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Chelativorans alearense sp. nov., A Novel Bacterial Species Isolated From Soil in Alear, China

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Abstract

A novel Gram-strain-negative, rod-shaped, non-flagellated, non-gliding, beige-pigmented and aerobic bacterium, designated strain UJN715T, was isolated from rhizosphere soil of Alhagi sparsifolia obtained from Alear city, located in Xinjiang province, PR China. Growth optimally occurred at 37 °C, pH 6.5–7.5, and 0–3% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequence showed that strain UJN715T belonged to the genus Chelativorans, with the highest sequence similarity to Chelativorans multitrophicus DSM 9103 T (97.7%). Genome sequencing revealed a genome size of 5 702 301 bp and a G + C content of 64.1 mol%. The ANI, POCP and the dDDH between strain UJN715T and C. multitrophicus DSM 9103 T were 76.2%, 49.3%, and 20.5%, respectively. The prediction result of secondary metabolites based on genome showed that the strain UJN715T contained one cluster of ectoine production, one cluster of non-ribosomal peptide synthetase (NRPS), one cluster of type I polyketide synthases (TIPKS), one cluster of bacteriocin, one cluster of TfuA-related, one cluster of N-acetylglutaminylglutamine amide (NAGGN) production, one cluster of terpene production, two clusters of homoserine lactone (Hserlactone) production. The major respiratory quinone was Q-10. The major fatty acids were iso-C17:0, C18:0 and C19:0 cyclo ω8c and its polar lipids consisted of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, phospholipids, unknown lipids, diphosphatidylglycerol, aminoglycolipid, unidentified aminophospholipids. On the basis of these data, strain UJN715T is considered to represent a novel species of the genus Chelativorans, for which the name Chelativorans alearense sp. nov. is proposed. The type strain is UJN715T (= KCTC 72856T = CCTCC AB2019378T).

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Abbreviations

ANI:

Average nucleotide identity

dDDH:

Digital DNA–DNA hybridization

HPLC:

High-performance liquid chromatography

POCP:

Percentage of conserved proteins

MIDI:

Microbial Identification System

KCTC:

Korean Collection for Type Cultures

CCTCC:

China Center for Type Culture Collection

TLC:

Thin layer chromatography

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Acknowledgements

This work was supported by the National Natural Science Foundation of China (32070111, 31870105, 31741007), and the Training Foundation of Shandong Natural Science Foundation (ZR2019PEE030).

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Authors and Affiliations

  1. School of Biological Science and Technology, University of Jinan, Jinan, 250022, People’s Republic of China

    Dong Meng, Yu-Ling Liu, Peng-Fei Gu, Xiang-Yu Fan, Zhao-Song Huang, Yan Ji & Qiang Li

  2. Key Laboratory for Northern Urban, Agriculture of Ministry of Agriculture and Rural Affairs, Beijing University of Agriculture, Beijing, 102206, People’s Republic of China

    Wei-Min Li

  3. Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, People’s Republic of China

    Wei-Min Li

  4. Marine College, Shandong University, Weihai, 264209, People’s Republic of China

    Zong-Jun Du

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  1. Dong Meng
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Contributions

MD, LWM, and LQ designed research and project outline. LYL, GFP, and HZS performed isolation, deposition, and identification. JY, FXY, DZJ, LWM, and LQ drafted the manuscript. All authors read and approved the final manuscript.

Corresponding authors

Correspondence to Wei-Min Li or Qiang Li.

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The authors declare that they have no conflict of interest.

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Informed consent was obtained from all individual participants included in the study.

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This article does not contain any studies with animals performed by any of the authors.

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New Taxa—Proteobacteria

The GenBank accession number for the 16S rRNA gene sequence of Chelativorans alearense UJN715T is MN744315. The whole-genome shotgun project of strain UJN715T has been deposited at DDBJ/ENA/GenBank under the accession number WQNI00000000. The version described in this paper is version WQNI00000000.

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Meng, D., Liu, YL., Gu, PF. et al. Chelativorans alearense sp. nov., A Novel Bacterial Species Isolated From Soil in Alear, China. Curr Microbiol 78, 1656–1661 (2021). https://doi.org/10.1007/s00284-021-02428-6

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  • DOI: https://doi.org/10.1007/s00284-021-02428-6

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