.wipo.int/search/en/detail.jsf?docId=RU312423747&docAn=20191417).

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Diabankana, R.G.C., Afordoanyi, D.M., Safin, R.I. et al. Antifungal Properties, Abiotic Stress Resistance, and Biocontrol Ability of Bacillus mojavensis PS17. Curr Microbiol 78, 3124–3132 (2021). https://doi.org/10.1007/s00284-021-02578-7

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