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Halomonas azerica sp. nov., Isolated from Urmia Lake in Iran

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Abstract

A novel moderately halophilic, Gram-staining negative and facultative aerobic bacterium, designated as TBZ9T, was isolated from water of Urmia Lake in Azerbaijan region of Iran. The cells were found to be rod-shaped and motile, growing in the form of creamy, convex and shiny colonies. The strain could grow in the presence of NaCl at concentrations 1–17% (w/v) (optimum, 3%), temperatures 10–40 °C (optimum, 30 °C) and pH 6.0–11.0 (optimum, pH 7.0) on marine agar. Strain TBZ9T 16S rRNA gene sequence was related to the genus Halomonas showing highest similarities to Halomonas arcis AJ282T (98.4%), Halomonas songnenensis NEAU-ST10-39T (98.0%) and Halomonas lutescens Q1UT (97.8%). In the phylogenetic trees, strain TBZ9T formed a distinct branch closely related to a subclade inside the Halomonas genus. Digital DNA-DNA hybridization (dDDH) and average nucleotide identity (ANI) values between strain TBZ9T and H. arcis AJ282T (20.0%, 74.0%) and H. songnenensis NEAU-ST10-39T (19.8%, 75.2%) indicated that TBZ9T represents a distinct species. Evaluation of fatty acid contents determined C10:0, C16:0, C12:0 3-OH, summed feature 3 (C16:1 ω7c and/or C16:1 ω6c) and summed feature 8 (C18:1 ω7c and/or C18:1 ω6c) as major cellular fatty acids. The major quinone of strain TBZ9T was Q-9. Polar lipid patterns consisted of phosphatidylglycerol (PG), phosphatidylethanolamine (PE), two unidentified phospholipids (PL) and four unidentified polar lipids (L). The average DNA G + C content of strain TBZ9T is 55.4 mol%. Results from phenotypic, chemotaxonomic and molecular analysis suggest that the strain TBZ9T represents a novel species within the genus Halomonas for which the name Halomonas azerica sp. nov. is proposed. The type strain is TBZ9T (= KACC 21783T = LMG 25775T).

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References

  1. Franzmann PD, Wehmeyer U, Stackebrandt E (1988) Halomonadaceae fam. nov., a new family of the class proteobacteria to accommodate the genera Halomonas and Deleya. Syst Appl Microbiol 11(1):16–19

    Article  Google Scholar 

  2. Vreeland RH, Litchfield C, Martin E, Elliot E (1980) Halomonas elongata, a new genus and species of extremely salt-tolerant bacteria. Int J Syst Evol Microbiol 30(2):485–495

    CAS  Google Scholar 

  3. Lee J-C, Jeon CO, Lim J-M, Lee S-M, Lee J-M, Song S-M, Park D-J, Li W-J, Kim C-J (2005) Halomonas taeanensis sp. nov., a novel moderately halophilic bacterium isolated from a solar saltern in Korea. Int J Syst Evol Microbiol 55(5):2027–2032

    Article  CAS  Google Scholar 

  4. Khan SA, Zununi Vahed S, Forouhandeh H, Tarhriz V, Chaparzadeh N, Hejazi MA, Jeon CO, Hejazi MS (2020) Halomonas urmiana sp. nov., a moderately halophilic bacterium isolated from Urmia lake in Iran. Int J Syst Evol Microbiol 70(4):2254–2260

    Article  CAS  Google Scholar 

  5. Lee J-C, Kim Y-S, Yun B-S, Whang K-S (2015) Halomonas salicampi sp. nov., a halotolerant and alkalitolerant bacterium isolated from a saltern soil. Int J Syst Evol Microbiol 65(12):4792–4799

    Article  CAS  Google Scholar 

  6. Xu L, Xu X-W, Meng F-X, Huo Y-Y, Oren A, Yang J-Y, Wang C-S (2013) Halomonas zincidurans sp. nov., a heavy-metal-tolerant bacterium isolated from the deep-sea environment. Int J Syst Evol Microbiol 63(11):4230–4236

    Article  CAS  Google Scholar 

  7. Vahed SZ, Forouhandeh H, Tarhriz V, Chaparzadeh N, Hejazi MA, Jeon CO, Hejazi MS, Lee Y (2018) Halomonas tabrizica sp. nov., a novel moderately halophilic bacterium isolated from Urmia lake in Iran. Antonie Van Leeuwenhoek 111(7):1139–1148

    Article  CAS  Google Scholar 

  8. Lee J-C, Kim S-J, Whang K-S (2016) Halomonas sediminicola sp. nov., a moderately halophilic bacterium isolated from a solar saltern sediment. Int J Syst Evol Microbiol 66(10):3865–3872

    Article  CAS  Google Scholar 

  9. Oguntoyinbo FA, Cnockaert M, Cho G-S, Kabisch J, Neve H, Bockelmann W, Wenning M, Franz CM, Vandamme P (2018) Halomonas nigrificans sp. nov., isolated from cheese. Int J Syst Evol Microbiol 68(1):371–376

    Article  CAS  Google Scholar 

  10. Wang Y-F, Wang X-L, Li H, Lin K-F, Wang P, Yang J, Liu Y-D, Sun Z-Y, Fan L-H, Wu Z-M (2014) Treatment of high salinity phenol-laden wastewater using a sequencing batch reactor containing halophilic bacterial community. Int Biodeterior Biodegrad 93:138–144

    Article  CAS  Google Scholar 

  11. Monzón GC, Nisenbaum M, Seitz MKH, Murialdo SE (2018) New findings on aromatic compounds’ degradation and their metabolic pathways, the biosurfactant production and motility of the halophilic bacterium Halomonas sp. KHS3. Curr Microbiol 75(8):1108–1118

    Article  Google Scholar 

  12. Xu P, Li W-J, Tang S-K, Zhang Y-Q, Chen G-Z, Chen H-H, Xu L-H, Jiang C-L (2005) Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’isolated from China. Int J Syst Evol Microbiol 55(3):1149–1153

    Article  CAS  Google Scholar 

  13. Hamidi-Razi H, Mazaheri M, Carvajalino-Fernández M, Vali-Samani J (2019) Investigating the restoration of lake Urmia using a numerical modelling approach. J Great Lakes Res 45(1):87–97

    Article  Google Scholar 

  14. Lanyi B (1988) Classical and rapid identification methods for medically important bacteria. In: Colwell RR, Grigorova R (eds) Methods in microbiology, vol 19. Elsevier, New York, pp 1–67

    Google Scholar 

  15. Stackebrandt E, Goodfellow M (1991) Nucleic acid techniques in bacterial systematics. Wiley, Hoboken

    Google Scholar 

  16. Tarhriz V, Mohammadzadeh F, Hejazi MS, Nematzadeh G, Rahimi E (2011) Isolation and characterization of some aquatic bacteria from Qurugol lake in Azerbaijan under aerobic conditions. Adv Environ Biol 5:3173–3179

    CAS  Google Scholar 

  17. Kumar S, Stecher G, Tamura K (2016) MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33(7):1870–1874

    Article  CAS  Google Scholar 

  18. Wang Q, Garrity GM, Tiedje JM, Cole JR (2007) Naive bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. Appl Environ Microbiol 73(16):5261–5267

    Article  CAS  Google Scholar 

  19. Yoon S-H, Ha S-m, Lim J, Kwon S, Chun J (2017) A large-scale evaluation of algorithms to calculate average nucleotide identity. Antonie Van Leeuwenhoek 110(10):1281–1286

    Article  CAS  Google Scholar 

  20. Meier-Kolthoff JP, Auch AF, Klenk H-P, Göker M (2013) Genome sequence-based species delimitation with confidence intervals and improved distance functions. BMC Bioinform 14(1):60

    Article  Google Scholar 

  21. Aziz RK, Bartels D, Best AA, DeJongh M, Disz T, Edwards RA, Formsma K, Gerdes S, Glass EM, Kubal M (2008) The RAST server: rapid annotations using subsystems technology. BMC Genom 9(1):1–15

    Article  Google Scholar 

  22. Minnikin D, O’donnell A, Goodfellow M, Alderson G, Athalye M, Schaal A, Parlett J (1984) An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. J Microbiol Methods 2(5):233–241

    Article  CAS  Google Scholar 

  23. Chun J, Oren A, Ventosa A, Christensen H, Arahal DR, da Costa MS, Rooney AP, Yi H, Xu X-W, De Meyer S (2018) Proposed minimal standards for the use of genome data for the taxonomy of prokaryotes. Int J Syst Evol Microbiol 68(1):461–466

    Article  CAS  Google Scholar 

  24. Jiang J, Pan Y, Hu S, Zhang X, Hu B, Huang H, Hong S, Meng J, Li C, Wang K (2014) Halomonas songnenensis sp. nov., a moderately halophilic bacterium isolated from saline and alkaline soils. Int J Syst Evol Microbiol 64(5):1662–1669

    Article  CAS  Google Scholar 

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Funding

This work was supported by National Research Foundation (Grant No. 2017M3C1B5019250) of the Ministry of Science and ICT, Republic of Korea and Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran, (Pazhoohan ID: 66068 and Code of: IR.TBZMED.VCR.REC.1399.253).

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Author notes

  1. Ruan Wenting and Soheila Montazersaheb have contributed equally in this study and should be considered as co-first authors.

Authors and Affiliations

  1. Department of Life Science, Chung Ang University, 84, HeukSeok-Ro, Dongjak-Gu, Seoul, 06974, Republic of Korea

    Ruan Wenting, Shehzad Abid Khan, Hyung Min Kim & Che Ok Jeon

  2. Molecular Medicine Research Center, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran

    Soheila Montazersaheb, Vahideh Tarhriz & Mohammad Saeid Hejazi

  3. Branch for the Northwest and West Region, Agriculture Biotechnology Research Institute of Iran (ABRII), Tabriz, Iran

    Mohammad Amin Hejazi

  4. Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz, Iran

    Mohammad Saeid Hejazi

Authors
  1. Ruan Wenting
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  2. Soheila Montazersaheb
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  3. Shehzad Abid Khan
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  4. Hyung Min Kim
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  6. Mohammad Amin Hejazi
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  7. Mohammad Saeid Hejazi
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  8. Che Ok Jeon
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Contributions

COJ and MSH conceived the ideas and supervised all works. VH and MAH collected the samples, isolated the strain and performed initial cultivation, storage and deposition. RW and SM analyzed the phenotypic, biochemical and genomic properties. HMK sequenced and analysed the genome. SM, SAK and COJ wrote the manuscript and the manuscript has been reviewed and edited by all authors.

Corresponding authors

Correspondence to Mohammad Saeid Hejazi or Che Ok Jeon.

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Wenting, R., Montazersaheb, S., Khan, S.A. et al. Halomonas azerica sp. nov., Isolated from Urmia Lake in Iran. Curr Microbiol 78, 3299–3306 (2021). https://doi.org/10.1007/s00284-021-02482-0

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  • DOI: https://doi.org/10.1007/s00284-021-02482-0

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